Preparation of Recombinant RNase Single-Chain Antibody Fusion Proteins

Part of the Methods in Molecular Medicine™ book series (MIMM, volume 25)


Selective cytotoxicity is an important goal of specific drug targeting. Toward this end, toxins isolated primarily from higher plants and bacteria have been coupled to monoclonal antibodies (MAbs) and evaluated for their clinical efficacy in cancer, AIDS, and immunological diseases (1,2). Immune responses against murine monoclonal antibodies MAbs (3,4) and antitoxin antibodies have been detected in both animals and humans treated with immunotoxins (ITs) (5, 6, 7) and present a major obstacle to the successful application of this technology. Although development of humanized antibodies have alleviated some of these effects (8, and references therein), the toxins themselves remain a problem. Consequently, the identification of human proteins to be used as components of immunoconjugates is highly desirable


Inclusion Body Polymerase Chain Reaction Reaction Luria Broth Sense Direction Fast Protein Liquid Chromatography 
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Copyright information

© Humana Press Inc. 2000

Authors and Affiliations

  1. 1.Intramural Research Support Program, SAIC FrederickNational Cancer Institute-Frederick Cancer Research and Development CenterFrederick
  2. 2.Laboratory of Biochemical Physiology, Division of Basic ScienceNational Cancer Institute-Frederick Cancer Research and Development CenterFrederick

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