Abstract
In routine histopathology, most tissues are fixed in formalin and embedded in paraffin for long-term preservation. DNA can be extracted from these tissues for subsequent molecular analysis by amplification methods. We describe herein a protocol for DNA preparation from paraffin-embedded tissues based on published procedures (1–3). In brief, tissue sections are placed into microfuge tubes, then deparaffinized with xylene. The xylene is removed with ethanol washes, and the tissue is treated with proteinase K to make DNA available for amplification.
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© 2001 Humana Press Inc.
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Fan, H., Gulley, M.L. (2001). DNA Extraction from Paraffin-Embedded Tissues. In: Killeen, A.A. (eds) Molecular Pathology Protocols. Methods in Molecular Medicine™, vol 49. Humana Press. https://doi.org/10.1385/1-59259-081-0:1
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DOI: https://doi.org/10.1385/1-59259-081-0:1
Publisher Name: Humana Press
Print ISBN: 978-0-89603-681-9
Online ISBN: 978-1-59259-081-0
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