Skip to main content

Interphase Cytogenetics in Paraffin-Embedded Ovarian Tissue

  • Protocol
Ovarian Cancer

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 39))

  • 932 Accesses

Abstract

Interphase cytogenetics using formalin-fixed/paraffin-embedded tissue is now a well-established technique, which renders it possible to obtain “cytogenetic information” from interphase nuclei of solid tumors (1,2, for ovarian cancer, e.g., 3-8). It is the only tool to investigate specific numerical chromosomal aberrations (3-7), amplification

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Wolfe, K. Q. and Herrington, C. S. (1997) Interphase cytogenetics and pathology: a tool for diagno-sis and research. J. Pathol. 181, 359–361.

    Article  CAS  PubMed  Google Scholar 

  2. Hackel, C. and VarellaGarcia, M. (1997) Interphase cytogenetics using fluorescence in situ hybridization: an overview of its application to diffuse and solid tissue. Braz. J. Genet. 20, 97–106.

    Article  Google Scholar 

  3. Wolf, G. N., Abdul-Karim, F. W., Schork, N. J., and Schwartz, S. (1996) Origins of heterogeneous ovarian carcinomas. A molecular cytogenetic analysis of histologically benign, low malignant potential, and fully malignant components. Am. J. Pathol. 149, 511–520.

    CAS  PubMed  Google Scholar 

  4. Persons, D. L., Hartmann, L. C., Herath, J. F., Borell, T. J., Keeney, G. L., and Jenkins, R. B. (1993) Interphase molecular cytogenetic analysis of epithelial ovarian tumors. Am. J. Clin. Pathol. 142, 733–741.

    CAS  Google Scholar 

  5. Liehr, T., Atanasov, N., Tulusan, H. A., and Gebhart, E. (1993) Amplification of proto-oncogenes in human ovarian carcinomas. Int. J. Oncol. 2, 155–160.

    Google Scholar 

  6. Neubauer, S., Liehr, T., Tulusan, H. A., and Gebhart, E. (1994) Interphase cytogenetics by FISH on archival paraffin material and cultured cells of human ovarian tumors. Int. J. Oncol. 4, 317–321.

    Google Scholar 

  7. Liehr, T., Stübinger, A., Thoma, K., Tulusan, H. A., and Gebhart, E. (1994) Comparative interphase cytogenetics using FISH on human ovarian carcinomas. Anticancer Res. 14, 183–188.

    CAS  PubMed  Google Scholar 

  8. Young, S. R., Liu, W.-H., Brock, J.-A., and Smith, S. T. (1996) ERBB2 and chromosome 17 centromere studies of ovarian cancer by fluorescence in situ hybridization. Genes Chr. Cancer 16, 130–137.

    Article  CAS  Google Scholar 

  9. Tsuji, T., Tagawa, Y., Hisamatsu, T., Nakamura, S., Terada, R., Sawai, T., et al. (1997) p53 alter-ations and chromosome 17 aberrations in non-small cell lung cancer. Gan To Kagaku Ryoho 24, 263–268.

    PubMed  Google Scholar 

  10. Yoshida, H., Nagao, K., Ito, H., Yamamoto, K., and Ushigome, S. (1997) Chromosomal translocations in human soft tissue sarcomas by interphase fluorescence in situ hybridization. Pathol. Internat. 47, 222–229.

    Article  CAS  Google Scholar 

  11. Mesker, W. E., Alers, J. C., Sloos, W. C. R., Vrolijk, H., Raap, A. K., Dekken, H. V., and Tanke, H. J. (1996) Automated assessment of numerical chromosomal aberrations in paraffin embedded prostate tumor cells stained by in situ hybridization. Cytometry 26, 298–304.

    Article  CAS  PubMed  Google Scholar 

  12. Köpf, I., Hanson, C., Delle, U., Verbiené, I., and Weimarck, A. (1996) A rapid and simplified technique for analysis of archival formalin-fixed, paraffin-embedded tissue by fluorescence in situ hybridization (FISH). Anticancer Res. 16, 2533–2536.

    PubMed  Google Scholar 

  13. Qian, J. Q., Bostwick, D. G., Takahashi, S., Borell, T. J., Brown, J. A., Lieber, M. M., and Jenkins, R. B. (1996) Comparison of fluorescence in situ hybridization analysis of isolated nuclei and routine histological sections from paraffin-embedded prostatic adenocarcinoma specimens. Am. J. Pathol. 149, 1193–1199.

    CAS  PubMed  Google Scholar 

  14. Hopman, A. H. N., Van Hooren, E., Van de Kaa, C. A., Vooijs, P. G. P., and Ramaekers, F. C. S. (1991) Detection of numerical chromosome aberrations using in situ hybridization in paraffin sections of routinely processed bladder cancers. Mod. Pathol. 4, 503–513.

    CAS  PubMed  Google Scholar 

  15. Dhingra, K., Sahin, A., Supak, J., Kim, S. Y., Hortobagyi, G., and Hittelman, W. N. (1992) Chromosome in situ hybridization on formalin-fixed mammary tissue using non-isotopic, nonfluorescent probes: technical considerations and biological implications. Breast Cancer Res. Treat. 23, 201–210.

    Article  CAS  PubMed  Google Scholar 

  16. Liehr, T., Grehl, H., and Rautenstrauss, B. (1995) FISH analysis of interphase nuclei extracted from paraffin-embedded tissue. Trends Genet. 11, 377,378.

    Google Scholar 

  17. Liehr, T., Thoma, K., Kammler, K., Gehring, C., Ekici, A., Bathke, K. D., et al. (1995) Direct preparation of uncultured EDTA-treated or heparinized blood for interphase FISH analysis. Appl. Cytogenet. 21, 185–188.

    Google Scholar 

  18. Fox, J. L., Hsu, P. H., Legator, M. S., Morrison, L. E., and Seelig, S. A. (1995) Fluorescence in situ hybridization: powerful molecular tool for cancer prognosis. Clin. Chem. 41, 1554–1559.

    CAS  PubMed  Google Scholar 

  19. Mark, H. F., Jenkins, R., and Miller, W. A. (1997) Current applications of molecular cytogenetic technologies. Ann. Clin. Lab. Sci. 27, 47–56.

    CAS  PubMed  Google Scholar 

  20. Wolff, E., Liehr, T., Vorderwülbecke, U., Tulusan, A. H., Husslein, E. M., and Gebhart, E. (1997) Frequent gains and losses of specific chromosome segments in human ovarian carcinomas shown by comparative genomic hybridization. Int. J. Oncol. 11, 19–23.

    CAS  Google Scholar 

  21. Neubauer, S., Gebhart, E., Schmitt, G., Birkenhake, S., and Dunst, J. (1996) Is chromosome in situ suppression (CISS) hybridization suited as a predictive test for intrinsic radiosensitivity in cancer patients? Int. J. Oncol. 8, 707–712.

    Google Scholar 

  22. Kraus, C., Liehr, T., Hülsken, J., Behrens, J., Birchmeier, W., Greschick, K. H., and Ballhausen, W. G. (1994) Localization of the human β-catenin gene (CTNNB1) to 3p21: a region implicated in tumor development. Genomics 23, 272–274.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2000 Humana Press Inc.

About this protocol

Cite this protocol

Neubauer, S., Liehr, T. (2000). Interphase Cytogenetics in Paraffin-Embedded Ovarian Tissue. In: Bartlett, J.M.S. (eds) Ovarian Cancer. Methods in Molecular Medicine™, vol 39. Humana Press. https://doi.org/10.1385/1-59259-071-3:223

Download citation

  • DOI: https://doi.org/10.1385/1-59259-071-3:223

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-583-6

  • Online ISBN: 978-1-59259-071-1

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics