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Electron Cryomicroscopy and Computer Image Processing Techniques

Use in Structure-Function Studies of Rotavirus
  • B. V. Venkataram Prasad
  • Mary K. Estes
Part of the Methods in Molecular Medicine™ book series (MIMM, volume 34)

Abstract

Rotavirus (RV), a double-stranded (ds)RNA virus in the family Reoviridae, is a complex, relatively large (diameter, including spikes = 1000 Å), nonenveloped icosahedral virus. Once RV was recognized as a major human pathogen, it was extensively studied using modern molecular genetic and biological techniques, as discussed elsewhere in this book. These studies provided basic information about gene-coding assignments, protein processing, genome expression and replication, viral morphogenesis, and pathogenesis (1). In addition, molecular epidemiological studies, coupled with the characterization of neutralizing monoclonal antibodies (MAbs) and sequencing of the genes that encode the neutralizing antigens, provided an understanding at the molecular level of the antigenic and genetic variability of the RVs.

Keywords

dsRNA Virus Icosahedral Symmetry Icosahedral Virus Computer Image Processing Aqueous Channel 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 2000

Authors and Affiliations

  • B. V. Venkataram Prasad
    • 1
  • Mary K. Estes
    • 2
  1. 1.Verna and Marrs Department of Biochemistry and W. M. Keck Center for Computational BiologyBaylor College of MedicineHouston
  2. 2.Division of Molecular VirologyBaylor College of MedicineHouston

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