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Celiac Disease pp 175-183 | Cite as

In Situ Hybridization

  • Conrad G. Beckett
  • Paul J. Ciclitira
Part of the Methods in Molecular Medicine book series (MIMM, volume 41)

Abstract

In situhybridization permits specific identification of genes and gene expression without removing the target sequence from its topographical surroundings. The technique was described simultaneously in 1960 by two groups (1,2). It depends on the hybridization of a labeled nucleic acid probe to a complementary sequence of tissue mRNA or DNA.

Keywords

Celiac Disease Lamina Propria Acetic Anhydride Terminal Deoxynucleotidyl Transferase Dextran Sulfate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 2000

Authors and Affiliations

  • Conrad G. Beckett
    • 1
  • Paul J. Ciclitira
    • 2
  1. 1.Department of GastroenterologyBradford Royal InformaryBradfordUK
  2. 2.Department of GastroenterologyThe Rayne Institute,St.Thomas’HospitalLondonUK

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