Abstract
Stimulation by catecholamine agonists of the β-adrenergic coupled adenylyl cyclase (βAR-AC) system, expressed on human tracheobronchial epithelial cells (ECs), elicits a variety of cellular responses that favorably affect airway function, the intensity of the inflammatory reaction, and even the integrity of the epithelial lining (1–6). For example, β-agonist-stimulated production of second messenger, cyclic adenosine monophosphate (cAMP), enhances salt and water exchange (2), ciliary beating (3), mucus secretion by goblet cells (1,4), proliferation of airway ECs (5), and protection against free radical induced injury (6).
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Nijkamp, F. P., Engels, F., Henricks, P. A. J., and Vanoosterhout, A. J. M. (1992) Mechanisms of β-adrenergic receptor regulation in lungs and its implications for physiological responses. Physiol. Rev. 72, 323–366.
Smith, P. L., Welsh, M. J., Stoff, S. J., and Frizzell, R. A. (1982) Chloride secretion by canine tracheal epithelium: role of intracellular cAMP levels. J. Memb. Biol. 70, 215–226.
Sanderson, M. J. and Dirksen, E. R. (1989) Mechanosensitive and β-adrenergic control of the ciliary beat frequency of mammalian respiratory tract cells in culture. Am. Rev. Respir. Dis. 139, 432–440.
Barnes, P. J., Chung, K. F., and Page, C. P. (1988) Inflammatory mediators and asthma. Pharmacol. Rev. 40, 49–84.
Wiley, J., Laveck, M. A., McClendon, I. A., and Lechner, J. F. (1985) Relationship of ornithine decarboxylase activity and cAMP metabolism to proliferation of normal human bronchial epithelial cells. J. Cell Physiol. 124, 207–212.
Koyama, S., Rennard, S. I., Claasen, L., and Robins, R. A. (1991) Dibutryly cAMP, prostaglandin E2, and antioxidants protect cultured bovine bronchial epithelial cells from endotoxin. Am. J. Physiol. 261, L126–L132.
Davis, P. B., Silski, C. L., Kercsmar, C. M., and Infeld, M. D. (1990) Beta adren-ergic receptors on human tracheal epithelial cells in primary culture. Am. J. Physiol. 258, C71–C76.
Kercsmar, C. M., Infeld, M. D., Silski, C. L., and Davis, P. B. (1990) Adenosine 3:5 monophosphate synthesis by human tracheal epithelial cells. Am. J. Respir. Cell Mol. Biol. 2, 33–39.
Penn, R., Kelsen, S. G., and Benovic, J. L. (1994) Regulation of β-agonist and prostaglandin E2-mediated adenylyl cyclase activity in human airway epithelial cells. Am. J. Respir. Cell Mol. Biol. 11, 496–505.
Kelsen, S. G., Mardini, I. A., Zhou, S., Benovic, J. L., and Higgins, N. C. (1992) Technique to harvest viable tracheobronchial epithelial cells form living human donors. Am. J. Respir. Cell Mol. Biol. 7, 66–72.
Kelsen, S. G., Higgins, N. C., Zhou, S., Mardini, I. A., and Benovic, J. L. (1995) Expression and function of the beta-adrenergic receptor-coupled adenylyl cyclase system on human airway epithelial cells. Am. J. Respir. Crit. Care Med. 152, 1774–1783.
Smith, D. L. and Deshazo, R. D. (1993) Bronchoalveolar lavage in asthma. An update and perspective. Am. Rev. Respir. Dis. 148, 523–532.
O’Guin, W. M., Schermer, S., and Sun, T. (1990) Immunofloresecnce staining of keratin filaments in cultured epithelial cells. J. Tissue Culture Methods 9, 123–128.
Scatchard, G. (1949) Attraction of proteins for small molecules and ions. Ann. NY Acad. Sci. 51, 660–672.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2000 Humana Press Inc.
About this protocol
Cite this protocol
Kelsen, S.G., Ciccolella, D., Brennan, K. (2000). Brush Biopsy and Culture of Airway Epithelial Cells: β-Adrenergic Receptor System Function . In: Fan Chung, K., Adcock, I. (eds) Asthma. Methods in Molecular Medicine™, vol 44. Humana Press. https://doi.org/10.1385/1-59259-072-1:41
Download citation
DOI: https://doi.org/10.1385/1-59259-072-1:41
Publisher Name: Humana Press
Print ISBN: 978-0-89603-626-0
Online ISBN: 978-1-59259-072-8
eBook Packages: Springer Protocols