Abstract
Removal of arterial endothelium and damage to medial smooth muscle with a balloon embolectomy catheter lead to formation of a thin mural thrombus, platelet adhesion and degranulation, smooth muscle cell migration to the intima, and cell proliferation and matrix synthesis, ultimately producing a thickened neointimal layer. This model was developed initially by Baumgartner and Studer in the 1960s (1) and was modified (2) and used extensively throughout the 1970s and 1980s to develop our knowledge of vascular smooth muscle and endothelial cell kinetics following injury in adult animals (3). In the 1980s and 1990s it was used extensively to explore the effects of pharmacological agents that might influence vascular smooth muscle cell growth (4-7).
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References
Baumgartner, H. R. and Studer, A. (1966) Effects of vascular catheterization in normo-and hypercholesteremic rabbits. Pathol. Microbiol. 29, 393–405.
Clowes, A. W., Reidy, M. A., and Clowes, M. M. (1983) Mechanisms of stenosis after arterial injury. Lab. Invest. 49, 208–215.
Clowes, A.W., Clowes, M. M., and Reidy, M. A. (1986) Kinetics of cellular proliferation after arterial injury: III. Endothelial and smooth muscle growth in chronically denuded vessels. Lab. Invest. 54, 295–303.
Jackson, C. L. and Schwartz, S. M. (1992) Pharmacology of smooth muscle cell replication. Hypertension 20, 713–736.
Zempo, N., Koyama, N., Kenagy, R. D., Lea, H. J., and Clowes, A. W. (1996) Regulation of vascular smooth muscle cell migration and proliferation in vitro and in injured rat arteries by a synthetic matrix metalloproteinase inhibitor. Arterioscler. Thromb. Vasc. Biol. 16, 28–33.
Wong, J., Rauhoft, C., Dilley, R. J., Agratis, A., Jennings, G. L., and Bobik, A. (1997) Angiotensin-converting enzyme inhibition abolishes medial smooth muscle PDGF-AB biosynthesis and attenuates cell proliferation in injured carotid arteries: relationships to neointima formation. Circulation 96, 1631–1640.
Ward, M. R., Sasahara, T., Agrotis, A., Dilley, R. J., Jennings, G. L., and Bobik, A. (1998) Inhibitory effects of tranilast on expression of transforming growth factorbeta isoforms and receptors in injured arteries. Atherosclerosis 137, 267–275.
Campbell, J. H., Fennessy, P., and Campbell, G. R. (1992) Effect of perindopril on the development of atherosclerosis in the cholesterol-fed rabbit. Clin. Exp. Pharmacol. Physiol. Suppl. 19, 13–17.
Webster, W. S., Bishop, S. P., and Geer, J. C. (1974) Experimental aortic intimal thickening: II. Endothelialization and permeability. Am. J. Pathol. 76, 265–284.
Clowes, A. W., Collazzo, R. E., and Karnovsky, M. J. (1978) A morphologic and permeability study of luminal smooth muscle cells after arterial injury in the rat. Lab. Invest. 39, 141–150.
Lindner, V., Reidy, M. A., and Fingerle, J. (1989) Regrowth of arterial endothelium. Denudation with minimal trauma leads to complete endothelial cell regrowth. Lab. Invest. 61, 556–563.
Reidy, M. A. and Schwartz, S. M. (1981) Endothelial regeneration: III. Time course of intimal changes after small defined injury to rat aortic endothelium. Lab. Invest. 44, 301–308.
Koyama, H. and Reidy, M. A. (1997) Reinjury of arterial lesions induces intimal smooth muscle cell replication that is not controlled by fibroblast growth factor 2. Circ. Res. 80, 408–417.
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© 2001 Humana Press Inc., Totowa, NJ
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Dilley, R.J. (2001). Mechanical Injury Models. In: Drew, A.F. (eds) Atherosclerosis. Methods in Molecular Medicine™, vol 52. Humana Press. https://doi.org/10.1385/1-59259-073-X:7
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DOI: https://doi.org/10.1385/1-59259-073-X:7
Publisher Name: Humana Press
Print ISBN: 978-0-89603-751-9
Online ISBN: 978-1-59259-073-5
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