Abstract
Celiac disease is characterized by mucosal changes in the small intestine, ranging from increased numbers of intraepithelial lymphocytes to complete villus effacement with various signs of malabsorption (1,2). Celiac disease is diagnosed by the demonstration of an altered intestinal mucosa in a jejunal biopsy specimen (3). Celiac disease patients invariably have antibodies directed against gliadin and endomysium, a structural component of the extracellular matrix: both antibodies disappear under a gluten-free diet. Therefore, these antibodies are useful tools for diagnosis, and in the dietary control of coeliac disease (3–7). Serum IgA antibodies against endomysial antibodies (EMAs) are especially considered to be sensitive and highly specific markers for celiac disease (8)(9). Previously, EMAs were detected by indirect immunofluorescence on tissue slides of monkey esophagus or human umbilical cord (10–12).
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References
Trier, J. S. (1991) Celiac sprue. N. Engl. J. Med. 325, 1709–1719.
Marsh, M. N. (1992) Gluten, major histocompatibility complex, and the small intestine: a molecular and immunobiologic approach to the spectrum of gluten sensitivity (‘celiac sprue’). Gastroenterology 102, 330–354.
Walker-Smith, J. A., Guandalini, S., Schmitz, J., Shmerling, D. H., and Visakorpi, J. K. (1990) Revised criteria for diagnosis of coeliac disease. Arch. Dis. Child. 65, 909–911.
Bürgin-Wolff, A., Gaze, H., Hadziselimovic, F., Huber, H., Lentze, M. J., Nusslé, D., and Reymond-Berthet, C. (1991) Antigliadin and antiendomysium antibody determination for coeliac disease. Arch. Dis. Child. 66, 941–947.
McMillan, S. A., Haughton, D. J., Biggart, J. D., Edgar, J. D., Porter, K. G, and McNeill, T. A. (1991) Predictive value for coeliac disease of antibodies to gliadin, endomysium, and jejunum in patients attending for jejunal biopsy. Br. Med. J. 303, 1163–1165.
Lindquist, B. L., Rogozinski, T., Moi, H., Danielsson, D., and Olcen, P. (1994) Endomysium and gliadin IgA antibodies in children with coeliac disease. Scand. J. Gastroenterol. 29, 452–456.
Corrao, G., Corazza, G. R., Andreani, M. L., Torchio, P., Valentini, R. A., Galatola, G., Quaglino, D., Gasbarrini, G., and di Orio, F. (1994) Serological screening of coeliac disease: choosing the optimal procedure according to various prevalence values. Gut 35, 771–775.
Volta, U., Molinaro, N., Fusconi, M., Cassani, F., and Bianchi, F. B. (1991) IgA antiendomysial antibody test: a step forward in celiac disease screening. Dig. Dis. Sci. 36, 752–756.
Lerner, A., Kumar, V., and Iancu, T. C. (1994) Immunological diagnosis of childhood coeliac disease: comparison between antigliadin, antireticulin and antiendomysial antibodies. Clin. Exp. Immunol. 95, 78–82.
Chorzelski, T. P., Beutner, E. H., Sulej, J., Tchorzewska, H., Jablonska, S., Kumar, V., and Kapuscinska, A. (1984) IgA anti-endomysium antibody: a new immunological marker of dermatitis herpetiformis and coeliac disease. Br. J. Dermatol. 111, 395–402.
Ladinser, B., Rossipal, E., and Pittschieler, K. (1994) Endomysium antibodies in coeliac disease: an improved method. Gut 35, 776–778.
Sategna-Guidetti, C., Bruna Grosso, S., Bruno, M., and Grosso, S. (1997) Is human umbilical cord the most suitable substrate for the detection of endomysium antibodies in the screening and follow-up of coeliac disease? Eur. J. Gastroenterol. Hepatol. 9, 657–660.
Dieterich, W., Ehnis, T., Bauer, M., Donner, P., Volta, U., Riecken, E. O., and Schuppan, D. (1997) Identification of tissue transglutaminase as the autoantigen of celiac disease. Nat. Med. 3, 797–801.
Gentile, V., Saydak, M., Chiocca, E. A., Akande, O., Birckbichler, P. J., Lee, K. N., Stein, J. P., and Davies, P. J. A. (1991) Isolation and characterization of cDNA clones to mouse macrophage and human endothelial cell tissue transglutaminases. J. Biol. Chem. 266, 478–483.
LeMosy, E. K., Erickson, H. P., Beyer, W. F., Radek, J. T., Jeong, J. M., Murthy, S. N. P., and Lorand, L. (1992) Visualization of purified fibronectintransglutaminase complexes. J. Biol. Chem. 267, 7880–7885.
Barsigian, C., Stern, A. M., and Martinez, J. (1991) Tissue (type II) transglutaminase covalently incorporates itself, fibrinogen, or fibronectin into high molecular weight complexes on the extracellular surface of isolated hepatocytes. J. Biol. Chem. 266, 22,501–22,509.
Achyuthan, K. E., Goodell, R. J., Kennedye, J. R., Lee, K. N., Henley, A., Stiefer, J. R., and Birckbichler, P. J. (1995) Immunochemical analyses of human plasma fibronectin-cytosolic transglutaminase interactions. J. Immunol. Methods 180, 69–79.
Dieterich, W., Laag, E., Schöpper, H., Volta, U., Ferguson, A., Gillet, A., Riecken, E. O., and Schuppan, D. (1998) Autoantibodies to tissue transglutaminase as predictors of celiac disease. Gastroenterology 115, 1–6.
Sulkanen, S., Halttunen, T., Laurrila, K., Kolho, K. L., Korponay-Szabo, I. R., Sanilahti, E., Lollin, P., and Mäki, M. (1998) Tissue transglutaminase autoantibody enzyme-linked immunosorbent assay in detecting celiac disease. Gastroenterology 115, 6–13.
Bullock, S. L. and Walls, K. W. (1977) Evaluation of some of the parameters of the enzyme-linked immunospecific assay. J. Infect. Dis. 136(Suppl.), S279–S285.
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Dieterich, W., Riecken, E.O., Schuppan, D. (2000). Immunoassay for Detection of IgA Antitissue Transglutaminase in Patients with Celiac Disease. In: Marsh, M.N. (eds) Celiac Disease. Methods in Molecular Medicine, vol 41. Humana Press. https://doi.org/10.1385/1-59259-082-9:241
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DOI: https://doi.org/10.1385/1-59259-082-9:241
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