Abstract
Resulting from problems of low yield of metaphases and poor-quality chromosomes, detection of cytogenetic changes in solid tumors has been relatively limited (1). The advent of fluorescence in situ hybridization (FISH) rendered it possible to obtain “cytogenetic information” from interphase nuclei (2,3). Since that time, interphase cytogenetics has become a powerful tool to investigate numerical chromosomal aberrations (4,5), amplification of oncogenes (6), or deletion of tumor suppressor genes in solid tumors (7), including ovarian cancer. Both fresh tumor material (8-10) or formalin-fixed and, subsequently, paraffin-embedded tissue have been used for interphase cytogenetic studies in ovarian tumors (4-6 8-10).
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Heim, S. and Mitelman, F. (1995) Cancer Cytogenetics. 2nd Ed., Wiley-Liss, New York.
Langer, P. R., Waldrop, A. A., and Ward, D. C. (1981) Enzymatic synthesis of biotin-labeled polynucleitides: novel nucleic acid affinity probes. Proc. Natl. Acad. Sci. USA 78, 6633–6637.
Pinkel, D., Straume, T., and Gray, J. W. (1986) Cytogenetic analysis using quantitative, high sensitivity, fluorescence hybridization. Proc. Natl. Acad. Sci. USA 83, 2934–2938.
Persons, D. L., Hartmann, L. C., Herath, J. F., Borell, T. J., Keeney, G. L., and Jenkins, R. B. (1993) Interphase molecular cytogenetic analysis of epithelial ovarian tumors. Am. J. Clin. Pathol. 142, 733–741.
Gibas, Z. and Talermann, A. (1993) Analysis of chromosome aneuploidy in ovarian dysgerminoma by flow cytometry and fluorescence in situ hybridization. Diagn. Mol. Pathol. 2, 50–56.
Young, S. R., Liu, W.-H., Brock, J.-A., and Smith, S. T. (1996) ERBB2 and chromosome 17 centromere studies of ovarian cancer by fluorescence in situ hybridization. Genes Chr. Cancer 16, 130–137.
Tsuji, T., Tagawa, Y., Hisamatsu, T., Nakamura, S., Terada, R., Sawai, T., et al. (1997) p53 alterations and chromosome 17 aberrations in non-small cell lung cancer. Gan To Kagaku Ryoho 24Suppl. 2, 263–268.
Liehr, T., Atanasov, N., Tulusan, H. A., and Gebhart, E. (1993) Amplification of proto-oncogenes in human ovarian carcinomas. Int. J. Oncol. 2, 155–160.
Neubauer, S., Liehr, T., Tulusan, H. A., and Gebhart, E. (1994) Interphase cytogenetics by FISH on archival paraffin material and cultured cells of human ovarian tumors. Int. J. Oncol. 4, 317–321.
Liehr, T., Stübinger, A., Thoma, K., Tulusan, H. A., and Gebhart, E. (1994) Comparative interphase cytogenetics using FISH on human ovarian carcinomas. Anticancer Res. 14, 183–188.
Long, A. A., Komminoth, P., Lee, E., and Wolfe, H. J. (1993) Comparison of indirect and direct in-situ polymerase chain reaction in cell preparations and tissue sections. Detection of viral DNA, gene rearrangements and chromosomal translocations. Histochem. 99, 151–162.
Dhingra, K., Sahin, A., Supak, J., Kim, S. Y., Hortobagyi, G., and Hittelman, W. N. (1992) Chromosome in situ hybridization on formalin-fixed mammary tissue using non-isotopic, nonfluorescent probes: technical considerations and biological implications. Breast Cancer Res. Treat. 23, 201–210.
Hedley, D. W., Friedlander, M. L., Taylor, I. W., Rugg, C. A., and Musgrove, E. A. (1983) Method for analysis of cellular DNA content of paraffin-embedded pathological material using flow cytometry. J. Histochem. Cytochem. 31, 1333–1335.
Liehr, T., Grehl, H., and Rautenstrauss, B. (1996) A rapid method for FISH analysis on interphase nuclei extracted from cryofixed tissue. Trends Genet. 12, 505,506.
Liehr, T., Thoma, K., Kammler, K., Gehring, C., Ekici, A., Bathke, K. D., et al. (1995) Direct preparation of uncultured EDTA-treated or heparinized blood for interphase FISH analysis. Appl. Cytogenet. 21, 185–188.
Fox, J. L., Hsu, P. H., Legator, M. S., Morrison, L. E., and Seelig, S. A. (1995) Flourescence in situ hybridization: powerful molecular tool for cancer prognosis. Clin. Chem. 41, 1554–1559.
Mark, H. F., Jenkins, R., and Miller, W. A. (1997) Current applications of molecular cytogenetic technologies. Ann. Clin. Lab. Sci. 27, 47–56.
Wolff, E., Liehr, T., Vorderwülbecke, U., Tulusan, A. H., Husslein, E. M., and Gebhart, E. (1997) Frequent gains and losses of specific chromosome segments in human ovarian carcinomas shown by comparative genomic hybridization. Int. J. Oncol. 11, 19–23.
Neubauer, S., Gebhart, E., Schmitt, G., Birkenhake, S., and Dunst, J. (1996) Is chromosome in situ suppression (CISS) hybridization suited as a predictive test for intrinsic radiosensitivity in cancer patients? Int. J. Oncol. 8, 707–712.
Kraus, C., Liehr, T., Hülsken, J., Behrens, J., Birchmeier, W., Greschick, K. H., and Ballhausen, W. G. (1994) Localization of the human β-catenin gene (CTNNB1) to 3p21: a region implicated in tumor development. Genomics 23, 272–274.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2000 Humana Press Inc.
About this protocol
Cite this protocol
Liehr, T. (2000). Interphase Cytogenetics in Frozen Ovarian Tumor Tissue. In: Bartlett, J.M.S. (eds) Ovarian Cancer. Methods in Molecular Medicine™, vol 39. Humana Press. https://doi.org/10.1385/1-59259-071-3:215
Download citation
DOI: https://doi.org/10.1385/1-59259-071-3:215
Publisher Name: Humana Press
Print ISBN: 978-0-89603-583-6
Online ISBN: 978-1-59259-071-1
eBook Packages: Springer Protocols