Abstract
The mitogen-activated protein kinases (MAPK) are an expanding family of proline-directed serine/threonine kinases that are activated, following their dual phosphorylation at conserved threonine and tyrosine residues, by a family of MAPK kinases (MEK). Presently, the MAPK family can be divided into three groups: the extracellular signal-regulated kinases (ERK), composed of ERK-1/2/3/4/5, the c-jun N-terminal kinases (JNK)-46/54; and the p38 MAP kinase (p38 MAPK) composed of p38/p38β/p38γ/p38δ (1). Of these enzymes, the authors have detected ERK-1/2/3/5, JNK-46/54, and p38 in guinea pig peritoneal eosinophils by Western analysis: ERK-4 and p38β were apparently absent.
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References
Widmann, C., Gibson, S., Jarpe, M. B., and Johnson, G. L. (1999) Mitogenactivated protein kinase: conservation of a three-kinase module from yeast to human. Physiol. Rev. 79, 143–180.
Alam, R., Pazdrak, K., Stafford, S., and Forsythe, P. (1995) The interleukin-5/ receptor interaction activates Lyn and Jak2 tyrosine kinases and propagates signals via the Ras-Raf-1-MAP kinase and the Jak-STAT pathways in eosinophils. Int. Arch. Allergy Immunol. 107, 226–227.
Pazdrak, K., Schreiber, D., Forsythe,., Justement, L., and Alam, R. (1995) The intracellular signal transduction mechanism of interleukin 5 in eosinophils: the involvement of lyn tyrosine kinase and the Ras-Raf-1-MEK-microtubuleassociated protein kinase pathway. J. Exp. Med. 181, 1827–1834.
Bates, M. E., Bertics, P. J., and Busse, W. W. (1996) IL-5 activates a 45-kilodalton mitogen-activated protein (MAP) kinase and Jak-2 tyrosine kinase in human eosinophils. J. Immunol. 156, 711–718.
Hiraguri, M., Miike, S., Sano, H., Kurasawa, K., Saito, Y., and Iwamoto, I. (1997) Granulocyte-macrophage colony-stimulating factor and IL-5 activate mitogenactivated protein kinase through Jak2 kinase and phosphatidylinositol 3-kinase in human eosinophils. J. Allergy Clin. Immunol. 100, S45–51.
Coffer, P. J., Schweizer, R. C., Dubois, G. R., Maikoe, T., Lammers, J. W., and Koenderman, L. (1998) Analysis of signal transduction pathways in human eosinophils activated by chemoattractants and the T-helper 2-derived cytokines interleukin-4 and interleukin-5. Blood 91, 2547–2557.
Pazdrak, K., Adachi, T., and Alam, R. (1997) Src homology 2 protein tyrosine phosphatase (SHPTP2)/Src homology 2 phosphatase 2 (SHP2) tyrosine phosphatase is a positive regulator of the interleukin 5 receptor signal transduction pathways leading to the prolongation of eosinophil survival. J. Exp. Med. 186, 561–568.
Lindsay, M. A., Hadded, E.-L., Rousell, J., Teixiera, M. M., Hellewell, P. G., Barnes, P. J., and Giembycz, M. A. (1998) Role of mitogen-activated protein kinases and tyrosine kinases during leukotriene B4 induced eosinophil activation. J. Leuc. Biol. 64, 555–562.
Kankannranta, H., de Souza, P. M., Salmon, M., Barnes, P. J., Giembycz, M. A., and Lindsay, M. A. (1999) SB203580, an inhibitor of p38 mitogen-activated protein kinase, enhances constitutive apoptosis of cytokine deprived human eosinophils. J. Pharmacol. Exp. Ther. 290, 621–628.
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de Souza, P.M., Kankaanranta, H., Giembycz, M.A., Lindsay, M.A. (2000). MAP Kinase Expression in Eosinophils. In: Fan Chung, K., Adcock, I. (eds) Asthma. Methods in Molecular Medicine™, vol 44. Humana Press. https://doi.org/10.1385/1-59259-072-1:91
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DOI: https://doi.org/10.1385/1-59259-072-1:91
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