Abstract
Transcutaneous immunization (TCI), the introduction of antigens using a topical application to intact skin, is a new technology that has both practical and immunological merits. Practically speaking, a needle-free method of vaccine delivery will decrease the risk of needle-borne diseases, reduce the complications related to physical skin penetration, improve access to vaccination by eliminating the need for trained personnel and sterile equipment, and provide a simple means for multivalent or multiple boosting immunization. The immunological implications of TCI are profound as this technique appears to target highly accessible antigen presenting cells (APC) in the skin that can be exploited for a variety of immune outcomes. It has been our experience that TCI can be reliably and reproducibly conducted with a variety of antigens to induce potent and functional immune responses. Thus, this new method may significantly impact both the delivery of vaccines and open new possibilities for manipulation of the immune response.
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References
Lycke, N. (1997) The mechanism of cholera toxin adjuvanticity. Res. Immunol. 148, 504ā520.
Snider, D. P. (1995) The mucosal adjuvant activities of ADP-ribosylating bacterial enterotoxins. Crit. Rev. Immunol. 15, 317ā348.
Elson, C. O. and Ealding, W. (1984) Cholera toxin feeding did not induce oral tolerance in mice and abrogated oral tolerance to an unrelated protein antigen. J. Immunol. 133, 2892ā2897.
Dickinson, B. L. and Clemens, J. D. (1995) Dissociation of Escherichia coliheat-labile enterotoxin adjuvanticity from ADP-ribosyltransferase activity. Infect. Immun. 63, 1617ā1623.
Lobet, Y., Cluff, C. W., and Cieplak, W. (1991) Effect of site-directed mutagenic alterations on ADP-ribosyltransferase activity of the A subunit of Escherichia coli heat-labile enterotoxin. Infect. Immun. 59, 2870ā2879.
Holmgren, J., Svennerholm, A-M., Jertborn, M., Clemens, J., Sack, D. A., Salenstedt, R., and Wigzell, H. (1992) An oral B subunit: whole cell vaccine against cholera. Vaccine 10, 911ā914.
Tamura, S., Funato, H., Nagamine, T., Aizawa, C., and Kurata, T. (1989) Effectiveness of cholera toxin B subunit as an adjuvant for nasal influenza vaccination despite pre-existing immunity to CTB. Vaccine 7, 503ā505.
Glenn, G. M., Rao, M., Matyas, G. R., and Alving, C. R. (1998) Skin immunization made possible by cholera toxin. Nature 391, 851.
Kripke, M. L., Munn, C. G., Jeevan, A., Tang, J. M., and Bucana, C. (1990) Evidence that cutaneous antigen-presenting cells migrate to regional lymph nodes during contact sensitization. J. Immunol. 145, 2833ā2838.
News and views (1998) Nature Medicine 4, 672.
Udey, M. C. (1997) Cadherins and Langerhans cell immunobiology. Clin. Exp. Immunol. 107(Suppl. 1), 6ā8.
Roberts, M. S. and Walker, M. (1993) Water the most natural penetration enhancer, in Pharmaceutical Skin Penetration Enhancement (Walters, K. A. and Hadgraft, J., eds.), Marcel Dekker, New York, pp. 1ā30.
Holbrook, K. A. and Odland, D. F. (1974) Regional differences in the thickness (cell layers) of the human stratum corneum: an ultrastructural analysis. J. Invest. Dermatol. 62, 415ā422.
Berman, B., Chen, V. L., France, D. S., Dotz, W. I., and Petroni, G. (1983) Anatomical mapping of epidermal Langerhans cells densities in adults. Br. J. Dermatol. 109, 553ā558.
Bergstresser, P. R., Fletcher, C. R., and Streilein, J. W. (1980) Surface densities of Langerhans cells in relation to rodent epidermal sites with special immunologi-cal properties. J. Invest. Dermatol. 74, 77ā80.
Schweizer, J. (1980) Langerhans cell-free regions in orthokeratinizing sole-of-foot epidermis of the adult mouse. Arch. Dermatol. Res. 268, 157ā166.
Fisherās Contact Dermatitis, 4th ed. (1995) (Rietschel, R. L. and Fowler, J. F., eds.), Williams & Wilkins, Baltimore, MD, p. 2.
Glenn, G. M., Scharton-Kersten, T., Vassell, R., Matyas, G. R., and Alving, C. R. (1999) Transcutaneous immunization using bacterial ADP-ribosylating exotoxins as antigens and adjuvants. Infect. Immun. 67, 1000ā1006.
Glenn, G. M., Scharton-Kersten, T., Vassell, R., Mallet, C. P., Hale, T. L., and Alving, C. R. (1998) Transcutaneous immunization with cholera toxin protects mice against lethal mucosal toxin challenge. J. Immunol. 161, 3212ā3214.
Fairweather, N. F., Lyness, V. A., and Maskell, D. J. (1987) Immunization of mice against tetanus with fragments of tetanus toxin synthesized in Escherichia coli. Infect. Immun. 55, 2541ā2545.
McComb, J. A. (1964) The prophylactic dose of homologous tetanus antitoxin. N. Engl. J. Med. 270, 175ā178.
Schneerson, R., Robbins, J. B., Taranger, J., Lagergard, T., and Trollfors, B. (1996) A toxoid vaccine for pertussis as well as diphtheria? Lessons to be relearned. Lancet 348, 1289ā1292.
Goldblatt, D., Vaz, A. R., and Miller, E. (1998) Antibody avidity as a surrogate maker of successful priming by Haemophilus influenzae type b conjugate vaccines following infant immunization. J. Infect. Dis. 177, 1112ā1115.
Paul, A. and Cevc, G. (1995) Noninvasive administration of protein antigens: Transdermal immunization with bovine serum albumin in transferasomes. Vaccine Res. 3, 145ā164.
Tang, D. C., Shi, Z., and Curiel, D. T. (1997) Vaccination onto bare skin. Nature 388, 729ā730.
Wang, L., Lin, J., Hsieh, K., and Lin, R. (1996) Epicutaneous exposure of protein antigen indices a predominant Th2-like response with high IgE production in mice. J. Immunol. 156, 4077ā4082.
Scharton-Kersten, T., Glenn, G. M., Vassell, R., Yu, J., Walwender, D., and Alving, C. R. (1999) Principles of transcutaneous immunization using cholera toxin as an adjuvant. Vaccine 17(Suppl), 537ā543.
Hammond, S. A., Tsonis, C., Sellins, K., Rushlow, K., Scharton-Kersten, T., Colditz, I., and Glenn, G. M. (2000) Transcutaneous immunization of domestic animals: opportunities and challenges. Adv. Drug Delivery Rev., in press.
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Glenn, G.M., Scharton-Kersten, T., Vassell, R. (2000). Transcutaneous Immunization. In: OāHagan, D.T. (eds) Vaccine Adjuvants. Methods in Molecular Medicineā¢, vol 42. Springer, Totowa, NJ. https://doi.org/10.1385/1-59259-083-7:315
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DOI: https://doi.org/10.1385/1-59259-083-7:315
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