Abstract
Hepatocellular carcinoma (HCC) is one of the most common causes of cancer death worldwide and is especially prevalent in certain areas of Africa and Asia. The most important etiological factor is infection with the hepatitis B or C virus. Treatment is generally unsatisfactory as the majority of patients are not suitable for surgical resection and chemotherapy is not particularly effective.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Ding, S.-F., Habib, N. A., Dooley, J., Wood, C., Bowles, L., and Delhanty, J. D. A. (1991) Loss of constitutional heterozygosity on chromosome 5q in hepatocellular carcinoma without cirrhosis. Br. J. Cancer. 64, 1083–1087.
Mitry, R. R., Sarraf, C. E., Wu, C.-G., Pignatelli, M., and Habib, N. A. (1997) Wild-type p53 induces apoptosis in Hep3B through up-regulation of Bax expression. Lab. Invest. 77, 369–378.
Anderson, S. C., Engler, H., Johnson, D. E. et al. (1995) p53 gene therapy in a rat model of hepatocellular carcinoma. Cancer Gene Ther. 2, 335–336.
Lowe, S. W., Ruler, H. E., Jacks, T., and Housman, D. E. (1993) p53-dependent apoptosis modulates the cytotoxicity of anticancer agents. Cell. 74, 957–967.
Slack, R. S., Belliveau, D. J., Rosenberg, M., et al. (1996) Adenovirus mediated gene transfer of the tumor suppressor, p53, induces apoptosis in postmitotic neurons. J. Cell Biol. 135, 1085–1096.
Nielsen, L. L. and Maneval, D. C. (1998) p53 tumor suppressor gene therapy for cancer. Cancer Gene Ther. 5, 52–63.
Wolff, J. A. (1999) Naked DNA gene transfer in mammalian cells, in: The Development of Human Gene Therapy (Friedmann, T., ed.) Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
Rill, D. R., Moen, R. C., Buschle, M., Bartholomew, C., Foreman, N. K., Mirro, J., et al. (1992) An approach for the analysis of relapse and marrow reconstitution after autologous marrow transplantation using retrovirus-mediated gene transfer. Blood. 79(10), 2694–2700.
Habib, N. A., Ding, S.-F., El-Masry, R., Dalla Serra, G., Mikhail, N. E., Izzi, G., et al. (1995) Contrasting effects of direct p53 DNA injection in primary and secondary liver tumors. Tumor Targeting 1, 295–998.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2000 Humana Press Inc., Totowa, NJ
About this protocol
Cite this protocol
Mitry, R.R., Kelly, M.D., Zhao, J., Negishi, S., Mansour, M.R., Habib, N.A. (2000). p53 Plasmid Preparation and Techniques for Analysis of Gene Transfer and Expression. In: Habib, N.A. (eds) Hepatocellular Carcinoma. Methods in Molecular Medicine™, vol 45. Humana Press. https://doi.org/10.1385/1-59259-079-9:207
Download citation
DOI: https://doi.org/10.1385/1-59259-079-9:207
Publisher Name: Humana Press
Print ISBN: 978-0-89603-785-4
Online ISBN: 978-1-59259-079-7
eBook Packages: Springer Protocols