Advertisement

Folate as a Targeting Device for Proteins Utilizing Folate Receptor-Mediated Endocytosis

Protocol
Part of the Methods in Molecular Medicine™ book series (MIMM, volume 25)

Abstract

Folic acid (M r = 441, Fig. 1 ) is a vitamin essential for de novo nucleotide synthesis and one-carbon metabolism. The ability to acquire folate, therefore, is important to the viability of proliferating cells.
Fig. 1.

Folic acid.

Keywords

Folate Receptor Reduce Folate Carrier Protein Conjugate Desalting Column Free Folate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Antony, A. C. (1992) The biological chemistry of folate receptors. Blood 79, 2807–2820.PubMedGoogle Scholar
  2. 2.
    Kamen, B. A., Wang, M. T., Streckfuss, A. J., Preyea, X., and Anderson, R. G. (1988) Delivery of folates to the cytoplasm of MA 104 cells is mediated by a surface receptor that recycles. J. Biol. Chem. 263, 13,602–13,609.PubMedGoogle Scholar
  3. 3.
    Ross, J. F., Chaudhuri, P. K., and Ratnam, M. (1994) Differential regulation of folate receptor isoforms in normal and malignant tissues in vivo and in established cell lines. Cancer 73, 2432–2443.CrossRefPubMedGoogle Scholar
  4. 4.
    Weitman, S. D., Lark, R. H., Coney, L. R., Fort, D. W., Frasca, V., Zurawski, V. R., Jr., and Kamen, B. A. (1992) Distribution of the folate receptor GP38 in normal and malignant cell lines and tissues. Cancer Res. 52, 3396–3401.PubMedGoogle Scholar
  5. 5.
    Garin-Chesa, P., Campbell, I., Saigo, P. E., Lewis, J. L. J., Old, L. J., and Rettig, W. J. (1993) Trophoblast and ovarian cancer antigen LK26. Sensitivity and specificity in immunopathology and molecular identification as a folate-binding protein. Am. J. Pathol. 142, 557–567.PubMedGoogle Scholar
  6. 6.
    Rothberg, K. G., Ying, Y. S., Kolhouse, J. F., Kamen, B. A., and Anderson, R. G. (1990) The glycophospholipid-linked folate receptor internalizes folate without entering the clathrin-coated pit edocytic pathway. J. Cell Biol. 110, 637–649.CrossRefPubMedGoogle Scholar
  7. 7.
    Rijnboutt, S., Jansen, G., Posthuma, G., Hynes, J. B., Schornagel, J. H., and Strous, G. J. (1996) Endocytosis of GPI-linked membrane folate receptor-α. J. Cell Biol. 132, 35–47.CrossRefPubMedGoogle Scholar
  8. 8.
    Leamon, C. P. and Low, P. S. (1991) Delivery of macromolecules into living cells: a method that exploits folate receptor endocytosis. Proc. Natl. Acad. Sci. USA 88, 5572–5576.CrossRefPubMedGoogle Scholar
  9. 9.
    Lee, R. J. and Low, P. S. (1994) Delivery of liposomes into cultured KB cells via folate receptor-mediated endocytosis. J. Biol. Chem. 269, 3198–3204.PubMedGoogle Scholar
  10. 10.
    Wang, S., Lee, R. J., Mathias, C. J., Green, M. A., and Low, P. S. (1996) Synthesis, purification, and tumor cell uptake of Gallium-67-deferoxamine-folate, a potential radiopharmaceutical for tumor imaging. Bioconj. Chem. 7, 56–62.CrossRefGoogle Scholar
  11. 11.
    Leamon, C. P. and Low, P. S. (1992) Cytotoxicity of momordin-folate conjugates in cultured human cells. J. Biol. Chem. 267, 24,966–24,971.PubMedGoogle Scholar
  12. 12.
    Leamon, C. P. and Low, P. S. (1993) Membrane folate-binding proteins are responsible for folate-protein conjugate endocytosis into cultured cells. Biochem. J. 291, 855–860.PubMedGoogle Scholar
  13. 13.
    Leamon, C. P., Pastan, I., and Low, P. S. (1993) Cytotoxicity of folate-pseudonomas exotoxin conjugates towards tumor cells. Contribution of translo-cation domain. J. Biol. Chem. 25, 24,847–24,854.Google Scholar
  14. 14.
    Lee, R. J. and Low, P. S. (1994) Folate-mediated tumor cell targeting of lipo-some-entrapped doxorubicin in vitro. Biochim. Biophys. Acta 1233, 134–144.Google Scholar
  15. 15.
    Wang, S., Luo, J., Lantrip, D. A., Waters, D. J., Mathias, C. J., Green, M. A., Fuchs, P. L., and Low, P. S. (1997) Design and Synthesis of 111In-DTPA-Folate for Use as a Tumor-Targeted Radiopharmaceutical. Bioconj. Chem., submitted.Google Scholar
  16. 16.
    Mathias, C. J., Waters, D. J., Wang, S., Low, P. S., and Green, M. A. (1997) Indium-111 labeled Indium(III)-DTPA-folate as a radiopharmaceutical for targeting tumor-associated folate receptors: the effect of tumor size on targeting selectivity and radiochemical analysis of excreted tracer, in 12th International Symposium on Radiopharmaceutical Chemistry, Uppsala, Sweden.Google Scholar
  17. 17.
    Mathias, C. J., Wang, S., Waters, D. J., Turek, J. J., Low, P. S., and Green, M. A. (1997) Indium-111-DTPA-folate as a radiopharmaceutical for targeting tumor-associated folate binding protein (FBP), in Society of Nuclear Medicine 44th Annual Meeting, San Antonio, Texas.Google Scholar
  18. 18.
    Mathias, C. J., Wang, S., Lee, R. J., Waters, D. J., Low, P. S., and Green, M. A. (1996) Tumor-selective radiopharmaceutical targeting via receptor-mediated endocytosis of gallium-67-deferoxamine-folate. J. Nucleic Med. 37, 1003–1008.Google Scholar
  19. 19.
    Wang, S., Lee, R. J., Cauchon, G., Gorenstein, D. G., and Low, P. S. (1995) Delivery of antisense oligonucleotides against the human epidermal growth factor receptor into cultured KB cells with liposomes conjugated to folate via polyethyleneglycol. Proc. Natl. Acad. Sci. USA 92, 13,318–13,322.Google Scholar
  20. 20.
    Lee, R. J. and Huang, L. (1996) Folate-targeted, anionic liposome-entrapped polylysine-condensed DNA for tumor cell-specific gene transfer. J. Biol. Chem. 271, 8481–8487.CrossRefPubMedGoogle Scholar

Copyright information

© Humana Press Inc. 2000

Authors and Affiliations

  1. 1.Endocyte, Inc.West Lafayette
  2. 2.Department of ChemistryPurdue UniversityWest Lafayette

Personalised recommendations