Abstract
With an estimated annual incidence of between 250,000 and 1 million cases worldwide, hepatocellular carcinoma (HCC) is one of the most frequent malignancies in humans and occurs mainly in areas of Asia and Africa (1,2). Early diagnosis is critical for patients with HCC, because the 5-yr survival rate is significantly improved for patients with small, subclinical HCC after surgical resection (3). Therefore, identification of gene products exclusively or abundantly expressed in HCC tissue may yield novel tumor markers that are sensitive and specific to HCC. Moreover, it could lead to the early detection and further efficient targeting of tumor, and eventually to cure the disease.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Di Bisceglie, A. M., Rustgi, V. K., Hoofnagle, J. H., Dusheiko, G. M., and Lotze, M. T. (1988) NIH conference. Hepatocellular carcinoma. Ann. Intern. Med. 108, 390–401.
Rosenberg, S. A., Longo, D. L., and Lotze, M. T. (1989) Principles and Applications of Biologic Therapy, in Cancer: Principles and Practice of Oncology, (DeVita, V. T., Jr., Hellman, S. and Rosenberg, S. A., eds.) JB Lippincott, Philadelphia, pp. 301–348.
Tang, Z. Y., Yu, Y. Q., Zhou, X. D., Ma, Z. C., Yang, R., Lu, J. Z., Lin, Z. Y., and Yang, B. H. (1989) Surgery of small hepatocellular carcinoma. Analysis of 144 cases [see comments]. Cancer. 64, 536–541.
Land, H., Parada, L. F., and Weinberg, R. A. (1983) Cellular oncogenes and multistep carcinogenesis. Science. 222, 771–778.
Vogelstein, B., Fearon, E. R., Baker, S. J., Nigro, J. M., Kern, S. E., Hamilton, S. R., et al. (1989) Genetic alterations accumulate during colorectal tumorigenesis, in Current Communications in Molecular Biology. Recessive Oncogenes and Tumor Suppression, (Cavenee, W., Hastle, N., and Stanbridge, E. J. eds.) Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, pp. 73–80.
Sukumar, S. (1990) An experimental analysis of cancer: role of ras oncogenes in multistep carcinogenesis. Cancer Cells. 2, 199–204.
Elmore, L. W. and Harris, C. C. (1998) Hepatocellular carcinoma, in The Genetic Basis of Human Cancer, (Vogelstein, B. and Kinzler, K. W. eds.) McGraw-Hill, New York, pp. 682–689.
Bishop, J. M. (1988) The molecular genetics of cancer: 1988. Leukemia. 2, 199–208.
Rodenhuis, S., Slebos, R. J., Boot, A. J., Evers, S. G., Mooi, W. J., Wagenaar, S. S., et al. (1988) Incidence and possible clinical significance of K-ras oncogene activation in adenocarcinoma of the human lung. Cancer Res. 48, 5738–5741.
Pillai, R. (1992) Oncogenes and oncoproteins as tumor markers. Eur. J. Surg. Oncol. 18, 417–424.
Farber, E. (1984) The multistep nature of cancer development. Cancer Res. 44, 4217–4223.
Farber, E. (1984) Chemical carcinogenesis: a current biological perspective. Carcinogenesis. 5, 1–5.
Wang, Z. and Brown, D. D. (1991) A gene expression screen. Proc. Natl. Acad. Sci USA 88, 11,505–11,509.
Wu, C. G., Groenink, M., Bosma, A., Reitsma, P. H., van Deventer, S. J., and Chamuleau, R. A. (1997) Rat ferritin-H: cDNA cloning, differential expression and localization during hepatocarcinogenesis. Carcinogenesis. 18, 47–52.
Chomczynski, P. and Sacchi, N. (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162, 156–159.
Liang, P. and Pardee, A. B. (1992) Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction. Science. 257, 967–971.
Bertioli, D. J., Schlichter, U. H., Adams, M. J., Burrows, P. R., Steinbiss, H. H., and Antoniw, J. F. (1995) An analysis of differential display shows a strong bias towards high copy number mRNAs. Nucleic Acids Res. 23, 4520–4523.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2000 Humana Press Inc., Totowa, NJ
About this protocol
Cite this protocol
Wu, CG. (2000). Isolation of Novel Markers for Hepatocellular Carcinoma by a Subtraction-Enhanced Display Technique. In: Habib, N.A. (eds) Hepatocellular Carcinoma. Methods in Molecular Medicine™, vol 45. Humana Press. https://doi.org/10.1385/1-59259-079-9:157
Download citation
DOI: https://doi.org/10.1385/1-59259-079-9:157
Publisher Name: Humana Press
Print ISBN: 978-0-89603-785-4
Online ISBN: 978-1-59259-079-7
eBook Packages: Springer Protocols