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Drug Targeting pp 215-226 | Cite as

12 Making Fusion Toxins to Target Leukemia and Lymphoma

Protocol
Part of the Methods in Molecular Medicine™ book series (MIMM, volume 25)

Abstract

Recombinant toxins are cytotoxic proteins that are encoded by DNA sequences that can be expressed in prokaryotic or eukaryotic cells. The proteins contain both a ligand, for binding to cells, and a toxin, for killing the cells. In recombinant toxins the connection between the toxin and ligand is encoded by the DNA and not produced by chemical conjugation. Since recombinant toxins must bind specifically to cell surface molecules to kill cells, the number of possible recombinant toxin molecules that can bind to a cell is in the hundreds or thousands; hence, the toxins must be very potent.

Keywords

Inclusion Body Variable Domain Anion Exchange Resin Diphtheria Toxin Dialysis Buffer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc. 2000

Authors and Affiliations

  1. 1.Laboratory of Molecular Biology, Division of Cancer BiologyNational Cancer Institute, National Institutes of HealthBethesda

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