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Diagnosis of Penicillin Resistance by PCR-RFLP

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Antibiotic Resistence

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 48))

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Abstract

Streptococcus pneumoniae is an important human pathogen causing a wide spectrum of disease including pneumonia, otitis media, bacteraemia, and meningitis. It is a significant cause of morbidity and mortality worldwide and now penicillin resistance is becoming an ever increasing problem (1-2). Initially, all S. pneumoniae isolates were exquisitely sensitive to penicillin and thus it was the drug of choice. However, the increase in resistance to penicillin seen in S. pneumoniae throughout the world has complicated treatment protocols. Penicillin resistance in S. pneumoniae also leads to some degree of cross resistance to other β-lactams, including the third generation cephalosporins and the carbapenems.

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Author information

Authors and Affiliations

  1. Department of Medical Microbiology, Royal Free and University College Medical School, Royal Free Campus, London, UK

    Gail C. Whiting

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  1. Gail C. Whiting
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Editors and Affiliations

  1. Department of Medical Microbiology, Royal Free and University College Medical School, University College London, London, UK

    Stephen H. Gillespie

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© 2001 The Humana Press Inc., Totowa, NJ

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Whiting, G.C. (2001). Diagnosis of Penicillin Resistance by PCR-RFLP. In: Gillespie, S.H. (eds) Antibiotic Resistence. Methods in Molecular Medicine™, vol 48. Humana Press. https://doi.org/10.1385/1-59259-077-2:77

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  • DOI: https://doi.org/10.1385/1-59259-077-2:77

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-777-9

  • Online ISBN: 978-1-59259-077-3

  • eBook Packages: Springer Protocols

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