Abstract
During the development and maturation of hematopoetic precursors, certain enzymes and associated substances, such as glycogen, are produced in the developing cells (1,2), which, if detected, can provide important clues to the lineage and classification of leukemias. Although the evaluation of abnormal populations of white blood cells has been greatly refined by the use of immunohistochemistry, flow-cytometric analysis (3), and molecular studies, cytochemistry still plays an important role in the assessment of the abnormal marrow and peripheral blood smear. Cytochemical studies, therefore, still have a role to play in the evaluation of leukemias and other hematological diseases see Fig. 1 ).
Uses of cytochemical studies in the evaluation of blood and bone marrow smears.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Bainton D. F., Ullyot J. F., and Farquar, M. G. (1971) The development of neu-trophilic polymorphonuclear leukocytes in human bone marrow. J. Exp. Med. 134, 907–934.
Bainton, D. F. (1977) Differentiation of human neutrophilic granulocytes; normal and abnormal. Prog. Clin. Biol. Res. 13, 1–27.
Raimondi R., Pellizzari G., and Rodeghiero, F. (1993) Single step immunophenotyping of acute leukemias not classifiable by standard morphology and cytochemistry: a practical aproach. Haematologica 76, 66–67.
Bennett J.M., Catovsky D., Daniel M.T., Flandrin M.T., Galton D.A., Gralnick H.R., and Sultan, C. (1976) Proposals for the classification of the acute leukemias. French-American-British (FAB) co-operative group. Br. J. Haematology 33, 451–458.
Okun D. B. and Tanaka, K. R. (1978) Leukocyte alkaline phosphatase. Am. J. Hematol. 293–299.
Bennett J. M., Catovsky D., Daniel M. T., Flandrin G., Galton D. A., Gralnick H. R., and Sultan, C. (1982) Proposals for the classification of the myelodysplastic syndromes. Br. J. Haematolgy 51, 189–199.
Varela B. L., Chuang C., Woll J. E., and Bennett, J. M. (1985) Modifications in the classification of primary myelodysplastic syndromes: the addition of a scoring system. Hematol. Oncol. 3, 55–63.
Bennett J. M., Catovsky D., Daniel M. T., Flandrin G., Galton D. A., Gralnick H. R., and Sultan, C. (1989) Proposals for the classification of chronic B and T cell lympoid leukemias. French-American-British cooperative group. J. Clin. Pathol. 42, 567–568.
Murray, G. I. (1992) Enzyme histochemistry and immunohistochemistry with freeze dried or freeze substituted resin-embedded tissue. Histochem. J. 24, 399–408.
Breton-Gorius J. and Reyes, F. (1976) Ultrastructure of human bone marrow cell maturation. Int. Rev. Cytol. 46, 251–321.
Breton-Gorius J., Houssay D., and Dreyfus, B. (1975) Partial myeloperoxidase deficiency in a case of preleukemia, I. Studies of fine structure and peroxidase synthesis of promyelocytes. Br. J. Haematol. 30, 273–278.
Shibata A., Bennet J. M., Castoldi G. L., Catovisky D., Flandrin G., Jaffe E. S., Katayama I., Nanba K., Schmalzl F., and Yam, L. T. (1985) Recomended methods for cytological procedures in haematology. International Committee for Standardization in Haematology (ICSH). Clin. Lab. Hematol. 7, 55–74.
Nauseef, W. M. (1990) Myeloperoxidase deficiency. Hematol. Pathol. 4, 165–178.
Lehrer R. I., Goldberg L. S., Apple M. A., and Rosenthal, N. P. (1972) Refractory megaloblastic anemia with myeloperoxidase-deficient neutrophils. Ann. Intern. Med. 76, 447–453.
Hayhoe F. G. J. and Quaglino, D. (1980) in Hematological Cytochemistry, Churchill-Livingstone, Edinburgh.
Sheehan, H. L. (1939) The staining of leukocyte granules by Sudan Black B. Journal Path. Bact. 49, 580–585.
Sheehan H. L. and Storey, G. W. (1974) An improved method of staining leukocyte granules with Sudan Black B. Journal Path. Bact. 59, 336–341.
Martin Noya A., Garcia Luaces M., Picabea L., Bodineau C., Noguerol P., Parody R., and Rodrigues Fernandes, J. M. (1989) Sudan black B positive acute lymphoblastic leukemia. Sangre 34, 234–237.
Lilleyman J. S., Mills V., Sugden P. J., and Britton, J. A. (1979) Periodic acid_shiff reaction and prognosis in lymphoblastic leukemia. J. Clin. Pathol. 32, 158–161.
Daniel M. T., Flandrin G., Lejeune F., Liso P., and Lortholary, P. (1971) Specific Esterases of monocytes: their use in the classification of acute leukemias. Nouv. Rev. Fr. Hematol. 11, 233–239.
Li C. Y., Lam K. W., and Yam, L. T. (1973)Esterases in human leukocytes. J. Histochem. Cytochem. 21, 1–12.
Tolksdorf G. and Stein, H. (1979) Acid alpha-naphthyl acetate esterase in hairy cell leukemia cells and other cells of the hematopoietic system. Blut. 39, 165–176.
Tavassoli M. Shaklai M., and Crosby, W. H. (1979) Cytochemical diagnosis of acute myelomonocytic leukemia. Am. J. Clin. Pathol. 72, 59–62.
Crockard A., Chalmers D., Matutes E., and Catovsky, D. (1982) Cytochemistry of acid hydrolass in chronic B and T cell leukemias. Am. J. Clin. Pathol. 78, 437–444.
Okun D. B. and Tanaka, K. R. (1978) Leukocyte alkaline phosphatase. Am. J. Hematol. 4, 293–299.
Li C., Yam L. T., and Lau, K. W. (1970) Acid phosphatase isenzymes in human leukocytes in normal and pathogenic conditions. J. Histochem. Cytochem. 18, 473–481.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2001 Humana Press Inc.
About this protocol
Cite this protocol
Hanly, M.G. (2001). Methods in Leukocyte Cytochemistry. In: Faguet, G.B. (eds) Hematologic Malignancies. Methods in Molecular Medicine™, vol 55. Humana Press. https://doi.org/10.1385/1-59259-074-8:277
Download citation
DOI: https://doi.org/10.1385/1-59259-074-8:277
Publisher Name: Humana Press
Print ISBN: 978-0-89603-543-0
Online ISBN: 978-1-59259-074-2
eBook Packages: Springer Protocols