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Celiac Disease pp 203–239Cite as

Immunohistochemistry in Research and Diagnosis of Celiac Disease

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Part of the book series: Methods in Molecular Medicine ((MIMM,volume 41))

Abstract

Immunohistochemistry comprises methods used to recognize tissue components as antigens in situ by means of directly or indirectly labeled antibodies, usually (but not always) derived from another species. When applied to cell preparations, the same methods are called immunocytochemistry (Fig. 1), although some authors also use this term for immunostaining of cellular components in tissue sections. Note that compared with immunostaining of vital cells in suspension or cultured monolayers, the sensitivity of immunohistochemical cell-surface staining is considerably reduced because of the decreased amounts of marker antigen represented by the cross-section of the plasma membrane. Also, for certain cellular markers examined in a tissue section, truly peripheral staining may be difficult to distinguish from a rim of cytoplasmic antigen expression.

Immunohistochemistry (A) is performed on tissue sections whereas immunocytochemistry (B) is performed on cells in suspensions, smears, or monolayers. Antibodies do not penetrate the surface membrane of living cells; therefore, unequivocal peripheral staining is obtained only for cells in suspension or vital monolayers. With tissue sections or dried (and fixed) cells, it is difficult to distinguish peripheral from cytoplasmic immunostaining unless the antigen is a distinct surface membrane marker.

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Author information

Authors and Affiliations

  1. Laboratory for Immunohistochemistry and Immunopathology (LIIPAT), Institute of Pathology,The National Hospital, Oslo, Norway

    Per Brandtzaeg & Frode L. Jahnsen

Authors
  1. Per Brandtzaeg
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  2. Frode L. Jahnsen
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Editor information

Editors and Affiliations

  1. Department of Medicine, University of Manchester, Salford, UK

    Michael N. Marsh MD, DSc, FRCP

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© 2000 Humana Press Inc., Totowa, NJ

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Brandtzaeg, P., Jahnsen, F.L. (2000). Immunohistochemistry in Research and Diagnosis of Celiac Disease. In: Marsh, M.N. (eds) Celiac Disease. Methods in Molecular Medicine, vol 41. Humana Press. https://doi.org/10.1385/1-59259-082-9:203

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  • DOI: https://doi.org/10.1385/1-59259-082-9:203

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-650-5

  • Online ISBN: 978-1-59259-082-7

  • eBook Packages: Springer Protocols

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