Abstract
The affinity and kinetics of antibody-antigen interactions are increasingly realized to be important parameters in determining the usefulness of antibodies in both in vivo and in vitro settings (1). As discussed in Chapter 1, the affinity of an antibody (given by the association or dissociation equilibrium constants; Ka and Kd) gives information about how much antibody will be bound to the antigen at equilibrium, or how much antibody will be required for a particular proportion of the antigen to be bound. The kinetics of the interaction (given by the association and dissociation rate constants; kass and kdiss) tell how rapidly the antibody-antigen complex is formed and, once formed, how rapidly it dissociates.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
George, A. J. T., Rashid, M., and Gallop, J. L. (1997) Kinetics of biomolecular interactions. Expert Opin. Ther. Patents7, 947–963.
Buckle, P. E., Davies, R. J., Kinning, T., Yeung, D., Edwards, P. R., Pollard-Knight, D., and Lowe, C. R. (1993) The resonant mirror: a novel optical biosensor for direct sensing of biomolecular interactions part II: applications. Biosensors Biolectr.8, 355–368.
Cush, R., Cronin, J. M., Stewart, W. J., Maule, C. H., Molloy, J., and Goddard, N. J. (1993) The resonant mirror: a novel optical biosensor for direct sensing of biomolecular interactions part I: principles of operation and associated instrumentation. Biosensors Biolectr.8, 347–353.
George, A. J. T., French, R. R., and Glennie, M. J. (1995) Measurement of kinetic binding constants of a panel of anti-saporin antibodies using a resonant mirror biosensor. J. Immunol. Methods183, 51–63.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2000 Humana Press Inc.
About this protocol
Cite this protocol
George, A.J.T. (2000). Use of Biosensors to Measure the Kinetics of Antibody-Antigen Interactions. In: George, A.J.T., Urch, C.E. (eds) Diagnostic and Therapeutic Antibodies. Methods in Molecular Medicine, vol 40. Humana, Totowa, NJ. https://doi.org/10.1385/1-59259-076-4:363
Download citation
DOI: https://doi.org/10.1385/1-59259-076-4:363
Publisher Name: Humana, Totowa, NJ
Print ISBN: 978-0-89603-798-4
Online ISBN: 978-1-59259-076-6
eBook Packages: Springer Protocols