Abstract
Loss of the long arm of chromosome 18 (18q) is one of the most common genetic changes in colorectal cancer. This chapter describes the method to determine chromosome 18q status using microsatellite markers. Specifically, tumor and normal tissue are separated by microdissection of routine formalinfixed paraffin embedded tissues obtained from surgical resection. Total genomic DNA are isolated and subjected to polymerase chain reaction (PCR) using polymorphic microsatellite markers located on the long arm of chromosome 18 (1).
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Reference
Jen, J., Kim, H., Piantadosi, S., Liu, Z.-F., Levitt, R. C., Sistonen, P., Kinzler, K. W., Vogelstein, B., and Hamilton, S. R. (1994) Allelic loss of chromosome 18q and prognosis in colorectal cancer. New Engl. J. Med. 331, 213–221.
Beckman, J. S. and Weber, J. L. (1992) Survey of human and rat microsatellites. Genomics 12, 627–631.
NIH/CEPH collaborative mapping group. (1992) A comprehensive genetic link-age map of the human genome. Nature 258, 67–86.
Saiki, R. K., Gelfand, D. H., Stoffel, S., et al. (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239, 487–491.
Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989) Molecular Cloning-A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, pp. 13. 45–13.57.
Litt, M., Hauge, X., and Sharma, V. (1993) Shadow bands seen when typing polymorphic dinucleotide repeats: some causes and cures. Biotechniques 15, 280–284.
Louis, D. N., Deimling, A., and Seizinger, B. R. (1992) A (CA)n dinucleotide repeat assay for evaluating loss of allelic heterozygosity in small and archival human brain tumor specimens. Am. J. Pathol. 141, 777–782.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2001 Humana Press Inc.
About this protocol
Cite this protocol
Jen, J. (2001). Microsatellite Analysis to Assess Chromosome 18q Status in Colorectal Cancer. In: M., S. (eds) Colorectal Cancer. Methods in Molecular Medicine, vol 50. Humana Press. https://doi.org/10.1385/1-59259-084-5:51
Download citation
DOI: https://doi.org/10.1385/1-59259-084-5:51
Publisher Name: Humana Press
Print ISBN: 978-0-89603-767-0
Online ISBN: 978-1-59259-084-1
eBook Packages: Springer Protocols