Polymerase Chain Reaction Clonality Assays Based on X-Linked Genes

  • Langxing Pan
  • Huaizheng Peng
Part of the Methods in Molecular Medicine™ book series (MIMM, volume 49)


During embryogenesis in females, in each cell either the paternal or the maternal X chromosome is randomly inactivated through methylation (1 2). This event is stably inherited by daughter progeny of each cell. Therefore, in adult women, polyclonal cell populations will comprise a random mixture of paternally and maternally derived X-inactivated cells, but monoclonal cells will contain the same inactivated X chromosome as their progenitor. The paternal and maternal X chromosomes can be distinguished by identification of polymorphisms at certain alleles, and differences in methylation of DNA sequences within these alleles can be detected by digestion of the DNA with methylation-sensitive restriction enzymes. Based on these principles, researchers have developed several X-linked clonality assays, and most are performed using Southern blot hybridization (3, 4, 5).


Sodium Thiosulfate Solution Clonality Assay MAOA Gene Human Androgen Receptor Gene Polyclonal Cell Population 
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Copyright information

© Humana Press Inc. 2001

Authors and Affiliations

  • Langxing Pan
    • 1
  • Huaizheng Peng
    • 2
  1. 1.Department of PathologyWeill Medical College of Cornell UniversityNew York
  2. 2.Department of HistopathologyUniversisity College London Medical SchoolLondonUK

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