Advertisement

Genetic Analysis Using Microarrays

  • Wa’el El-Rifai
  • Sakari Knuutila
Part of the Methods in Molecular Medicine book series (MIMM, volume 50)

Abstract

A vast amount of genome sequencing data has become available over the past few years and methods to facilitate high-throughput analysis of large sets of genes and samples have been developed to localize novel genes related to human cancer. As advanced robotic applications have made it possible to manufacture high-precision microarrays on glass or membranes, pioneering scientists have introduced several variants of the “array” technology: oligonucleotide arrays (1), DNA microarrays (CGH arrays) (2), tissue microarrays (3), and cDNA microarrays (4,5). The array technology is based on fluorescently (glass-based arrays, chips) or radioactively (filter-based array) labeled nucleic acids that are hybridized to the microarray and imaged with a laser scanner or a phosphor imager, respectively. The images are then processed using microarray analysis software. These techniques have recently been reviewed in detail in Nature Genetics (vol. 21, Suppl. 1, 1999).

Keywords

Comparative Genomic Hybridization Image Plate Comparative Genomic Hybridization Array DNase Treatment Isoamyl Alcohol 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Pease, A. C., Solas, D., Sullivan, E. J., Cronin, M. T., Holmes, C. P., and Fodor, S. P. (1994) Light-generated oligonucleotide arrays for rapid DNA sequence analysis. Proc. Natl. Acad. Sci. USA 91, 5022–5026.CrossRefPubMedGoogle Scholar
  2. 2.
    Pinkel, D., Segraves, R., Sudar, D., Clark, S., Poole, I., Kowbel, D., et al. (1998) High resolution analysis of DNA copy number variation using comparative genomic hybridization to microarrays. Nat. Genet. 20, 207–211.CrossRefPubMedGoogle Scholar
  3. 3.
    Kononen, J., Bubendorf, L., Kallioniemi, A., Barlund, M., Schraml, P., Leighton, S., et al. (1998) Tissue microarrays for high-throughput molecular profiling of tumor specimens. Nat. Med. 4, 844–847.CrossRefPubMedGoogle Scholar
  4. 4.
    Schena, M., Shalon, D., Davis, R. W., and Brown, P. O. (1995) Quantitative monitoring of gene expression patterns with a complementary DNA microarray [see comments]. Science 270, 467–470.CrossRefPubMedGoogle Scholar
  5. 5.
    Shalon, D., Smith, S. J., and Brown, P. O. (1996) A DNA microarray system for analyzing complex DNA samples using two-color fluorescent probe hybridization. Genome Res. 6, 639–645.CrossRefPubMedGoogle Scholar
  6. 6.
    Hacia, J. G., Brody, L. C., Chee, M. S., Fodor, S. P., and Collins, F. S. (1996) Detection of heterozygous mutations in BRCA1 using high density oligonucleotide arrays and two-colour fluorescence analysis. Nat. Genet. 14, 441–447.CrossRefPubMedGoogle Scholar
  7. 8.
    Pietu, G., Alibert, O., Guichard, V., Lamy, B., Bois, F., Leroy, E., et al. (1996) Novel gene transcripts preferentially expressed in human muscles revealed by quantitative hybridization of a high density cDNA array. Genome Res. 6, 492–503.CrossRefPubMedGoogle Scholar

Copyright information

© Humana Press Inc. 2001

Authors and Affiliations

  • Wa’el El-Rifai
    • 1
  • Sakari Knuutila
    • 1
  1. 1.Department of Medical Genetics, Haartman Institute and Helsinki University Central HospitalUniversity of HelsinkiFinland

Personalised recommendations