Abstract
Hepatocellular carcinoma (HCC) is one of the most common human malignancies in the world and it is especially prevalent in Asia and Africa (1). Some tumors are suitable for resection, however, there is a very high rate of recurrence (2). Infection with hepatitis B or C virus, alcoholic cirrhosis, and ingestion of aflatoxin B1-contaminated food are important risk factors for HCC (3,4), but the exact molecular mechanism of tumorigenesis is still unclear.
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References
Di Bisceglie, A. M., Rustgi, V. K., Hoofnagle, J. H., Dusheiko, G. M., and Lotze, M. T. (1988) Hepatocellular carcinoma. Ann. Intern. Med. 108, 390–401.
Jwo, S-C., Chiu, J-H., Chau, G-Y., Loong, C. C., and Lui, W-Y. (1992) Risk factors linked to tumor recurrence of human hepatocellular carcinoma after hepatic resection. Hepatology. 16, 1367–1371.
Harris, C. C. (1990) Hepatocellular carcinogenesis: recent advances and speculations. Cancer Cells. 2, 146–148.
Nalpas, B., Driss, F., Pol, S., Hamelin, B., Housset, C., Brechot, C., et al. (1991) Association between HCV and HVB infection in hepatocellular carcinoma and alcoholic liver disease. J. Hepatol. 12, 70–74.
Wang, H. P. and Rogler. (1988) Deletions in human chromosome arms 11p and 13q in primary hepatocellular carcinomas. Cytogenet. Cell. Genet. 48, 72–78.
Buetow, K. H., Murray, J. C., Israel, J. L., London, W. T., Smith, M. Kew, M. et al. (1989) Loss of heterozygosity suggests tumor suppressor gene responsible for primary hepatocellular carcinoma. Proc. Natl. Acad. Sci. USA 86, 8852–8856.
Zhang, W., Hirohashi, S., Tsuda, H., Shimosato, Y., Yokota, J., Terada, M., et al. (1990) Frequent loss of heterozygosity on chromosome 16 and 4 in human hepatocellular carcinoma. Jpn. J. Cancer. Res. 81, 108–111.
Fijimori, M., Mitunobu, M., Ishikawa, T., and Nakamura, Y. (1991) Allelotype study of primary hepatocellular carcinoma. Cancer Res. 51, 89–93.
Simon, D., Knowles, B. B., and Weith, A. (1991) Abnormalities of chromosome 1 and loss of heterozygosity on 1p in primary hepatomas. Oncogene. 6, 765–770.
Murakami, Y., Hayashi, K., Hirohashi, S., and Sekiya, T. (1991) Aberrations of the tumor suppressor p53 and retinoblastoma genes in human hepatocellular carcinomas. Cancer Res. 51, 5520–5525.
Tsuda, H., Zhang, W., Shimosato, Y., Yokota, J., Terada, M., Sugimura, T., et al. (1990) allele loss on chromosome 16 associated with progression of human hepatocellular carcinoma. Proc. Nat. Acad. Sci. USA 87, 6791–6794.
Babrow, M. N., Harris, T. D., Shaughnessy, K. J., and Litt, G. J. (1989) Catalyzed reporter deposition, a novel method of signal amplification: application to immunoassays. J. Immunol. Methods. 125, 279–285.
Adams, J. C. (1992) Biotin amplification of biotin and horseradish peroxidase signals in histochemical stains. J. Histochem. Cytochem. 40, 1457–1463.
Plenat, F., Picard, E., Antunes, L., Vignaud, J. M., Marie, B., Chalabreysse, P., et al. (1997) Amplification of immunologic reactions using catalytic deposition at the reaction sites of tyramine derivatives. A decisive gain in sensitivity in immnohistochemistry and in situ hybridization. Ann. Pathol. 17, 17–23.
Elias, J. M., Gown, A. M., Nakamura, R. M., Wilbur, D. C., Herman, G. E., Jaffe, E. S., et al. (1989) Special report: quality control in immunohistochemistry. Am. J. Clin. Pathol. 92, 836–843.
Shi, S. R., Key, M. C., and Kalra, K. L. (1991) Antigen retrieval in formalin-fihxed, paraffin-embedded tissues: an enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections. J. Histochem. Cytochem. 39, 741–748.
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© 2000 Humana Press Inc., Totowa, NJ
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Naka, T., Kaneko, T., Kaibara, N. (2000). Measurement of Protein Expression of p53, p21WAF1, and Rb in Patients with Surgically Treated Hepatocellular Carcinoma by Using Catalyzed Signal-Amplification System. In: Habib, N.A. (eds) Hepatocellular Carcinoma. Methods in Molecular Medicine™, vol 45. Humana Press. https://doi.org/10.1385/1-59259-079-9:167
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DOI: https://doi.org/10.1385/1-59259-079-9:167
Publisher Name: Humana Press
Print ISBN: 978-0-89603-785-4
Online ISBN: 978-1-59259-079-7
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