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Mechanistic Studies of Skin Electroporation Using Biophysical Methods

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Electrochemotherapy, Electrogenetherapy, and Transdermal Drug Delivery

Part of the book series: Methods in Molecular Medicine ((MIMM,volume 37))

Abstract

The mechanism by which high-voltage pulses transiently disrupt lipid bilayers in cell membranes has been the subject of controversy since electroporation was first observed almost three decades ago. Determining the mechanism by which such pulses permeabilize the complex, multilamellar bilayer structures in skin poses an even greater challenge. To address this issue, a range of methods have been employed to perform biophysical characterization for skin electroporation studies. In this chapter, we provide an overview of these methods and highlight representative findings which biophysical characterization has yielded.

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References

  1. Neumann, E., Sowers, A. E., and Jordan, C. A. (1989) Electroporation and Electrofusion in Cell Biology. Plenum, New York.

    Google Scholar 

  2. Tsong, T. Y. (1991) Electroporation of cell membranes. Biophys. J. 60, 297–306.

    Article  PubMed  CAS  Google Scholar 

  3. Chang, D. C., Chassy, B. M., Saunders, J. A., and Sowers, A. E. (1992) Guide to Electroporation and Electrofusion. Academic Press, New York.

    Google Scholar 

  4. Orlowski, S. and Mir, L. M. (1993) Cell electropermeabilization: A new tool for biochemical and pharmacological studies. Biochim. Biophys. Acta 1154, 51–63.

    PubMed  CAS  Google Scholar 

  5. Weaver, J. C. (1993) Electroporation: A general phenomenon for manipulating cells and tissues. J. Cell. Biochem. 51, 426–435.

    PubMed  CAS  Google Scholar 

  6. Bronaugh, R. L. and Maibach, H. I. (1999) Percutaneous Absorption, DrugsCosmetics—Mechanisms—Methodology. Marcel Dekker, New York.

    Google Scholar 

  7. Hadgraft, J. and Guy, R. H. (1989) Transdermal Drug Delivery: Developmental Issues and Research Initiatives. Marcel Dekker, New York.

    Google Scholar 

  8. Potts, R. O. and Guy, R. H. (1997) Mechanisms of Transdermal Drug Delivery. Marcel Dekker, New York.

    Google Scholar 

  9. Chizmadzhev, Y. A., Zarnytsin, V. G., Weaver, J. C., and Potts, R. O. (1995) Mechanism of electroinduced ionic species transport through a multilamellar lipid system. Biophys. J. 68, 749–765.

    Article  PubMed  CAS  Google Scholar 

  10. Edwards, D. A., Prausnitz, M. R., Langer, R., and Weaver, J. C. (1995) Analysis of enhanced transdermal transport by skin electroporation. J. Controlled Release 34, 211–221.

    Article  CAS  Google Scholar 

  11. Weaver, J. C., Vaughan, T. E., and Chizmadzhev, Y. (1999) Theory of electrical creation of aqueous pathways across skin transport barriers. Adv. Drug Deliv. Rev. 35, 21–39.

    Article  PubMed  CAS  Google Scholar 

  12. Prausnitz, M. R., Bose, V. G., Langer, R., and Weaver, J. C. (1993) Electroporation of mammalian skin: A mechanism to enhance transdermal drug delivery. Proc. Natl. Acad. Sci. USA 90, 10,504–10,508.

    Article  PubMed  CAS  Google Scholar 

  13. Pliquett, U., Langer, R., and Weaver, J. C. (1995) Changes in the passive electri cal properties of human stratum corneum due to electroporation. Biochim. Biophys. Acta 1239, 111–121.

    Article  PubMed  CAS  Google Scholar 

  14. Chizmadzhev, Y. A., Indenbom, A. V., Kuzmin, P. I., Galichenko, S. V., Weaver, J. C., and Potts, R. O. (1998) Electrical properties of skin at moderate voltages: Contribution of appendegeal macropores. Biophys. J. 74, 843–856.

    Article  PubMed  CAS  Google Scholar 

  15. Prausnitz, M. R. (1996) Do high-voltage pulses cause changes in skin structure? J. Controlled Release 40, 321–326.

    Article  CAS  Google Scholar 

  16. Prausnitz, M. R. (1998) Electroporation, in Electronically Controlled Drug Delivery (Berner, B. and Dinh, S. M., eds.), CRC Press, Boca Raton, FL, pp. 185–214.

    Google Scholar 

  17. Pliquett, U. (1999) Mechanistic studies of molecular transdermal transport due to skin electroporation. Adv. Drug Deliv. Rev. 35, 41–60.

    Article  PubMed  CAS  Google Scholar 

  18. Prausnitz, M. R. (1999) A practical assessment of transdermal drug delivery by skin electroporation. Adv. Drug Deliv. Rev. 35, 61–76.

    Article  PubMed  CAS  Google Scholar 

  19. Vanbever, R. and Preat, V. (1999) In vivo efficacy and safety of skin electroporation. Adv. Drug Deliv. Rev. 35, 78–88.

    Article  Google Scholar 

  20. Gallo, S. A., Oseroff, A. R., Johnson, P. G., and Hui, S. W. (1997) Characterization of electric-pulse-induced permeabilization of porcine skin using surface electrodes. Biophys. J. 72, 2805–2811.

    Article  PubMed  CAS  Google Scholar 

  21. Prausnitz, M. R. (1996) The effects of electric current applied to the skin: A review for transdermal drug delivery. Adv. Drug Deliv. Rev. 18, 395–425.

    Article  CAS  Google Scholar 

  22. Stubbins, W. F. (1986) Essential Electronics. Wiley, New York.

    Google Scholar 

  23. Macdonald, J. R. (1992) Impedance spectroscopy. Ann. Biomed. Eng. 20, 289–305.

    Article  PubMed  CAS  Google Scholar 

  24. Prausnitz, M. R., Lee, C. S., Liu, C. H., Pang, J. C., Singh, T.-P., Langer, R., and Weaver, J. C. (1996) Transdermal transport efficiency during skin electroporation and iontophoresis. J. Controlled Release 38, 205–217.

    Article  CAS  Google Scholar 

  25. Yamamoto, T. and Yamamoto, Y. (1977) Analysis for the change of skin impedance. Med. Biol. Eng. Comput. 15, 219–227.

    Article  PubMed  CAS  Google Scholar 

  26. Leveque, J. L. and Rigal, J. D. (1983) Impedance methods for studying skin moisturization. J. Soc. Cosmet. Chem. 34, 419–428.

    CAS  Google Scholar 

  27. Ackmann, J. J. and Seitz, M. A. (1984) Methods of complex impedance measurements in biologic tissue. Crit. Rev. Biomed. Eng. 2, 281–311.

    Google Scholar 

  28. Burnette, R. R. and DeNuzzio, J. D. (1997) Impedance spectroscopy: Applications to human skin, in Mechanisms of Transdermal Drug Delivery (Potts, P. O. and Guy, R. H., eds.), Marcel Dekker, New York, pp. 215–230.

    Google Scholar 

  29. Jadoul, A., Regnier, V., Doucet, J., Durand, D., and Préat, V. (1997) X-ray scattering analysis of the stratum corneum treated by high voltage pulses. Pharmacol Res. 14, 1275–1277.

    Article  CAS  Google Scholar 

  30. Woolfson, M. M. (1970) X-Ray Crystallography. Cambridge University Press, Cambridge, UK.

    Google Scholar 

  31. Rhodes, G. (1993) Crystallography Made Crystal Clear. Academic Press, San Diego, CA.

    Google Scholar 

  32. White, S. H., Mirejovsky, D., and King, G. I. (1988) Structure of lamellar lipid domains and corneocyte envelopes of muring stratum corneum. An x-ray diffraction study. Biochemistry 27, 3725–3732.

    Article  PubMed  CAS  Google Scholar 

  33. Bouwstra, J. A., Gooris, G. S., de Vries, M. A., van der Spek, J. A., and Bras, W. (1991) Structural investigations of human stratum corneum by small-angle X-ray scattering. J. Invest. Dermatol. 97, 1005–1012.

    Article  PubMed  CAS  Google Scholar 

  34. Garson, J., Doucet, J., Deveque, J. L., and Tsoucaris, G. (1991) Oriented structure in human stratum corneum revealed by X-ray diffraction. J. Invest. Dermatol. 96, 43–49.

    Article  PubMed  CAS  Google Scholar 

  35. Bouwstra, J. A., Gooris, G. S., Salmones-de Vries, M. A., van der Spek, J. A., and Bras, W. (1992) Structure of human stratum corneum as a function of temperature and hydration: A wide-angle X-ray diffraction study. Int. J. Pharm. 84, 205–216.

    Article  CAS  Google Scholar 

  36. Bouwstra, J. A., Gooris, G. S., and White, S. H. (1997) X-ray analysis of the stratum corneum and its lipids, in Mechanisms of Transdermal Drug Delivery (Potts, R. O. and Guy, R. H., eds.), Marcel Dekker, New York, pp. 41–85.

    Google Scholar 

  37. Jadoul, A., Tanajo, H., Préat, V., Spies, F., and Boddé, H. (1998) Electroperturbation of human stratum corneum fine structure by high voltage pulses: A freeze fracture electron microscopy and differential thermal analysis study. J. Invest. Dermatol. Symp. Proc. 3, 153–158.

    CAS  Google Scholar 

  38. Madison, K., Schwartzendruber, D., Wertz, P., and Downing, D. (1987) Presence of intact intercellular lipid lamellae in the upper layers of the stratum corneum. J. Invest. Dermatol. 88, 714–718.

    Article  PubMed  CAS  Google Scholar 

  39. Boddé, H. E., Holman, B., Spies, F., Weerheim, A., Kempenaar, J., Mommaas, M., and Ponec, M. (1990) Freeze-fracture electron microscopy of in vitro recon-structed human epidermis. J. Invest. Dermatol. 95, 108–116.

    Article  PubMed  Google Scholar 

  40. Holman, B. P., Spies, F., and Boddé, H. E. (1990) An optimized freeze-fracture replication procedure for human skin. J. Invest. Dermatol. 94, 332–335.

    Article  PubMed  CAS  Google Scholar 

  41. Elias, P. M. (1991) Epidermal barrier function: Intercellular lamellar lipid structures, origin, composition and metabolism. J. Controlled Release 15, 199–208.

    Article  CAS  Google Scholar 

  42. Tanojo, H., Bouwstra, J. A., Junginger, H. E. and Boddé, H. E. (1994) Subzero thermal analysis of human stratum corneum. Pharmacol. Res. 11, 1610–1616.

    Article  CAS  Google Scholar 

  43. Casal, H. L. and Mantsch, H. H. (1984) Polymorphic phase behavior of phospholipid membranes studied by infrared spectroscopy. Biochim. Biophys. Acta 779, 381–401.

    PubMed  CAS  Google Scholar 

  44. Amey, R. L. and Chapman, D. (1989) Infrared spectroscopic studies of model and natural biomembranes, in Biomembrane Structure and Function (Chapman, D., ed.), Verlag Chemie, Weinheim, Germany.

    Google Scholar 

  45. Mirabella, F. M. (1993) Internal Reflection Spectroscopy: Theory and Practice. Marcel Dekker, New York.

    Google Scholar 

  46. Potts, R. O., Mak, V. H. W., Guy, R. H., and Francoeur, M. L. (1991) Strategies to enhance permeability via stratum coreum lipid pathways. Adv. Lipid Res. 24, 173–210.

    PubMed  CAS  Google Scholar 

  47. Potts, R. O. and Francoeur, M. L. (1993) Infrared spectroscopy of stratum corneum lipids, in Pharmaceutical Skin Penetration Enhancement (Walters, K. A. and Hadgraft, J., eds.), Marcel Dekker, New York.

    Google Scholar 

  48. Naik, A. and Guy, R. H. (1997) Infrared spectroscopic and differential scanning calorimetric investigations of the stratum corneum barrier function, in Mechanisms of Transdermal Drug Delivery (Potts, R. O. and Guy, R. H., eds.), Marcel Dekker, New York, pp. 87–162.

    Google Scholar 

  49. Bommannan, D., Potts, R. O., and Guy, R. H. (1990) Examination of stratum corneum barrier function in vivo by infrared spectroscopy. J. Invest. Dermatol. 95, 403–408.

    Article  PubMed  CAS  Google Scholar 

  50. Williams, A. C., Barry, B. W., Edwards, H. G., and Farwell, D. W. (1993) A critical comparison of some Raman spectroscopic techniques for studies of human stratum corneum. Pharmacol. Res. 10, 1642–1647.

    Article  CAS  Google Scholar 

  51. Jadoul, A. (1997) Skin Perturbations Induced by Iontophoresis and Electroporation: Enhancement in Drug Transport and Integrity of the Stratum Corneum. Ph.D. Thesis. Université catholique de Louvain, Brussels, Belgium.

    Google Scholar 

  52. Donovan, J. W. (1984) Scanning calorimetry of complex biological structures. Trends in Biol. Sci. 9, 340–344.

    Article  CAS  Google Scholar 

  53. Lewis, R. N. A. H. and McElhaney, R. N. (1992) The mesomorphic phase behavior of lipid bilayers, in The Structure of Biological Membranes (Yeagle, P., ed.), CRC Press, Boca Raton, FL.

    Google Scholar 

  54. Golden, G. M., Guzek, D. B., Harris, R. R., McKie, J. E., and Potts, R. O. (1986) Lipid thermotropic transitions in human stratum corneum. J. Invest. Dermatol. 86, 255–259.

    Article  PubMed  CAS  Google Scholar 

  55. Vanbever, R. (1997) Skin Electroporation for Transdermal Drug Delivery. Ph.D. Thesis. Université catholique de Louvain, Brussels, Belgium.

    Google Scholar 

  56. Pliquett, U., Zewert, T. E., Chen, T., Langer, R., and Weaver, J. C. (1996) Imaging of fluorescent molecule and small ion transport through human stratum corneum during high voltage pulsing: Localized transport regions are involved. Biophys. Chem. 58, 185–204.

    Article  PubMed  CAS  Google Scholar 

  57. Prausnitz, M. R., Gimm, J. A., Guy, R. H., Langer, R., Weaver, J. C., and Cullander, C. (1996) Imaging of transport pathways across human stratum corneum during high-voltage and low-voltage electrical exposures. J. Pharm. Sci. 85, 1363–1370.

    Article  PubMed  CAS  Google Scholar 

  58. Cullander, C. and Guy, R. H. (1991) Sites of iontophoretic current flow into the skin: Identification and characterization with the vibrating probe electrode. J. Invest. Dermatol. 97, 55–64.

    Article  PubMed  CAS  Google Scholar 

  59. Cullander, C. and Guy, R. H. (1992) Visualization of iontophoretic pathways with confocal microscopy and the vibrating probe electrode. Solid State Ionics 53-56, 197–206.

    Article  CAS  Google Scholar 

  60. Scott, E. R., White, H. S., and Phipps, J. B. (1992) Direct imaging of ionic pathways in stratum corneum using scanning electrochemical microscopy. Solid State Ionics 53-56, 176–183.

    Article  CAS  Google Scholar 

  61. Scott, E. R., Laplaza, A. I., White, H. S., and Phipps, J. B. (1993) Transport of ionic species in skin: contribution of pores to the overall skin conductance. Pharmacol. Res. 10, 1699–1709.

    Article  CAS  Google Scholar 

  62. Turner, N. G. and Nonato, L. B. (1997) Visualization of stratum corneum and transdermal permeation pathways, in Mechanisms of Transdermal Drug Delivery (Potts, R. O. and Guy, R. H., eds.), Marcel Dekker, New York, pp. 1–40.

    Google Scholar 

  63. Haugland, R. P. (1997) Handbook of Fluorescent Probes and Research Chemicals. Molecular Probes, Eugene, OR.

    Google Scholar 

  64. Pawley, J. B. (1990) Handbook of Biological Confocal Microscopy. Plenum, New York.

    Google Scholar 

  65. Wilson, T. (1990) Confocal Microscopy. Academic Press, New York.

    Google Scholar 

  66. Grimnes, S. (1984) Pathways of ionic flow through human skin in vivo. Acta Derm. Venereol. 64, 93–98.

    PubMed  CAS  Google Scholar 

  67. Burnette, R. R. and Ongpipattanakul, B. (1988) Characterization of the pore transport properties and tissue alteration of excised human skin during iontophoresis. J. Pharm. Sci. 77, 132–137.

    Article  PubMed  CAS  Google Scholar 

  68. Monteiro-Riviere, N. A., Inman, A. O., and Riviere, J. E. (1994) Identification of the pathway of iontophoretic drug delivery: Light and ultrastructural studies using mercuric chloride in pigs. Pharmacol. Res. 11, 251–256.

    Article  CAS  Google Scholar 

  69. Rutherford, T. and Black, J. G. (1969) The use of autoradiography to study the localization of germicides in skin. Br. J. Dermatol. 81(Suppl. 4), 75–87.

    Article  CAS  Google Scholar 

  70. Bidman, H. J., Pitts, J. D., Solomon, H. F., Bondi, J. V., and Stumpf, W. E. (1990) Estradiol distribution and penetration in rat skin after topical application, studies by high resolution autoradiography. Histochem. J. 95, 43–54.

    Article  Google Scholar 

  71. Simonetti, O., Hoogstraate, A. J., Bialik, W., Kempenaar, J. A., Schrijvers, A. H., Bodde, H. E., and Ponec, M. (1995) Visualization of diffusion pathways across the stratum corneum of native and in-vitro-reconstructed epidermis by confocal laser scanning microscopy. Arch. Dermatol. Res. 287, 465–473.

    Article  PubMed  CAS  Google Scholar 

  72. Turner, N. G. and Guy, R. H. (1997) Iontophoretic transport pathways: Dependence on penetrant physicochemical properties. J. Pharm. Sci. 86, 1385–1389.

    Article  PubMed  CAS  Google Scholar 

  73. Squier, C. and Hopps, R. (1976) A study of the permeability barrier in epidermis and oral epithelium using horseradish peroxidase as a tracer in vitro. Br. J. Dermatol. 95, 123–129.

    Article  PubMed  CAS  Google Scholar 

  74. Menon, G. K., Bommannan, D. B., and Elias, P. M. (1994) High-frequency sonophoresis: permeation pathways and structural basis for enhanced permeability. Skin Pharmacol. 7, 130–139.

    Article  PubMed  CAS  Google Scholar 

  75. Ogiso, T., Iwaki, M., Bechako, K., and Tsutsum, Y. (1992) Enhancement of percutaneous absorption by laurocapram. J. Pharm. Sci. 81, 762-767.

    Google Scholar 

  76. Elias, P. M. and Friend, D. S. (1975) The permeability barrier in mammalian epidermis. J. Cell Biol. 65, 180–191.

    Article  PubMed  CAS  Google Scholar 

  77. Hofland, H. E., Bouwstra, J. A., Boddé, H. E., Spies, F., and Junginger, H. E. (1995) Interactions between liposomes and human stratum corneum in vitro: Freeze fracture electron microscopical visualization and small angle x-ray scattering studies. Br. J. Dermatol. 132, 853–866.

    Article  PubMed  CAS  Google Scholar 

  78. Nemanic, M. K. and Elias, P. M. (1980) In situ precipitation: A novel cytochemical technique for visualization of permeability pathways in mammalian stratum corneum. J. Histochem. Cytochem. 28, 573–578.

    PubMed  CAS  Google Scholar 

  79. Boddé, H. E., van den Brink, I., Koerten, H. K. and de Haan, F. H. N. (1991) Visualization of in vitro percutaneous penetration of mercuric chloride: Transport through intercellular space versus cellular uptake through desmosomes. J. Controlled Release 15, 227–236.

    Article  Google Scholar 

  80. King, C., Moore, N., Marks, R. and Nicholls, S. (1978) Preliminary studies into percorneal penetration and elemental content of the stratum corneum using x-ray microanalysis. Arch. Dermatol. Res. 263, 257–265.

    Article  PubMed  CAS  Google Scholar 

  81. Agner, T. (1992) Noninvasive measuring methods for the investigation of irritant patch test reactions: A study of patients with hand eczema, atopic dermatitis and controls. Acta Derm. Venereol. (Stockh.) Suppl. 173, 1–26.

    CAS  Google Scholar 

  82. Serup, J. and Jemec, G. B. E. (1995) Handbook of Non-Invasive Methods and the Skin. CRC Press, Boca Raton, FL.

    Google Scholar 

  83. Berardesca, E. and Maibach, H. I. (1988) Bioengineering and the patch test. Contact Dermatitis 18, 3–9.

    Article  PubMed  CAS  Google Scholar 

  84. Elsner, P., Wilhelm, D., and Maibach, H. I. (1990) Multiple parameter assessement of vulvar irritant contact dermatitis. Contact Dermatitis 23, 20–26.

    Article  PubMed  CAS  Google Scholar 

  85. Vanbever, R., Fouchard, D., Jadoul, A., De Morre, N., Preat, V., and Marty, J.-P. (1998) In vivo non-invasive evaluation of hairless rat skin after high-voltage pulse exposure. Skin Pharmacol. Appl. Skin Physiol. 11, 23–24.

    PubMed  CAS  Google Scholar 

  86. Ledger, P. W. (1992) Skin biological issues in electrically enhanced transdermal delivery. Adv. Drug Deliv. Rev. 9, 289–307.

    Article  CAS  Google Scholar 

  87. Westerhof, W. (1995) CIE Colorimetry, in Handbook of Non-Invasive Methods and the Skin (Serup, J. and Jemec, G. B. E., eds.), CRC Press, Boca Raton, FL, pp. 385–397.

    Google Scholar 

  88. Serup, J. and Agner, T. (1990) Colorimetric quantification of erythema: A comparison of two colorimeters (Lange Micro Color and Minolta Chroma Meter CR-200) with a clinical scoring scheme and laser-Doppler flowmetry. Clin. Exp. Dermatol. 15, 267–272.

    Article  PubMed  CAS  Google Scholar 

  89. Bjerring, P. (1995) Spectrophotometric characterization of skin pigments and skin color, in Handbook of Non-Invasive Methods and the Skin (Serup, J. and Jemec, G. B. E., eds.), CRC Press, Boca Raton, FL, pp. 373–376.

    Google Scholar 

  90. Takiwaki, H. and Serup, J. (1995) Measurement of erythema and melanin indices, in Handbook of Non-Invasive Methods and the Skin (Serup, J. and Jemec, G. B. E., eds.), CRC Press, Boca Raton, FL, pp. 377–384.

    Google Scholar 

  91. Eun, H. C. (1995) Evaluation of skin blood flow by laser doppler flowmetry. Clin. Dermatol. 13, 337–347.

    Article  PubMed  CAS  Google Scholar 

  92. Bircher, A., De Boer, E. M., Agner, T., Wahlberg, J. E., and Serup, J. (1994) Guidelines for measurement of cutaneous blood flow by laser Doppler flowmetry. Contact Dermatitis 30, 65–72.

    Article  PubMed  CAS  Google Scholar 

  93. Belcaro, G. and Nicolaides, A. N. (1995) Laser-Doppler flowmetry: Principles of technology and clinical applications, in Handbook of Non-Invasive Methods and the Skin (Serup, J. and Jemec, G. B. E., eds.), CRC Press, Boca Raton, FL, pp. 405–410.

    Google Scholar 

  94. Bircher, A. J. (1995) Laser Doppler measurement of skin blood flux: Variation and validation, in Handbook of Non-Invasive Methods and the Skin (Serup, J. and Jemec, G. B. E., eds.), CRC Press, Boca Raton, FL, pp. 399–403.

    Google Scholar 

  95. Pinnagoda, J. and Tupker, R. A. (1995) Measurement of the transepidermal water loss, in Handbook of Non-Invasive Methods and the Skin (Serup, J. and Jemec, G. B. E., eds.), CRC Press, Boca Raton, FL, pp. 173–178.

    Google Scholar 

  96. Pinnagoda, J., Tupker, R. A., Agner, T., and Serup, J. (1990) Guidelines for transepidermal water loss (TEWL) measurement. Contact Dermatitis 22, 164–178.

    Article  PubMed  CAS  Google Scholar 

  97. Barel, A. O. and Clarys, P. (1995) Measurement of epidermal capacitance, in Handbook of Non-Invasive Methods and the Skin (Serup, J. and Jemec, G. B. E., eds.), CRC Press, Boca Raton, FL, pp. 165–170.

    Google Scholar 

  98. Tagami, H. (1995) Measurement of electrical conductance and impedance, in Handbook of Non-Invasive Methods and the Skin (Serup, J. and Jemec, G. B. E., eds.), CRC Press, Boca Raton, FL, pp. 159–164.

    Google Scholar 

  99. Serup, J., Keiding, J., Fullerton, A., Gniadecka, M., and Gniadecki, R. (1995) High-frequency ultrasound examination of skin: Introduction and guide, in Handbook of Non-Invasive Methods and the Skin (Serup, J. and Jemec, G. B. E., eds.), CRC Press, Boca Raton, FL, pp. 239–256.

    Google Scholar 

  100. Agner, T. (1995) Ultrasound A-mode measurement of skin thickness, in Handbook of Non-Invasive Methods and the Skin (Serup, J. and Jemec, G. B. E., eds.), CRC Press, Boca Raton, FL, pp. 289–292.

    Google Scholar 

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Author information

Authors and Affiliations

  1. Schools of Chemical Engineering and Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA

    Mark R. Prausnitz

  2. University of Bielefeld, Bielefeld, Germany

    Uwe Pliquett (Faculty of Chemistry/Physical Chemistry)

  3. Department of Pharmaceutical Technology, School of Pharmacy, Catholic University of Louvain, Brussels, Belgium

    Rita Vanbever

Authors
  1. Mark R. Prausnitz
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  2. Uwe Pliquett
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  3. Rita Vanbever
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Editor information

Editors and Affiliations

  1. University of South Florida College of Medicine, USA

    Mark J. Jaroszeski PhD  & Richard Heller PhD  & 

  2. University of South Florida College of Engineering, USA

    Richard Gilbert PhD

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Prausnitz, M.R., Pliquett, U., Vanbever, R. (2000). Mechanistic Studies of Skin Electroporation Using Biophysical Methods. In: Jaroszeski, M.J., Heller, R., Gilbert, R. (eds) Electrochemotherapy, Electrogenetherapy, and Transdermal Drug Delivery. Methods in Molecular Medicine, vol 37. Humana Press. https://doi.org/10.1385/1-59259-080-2:213

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  • DOI: https://doi.org/10.1385/1-59259-080-2:213

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-606-2

  • Online ISBN: 978-1-59259-080-3

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