9 Assay for Serum Glycated Lipoproteins
Modified low density lipoprotein (LDL) is considered to be a risk factor for the development and progression of atherosclerosis (1-3). Glycated LDL and oxidized LDL are the only modified lipoproteins that exist naturally in the human body, but oxidized LDL does not exist in the circulation because of the presence of many antioxidizing agents (4). LDL cannot be oxidized until it has entered the arterial wall from the circulation. The only modified LDL that exists in the circulation is glycated LDL. It has been shown to be more easily oxidized than native LDL (5-7). These findings indicate that the glycation of LDL may be the initial step in the development of atherosclerosis.
KeywordsArterial Wall System Controller Analytical Temperature Column Oven Unbind Fraction
- 3.Makita, T., Tanaka, A., and Numano, F.(1999) Effect of glycated low density lipoprotein on smooth muscle cell proliferation. Int. Angiol., in press.Google Scholar
- 4.Colaco, C. A. L. S. and Roser, B. J.(1994) Atherosclerosis and glycation. Bio As says 16, 145–147.Google Scholar
- 5.Lyon, T. J. (1992) Lipoprotein glycation and its metabolic consequences. Diabetes 41, 67–73.Google Scholar
- 12.Dhima, K., Ito, N., Abe, F., Hirota, M., Yano, M., Yamamoto, Y., Uchida, T., and Noguchi, K. (1988) High performance liquid chromatography assay of serum glycated albumin. Diabetology 31, 327–631.Google Scholar
- 14.Okazaki, M., Sasamoto, K., Muramatsu, T., and Hosaki, S. (1997) Analysis of plasma lipoproteins by gel permeation chromatography, in Handbook of Lipoprotein Testing. (Rifai, N., ed.), AACC press, Washington, DC, pp. 531–548.Google Scholar