Isolation and Characterization of Glycosaminoglycans from Human Atheromatous Vessels

  • Eleni Papakonstantinou
  • Michael Roth
  • George Karakiulakis
Protocol
Part of the Methods in Molecular Medicine™ book series (MIMM, volume 52)

Abstract

The complex cascade of events leading to the formation of atheromatous plaques depends on the interaction between several cell types, growth factors, cytokines, and molecules of the extracellular matrix (ECM) (1). Among different molecules of the ECM involved in atherogenesis, the glycosaminoglycan(GAGs) have been reported to contribute to key events leading to the formation of atherosclerotic lesions (2). GAGs are linear acidic polysaccharides of variable length and composition, which occur either in free form or attached to a protein core to form proteoglycans (3). On the basis of their composition, GAGs are grouped into four major categories: hyaluronic acid, heparin and heparan sulfate, chondroitin and dermatan sulfates, and keratan sulfate. ECM GAGs provide structural links between fibrous and cellular elements, contribute to viscoelastic properties, regulate permeability and retention of plasma components within the matrix (2,4), inhibit vascular cell growth (5), affect hemostasis and platelet aggregation (6), and interact with lipoproteins (7) and various growth factors (8,9).

Keywords

HPLC Dust Hexane Heparin Sedimentation 

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Copyright information

© Humana Press Inc., Totowa, NJ 2001

Authors and Affiliations

  • Eleni Papakonstantinou
    • 1
  • Michael Roth
    • 2
  • George Karakiulakis
    • 1
  1. 1.Department of Pharmacology, School of MedicineAristotle University of ThessalonikiGreece
  2. 2.Institute of Respiratory Medicine, Royal Prince Alfred HospitalUniversity of SydneyCamperdownAustralia

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