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FISH Analysis

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Hematologic Malignancies

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 55))

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Abstract

In situ hybridization of specific DNA or RNA sequences to cellular targets was developed over 20 yr ago (1,2). The early techniques employed isotopically labeled probes and subsequent autoradiographic detection using a photographic emulsion overlying the metaphase chromosomes, nuclei, or whole cells. However, autoradiography requires long exposure periods, and is not practical for clinical application. In the late 1970s, nonisotopic methods of nucleic acid labeling were developed. The subsequent improvements in the detection of reporter molecules using immunocytochemistry and immunofluorescence, in conjunction with advances in fluorescence microscopy and image analysis, have made the technique safer, faster and reliable.

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Author information

Authors and Affiliations

  1. DNA Diagnostics Department, St. Joseph’s Hospital and Medical Center, Phoenix, AZ

    Avery A. Sandberg

  2. Cytogenetics Laboratory, University of Utah Medical Center, Salt Lake City, UT

    Zhong Chen

Authors
  1. Avery A. Sandberg
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  2. Zhong Chen
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Editor information

Editors and Affiliations

  1. VA Medical Center and the Medical College of Georgia, Augusta, GA

    Guy B. Faguet

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© 2001 Humana Press Inc.

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Sandberg, A.A., Chen, Z. (2001). FISH Analysis. In: Faguet, G.B. (eds) Hematologic Malignancies. Methods in Molecular Medicine™, vol 55. Humana Press. https://doi.org/10.1385/1-59259-074-8:19

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  • DOI: https://doi.org/10.1385/1-59259-074-8:19

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-543-0

  • Online ISBN: 978-1-59259-074-2

  • eBook Packages: Springer Protocols

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