Abstract
In 1981, a novel transforming gene called neu, related to, but distinct from, the c-erbB protooncogene, was identified (1-3). In 1985, two groups independently isolated identical erbB-related genes from human DNA that they called HER-2 (4) and c-erbB-2 (5) located at chromosome band 17q11.2 and encoding a 185,000 Dalton tyrosine kinase (2,4). Studies conducted on human breast cancers showed 28% to have amplified HER2/neu present (7) and it was suggested that amplification or increased protein expression might confer a selective advantage (6). Over the last ten years, many studies have confirmed the value of using overexpression or amplification of the HER2/neu gene as a predictor of poor outcome in cases of breast cancer (7-9). Clinical trials are currently underway using anti-HER2/neu in an attempt to destroy malignant breast cells (10,11).
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Shih, C., Padhy, L. C., Murray, M., and Weinberg, R. A. (1981) Transforming genes of carcinomas and neuroblasts introduced into mouse fibroblasts. Nature 290, 261–264.
Schechter, A. L., Stern, D. F., Vaidyanathan, L., Decker, S. J., Drebin, J. A., Greene, M. I., and Weinberg, R. A. (1984) The neu oncogene: an erb-B-related gene encoding a 185,000-Mr tumor antigen. Nature 312, 513–516.
Semba, K., Kamata, N., Toyoshima, K., and Yamamoto, T. (1985) A v-erbB-related protooncogene, c-erbB-2, is distinct from the c-erbB-1/epidermal growth factor-receptor gene and is amplified in a human salivary gland adenocarcinoma. Proc. Natl. Acad. Sci. USA 82, 6497–6501.
Coussens, L., Yang-Feng, T. L., Liao, Y-C., Chen, E., Gray, A., McGrath, J., et al. (1985) Tyrosine kinase receptor with extensive homology to EGF receptor shares chromosomal location with neu oncogene. Science 230, 1132–1139.
Schechter, A. L., Hung, M-C., Vaidyanathan, L., Weinberg, R. A., Yang-Feng, T. L., Francke, U., et al. (1985) The neu gene: an erbB-homologous gene distinct from and unlinked to the gene encoding the EGF receptor. Science 229, 976–978.
King, C. R., Kraus, M. H., and Aaronson, S. A. (1985) Amplification of a novel v-erbB-related gene in a human mammary carcinoma. Science 229, 974–976.
Slamon, D. J., Clark, G. M., Wong, S. G., Levin, W. J., Ullrich, A., and McGuire, W. L. (1987) Human breast cancer: correlation of relapse and survival with amplification of the HER-2/neu oncogene. Science 235, 177–182.
Paterson, M. C., Dietrich, K. D., Danyluk, J., Paterson, A. H. G., Lees, A. W., Jamil, N., et al. (1991) Correlation between c-erbB-2 amplification and risk of recurrent disease in node-negative breast cancer. Cancer Res. 51, 556–567.
Menard, S., Casalini, P., Pilotti, S., Cascinelli, N., Rilke, F., and Colnaghi, M. I. (1996) No additive impact on patient survival of the double alteration of p53 and c-erbB-2 in breast carcinomas. JNCI 88, 1002,1003.
Weiner, L. M., Clark, J. I., Davey, M., Li, W. S., Garcia de Palazzo, I., Ring, D. B., and Alpaugh, R. K. (1995) Phase I trial of 2B1, a bispecific monoclonal antibody targeting c-erbB-2 and Fc gamma RIII. Cancer Res. 55, 4586–4593.
Baselga, J., Tripathy, D., Mendelsohn, J., Baughman, S., Benz, C. C., Dantis, L., et al. (1996) Phase II study of weekly intravenous recombinant humanized anti-p185HER2 monoclonal antibody in patients with HER2/neu-overexpressing metastatic breast cancer. J. Clin. Oncol. 14, 737–744.
Press, M. F., Hung, G., Godolphin, W., and Slamon, D. J. (1994) Sensitivity of HER-2/neu antibodies in archival tissue samples: potential source of error in immunohistochemical studies of oncogene expression. Cancer Res. 54, 2771–2777.
Busmanis, I., Feleppa, F., Jones, A., McGrath, K. M., Reed, R., Collins, J., et al. (1994) Analysis of cerbB2 expression using a panel of 6 commercially available antibodies. Pathology 26, 261–267.
Sauter, G., Moch, H., Moore, D., Carroll, P., Chew, K., Mihatsch, M. J., et al. (1993) Heterogeneity of erbB-2 gene amplification in bladder cancer. Cancer Res. 54, 2199–2203.
Kallioniemi, O.-P., Kallioniemi, A., Kurisu, W., Thor, A., Chen, L.-C., Smith, H. S., et al. (1992) ERBB2 amplification in breast cancer analyzed by fluorescence in situ hybridization. Proc. Natl. Acad. Sci. USA 89, 130–134.
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Young, S.R., Liu, WH., Wang, ZR. (2000). Gene Amplification of c-erbB-2 Detected by FISH. In: Bartlett, J.M.S. (eds) Ovarian Cancer. Methods in Molecular Medicine™, vol 39. Humana Press. https://doi.org/10.1385/1-59259-071-3:237
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DOI: https://doi.org/10.1385/1-59259-071-3:237
Publisher Name: Humana Press
Print ISBN: 978-0-89603-583-6
Online ISBN: 978-1-59259-071-1
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