Skip to main content
SpringerLink
Log in

Cytogenetics of Ovarian Cancer Technical Overview

  • Protocol
Ovarian Cancer

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 39))

  • 931 Accesses

Abstract

Cytogenetics in ovarian cancer has been restricted to conventional cytogenetic analysis of G-banded metaphase chromosomes up to the early 1980s. The detection of cytogenetic changes in solid tumors was relatively limited, as cytogenetic preparations from solid tumor tissue often have a low yield of metaphases with poor quality chromosomes (1). Following the advent of the fluorescence in situ hybridization (FISH) technique (2,3) it became possible to obtain huge amounts of additional cytogenetic information from the material available. This fact is demonstrated very clearly by the following comparison: Mitelman summarizes in the 4th edition of Catalog of Chromosome Aberrations in Cancer only 185 cases of ovarian cancer, which were characterized by conventional cytogenetic analysis between about 1970 to 1991 (4). On the other hand, 135 cases of ovarian cancer have been analyzed by means of comparative genomic hybridization (CGH) in the shorter time period 1995 to 1997 (5-8).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Heim, S. and Mitelman, F. (1995) Cancer Cytogenetics. 2nd Ed., Wiley-Liss, New York.

    Google Scholar 

  2. Langer, P. R., Waldrop, A. A., and Ward, D. C. (1981) Enzymatic synthesis of biotin-labeled poly-nucleotides: Novel nucleic acid affinity probes. Proc. Natl. Acad. Sci. USA 78, 6633–6637.

    Article  CAS  PubMed  Google Scholar 

  3. Pinkel, D., Straume, T., and Gray, J. W. (1986) Cytogenetic analysis using quantitative, high sensitivity, fluorescence hybridization. Proc. Natl. Acad. Sci. USA 83, 2934–2938.

    Article  CAS  PubMed  Google Scholar 

  4. Mitelman, F. (1991) Catalog of Chromosome Aberrations in Cancer. 4th Ed., Wiley-Liss, New York.

    Google Scholar 

  5. Iwabuchi, H., Sakamoto, M., Sakanaga, H., Ma, Y. Y., Carcangiu, M. L., Pinkel, D., et al. (1995) Genetic analysis of benign, low-grade, and high-grade ovarian tumors. Cancer Res. 55, 6172–6180.

    CAS  PubMed  Google Scholar 

  6. Arnold, N., Hagele, L., Walz, L., Schempp, W., Pfisterer, J., Bauknecht, T., and Kiechle, M. (1996) Overrepresentation of 3q and 8q material and loss of 18q material are recurrent findings in advanced human ovarian cancer. Genes Chr. Cancer 16, 46–54.

    Article  CAS  Google Scholar 

  7. Wasenius, V. M., Jekunen, A., Monni, O., Joensuu, H., Aebi, S., Howell, S. B., and Knuutila, S. (1997) Comparative genomic hybridization analysis of chromosomal changes occurring during development of acquired resistance to cisplatin in human ovarian carcinoma cells. Genes Chr. Cancer 18, 286–291.

    Article  CAS  Google Scholar 

  8. Wolff, E., Liehr, T., Vorderwülbecke, U., Tulusan, A. H., Husslein, E. M., and Gebhart, E. (1997) Frequent gains and losses of specific chromosome segments in human ovarian carcinomas shown by comparative genomic hybridization. Int. J. Oncol. 11, 19–23.

    CAS  Google Scholar 

  9. Alberts, B., Bray, D., Lewis, J., Raff, M., Roberts, K., and Watson, J. D. (1994) Molecular Biology of the Cell. 3rd Ed., Garland, New Garland, New York, London.

    Google Scholar 

  10. Liehr, T., Stübinger, A., Thoma, K., Tulusan, H. A., and Gebhart, E. (1994) Comparative interphase cytogenetics using FISH on human ovarian carcinomas. Anticancer Res. 14, 183–188.

    CAS  PubMed  Google Scholar 

  11. Augustus, M., Brüderlein, S., and Gebhart, E. (1986) Cytogenetics and cell cycle studies in meta-static cells from ovarian carcinomas. Anticancer Res. 6, 283–290.

    CAS  PubMed  Google Scholar 

  12. Di Cicco, R. A. and Piver, M. S. (1992) The genetics of ovarian cancer. Cancer Invest. 10, 135–141.

    Article  Google Scholar 

  13. Górski, G. K., McMorrow, L. E., Blumstein, L., Faasse, D., and Donaldson, M. H. (1992) Trisomy 14 in two cases of granulosa cell tumor of the ovary. Cancer Genet. Cytogenet. 60, 202–205.

    Article  PubMed  Google Scholar 

  14. Speleman, F., Dermaut, B., De Potter, C. R., Van Gele, M., Van Roy, N., De Paepe, A., and Laureys, G. (1997) Monosomy 22 in a mixed germ cell-sex cord-stromal tumor of the ovary. Genes Chr. Cancer 19, 192–194.

    Article  CAS  Google Scholar 

  15. Brüderlein, S., Gebhart, E., Siebert, E., and Augustus, M. (1987) Premature chromosome condensation studies on human metastatic carcinoma cells. Hum. Genet. 73, 44–52.

    Article  Google Scholar 

  16. Hittelman, W. N., Petkovic, I., and Agbor, P. (1988) Improvements in the premature chromosome condensation technique for cytogenetic analysis. Cancer Genet. Cytogenet. 30, 301–312.

    Article  CAS  PubMed  Google Scholar 

  17. Liehr, T., Grehl, H., and Rautenstrauss, B. (1996) A rapid method for FISH analysis of interphase nuclei extracted from cryofixed tissue. Trends Genet. 12, 505–506.

    Article  CAS  PubMed  Google Scholar 

  18. Speicher, M. R., Gwyn Ballard, S., and Ward, D. C. (1996) Karyotyping human chromosomes by combinatorial multi-fluor FISH. Nat. Genet. 12, 368–375.

    Article  CAS  PubMed  Google Scholar 

  19. Schröck, E., Veldman, T., Padilla-Nash, H., Ning, Y., Spurbeck, J., Jalal, S., et al. (1997) Spectral karyotyping refines cytogenetic diagnostics of constitutonal chromosomal abnormalities. Hum. Genet. 101, 255–262.

    Article  PubMed  Google Scholar 

  20. Chudoba, I., Plesch, A., Lörch, T., Claussen, U., and Senger, G. (1998) Multi-color-banding for the identification of interstitial deletion of chromosome 5. Medgen. 10, 116 (abstract CT-6).

    Google Scholar 

  21. Gebhart, E., Hofbeck, P., Hofmann, Y., Lerch, R., Schmitt, G., and Liehr, T. (1996) Recovering of archival tumor cytogenetic slides for two-color-interphase FISH. Appl. Cytogenet. 22, 146–148.

    Google Scholar 

  22. Gerdes, A. M., Pandis, N., Bomme, L., Dietrich, C. U., Teixeira, M. R., Bardi, G., and Heim, S. (1997) Fluorescence in situ hybridization of old G-banded and mounted chromosome preparations. Cancer Genet. Cytogenet. 98, 9–15.

    Article  CAS  PubMed  Google Scholar 

  23. Liehr, T., Atanasov, N., Tulusan, H. A., and Gebhart, E. (1993) Amplification of proto-oncogenes in human ovarian carcinomas. Int. J. Oncol. 2, 155–160.

    Google Scholar 

  24. Neubauer, S., Liehr, T., Tulusan, H. A., and Gebhart, E. (1994) Interphase cytogenetics by FISH on archival paraffin material and cultured cells of human ovarian tumors. Int. J. Oncol. 4, 317–321.

    Google Scholar 

  25. Liehr, T., Grehl, H., and Rautenstrauss, B. (1995) FISH analysis of interphase nuclei extracted from paraffin-embedded tissue. Trends Genet. 11, 377,378.

    Article  CAS  PubMed  Google Scholar 

  26. Wolfe, K. Q. and Herrington, C. S. (1997) Interphase cytogenetics and pathology: a tool for diagnostics and research. J. Pathol. 181, 359–361.

    Article  CAS  PubMed  Google Scholar 

  27. Koch, J. E., Kolvraa, S., Petersen, K. B., Gregersen, N., and Bolund, L. (1989) Oligonucleotide-priming methods for chromosome-specific labelling of alpha satellite DNA in situ. Chromosoma 98, 259–265.

    Article  CAS  Google Scholar 

  28. Werner, M., Nasarek, A., Tchinda, J., von Wasielewski, R., Komminooth, P., and Wilkens, L. (1997) Applications of single-color and double-color oligonucleotide primed in situ labelling in cytology. Mod. Pathol. 10, 1164–1171.

    CAS  PubMed  Google Scholar 

  29. Barlogie, B., Johnson, D. A., Smallwood, L., Raber, M. N, Maddox, A. M., Latreille, J., et al. (1982) Prognostic implications of ploidy and proliferative activity in human solid tumors. Cancer Genet. Cytogenet. 6, 17–28.

    Article  CAS  PubMed  Google Scholar 

  30. Persons, D. L., Hartmann, L. C., Herath, J. F., Borell, T. J., Keeney, G. L., and Jenkins, R. B., (1993) Interphase molecular cytogenetic analysis of epithelial ovarian tumors. Am. J. Clin. Pathol. 142, 733–741.

    CAS  Google Scholar 

  31. Gajewski, W. H., Fuller, A. F. Jr., Pastel-Ley, C., Flotte, T. J., and Bell, D. A. (1994) Prognostic significance of DNA content in epithelial ovarian cancer. Gynecol. Oncol. 53, 5–12.

    Article  CAS  PubMed  Google Scholar 

  32. Schueler, J. A., Trimbos, J. B., V. d. Burg, M., Cornelisse, C. J., Hermans, J., and Fleuren, G. J. (1996) DNA index reflects the biological behavior of ovarian carcinoma stage I-IIa. Gynecol. Oncol. 62, 59–66.

    Article  CAS  PubMed  Google Scholar 

  33. Abeln, E. C., Corver, W. E., Kuipers-Dijkshoorn, N. J., Fleuren, G. J., and Cornelisse, C. J. (1994) Molecular genetic analysis of flow-sorted ovarian tumor cells: improved detection of loss of heterozygosity. Br. J. Cancer 70, 255–262.

    Article  CAS  PubMed  Google Scholar 

  34. Kallioniemi, A., Kallioniemi, O. P., Sudar, D., Rutovitz, D., Gray, J. W., Waldman, F., and Pinkel, D. (1992) Comparative genomic hybridization for molecular cytogenetic analysis of solid tumors. Science 258, 818–821.

    Article  CAS  PubMed  Google Scholar 

  35. Speicher, M. R., du Manoir, S., Schröck, E., Holtgreve-Grez, H., Schoell, B., Lengauer, C., et al. (1993) Molecular cytogenetic analysis of formalin-fixed, paraffin-embedded solid tumors by comparative genomic hybridization after universal DNA-amplification. Hum. Mol. Genet. 2 1907–1914.

    Article  CAS  PubMed  Google Scholar 

  36. Kuukasjarvi, T., Tanner, M., Pennanen, S., Karhu, R., Visakorpi, T., and Isola, J. (1997) Optimizing DOP-PCR for universal amplification of small DNA samples in comparative genomic hybridization. Genes Chr. Cancer 18, 94–101.

    Article  CAS  Google Scholar 

  37. Tirkkonen, M., Karhu, R., Kallioniemi, O., and Isola, J. (1996) Evaluation of camera requirements for comparative genomic hybridization. Cytometry 25, 394–398.

    Article  CAS  PubMed  Google Scholar 

  38. Du Manoir, S., Schröck, E., Bentz, M., Speicher, M. R., Joos, S., Ried, T., et al. (1995) Quantitative analysis of comparative genomic hybridization. Cytometry 19, 27–41.

    Article  PubMed  Google Scholar 

  39. Karhu, R., Kahkonen, M., Kuukasjarvi, T., Pennanen, S., Tirkkonen, M., and Kallioniemi, O. (1997) Quality control of CGH: impact of metaphase chromosomes and the dynamic range of hybridization. Cytometry 28, 198–205.

    Article  CAS  PubMed  Google Scholar 

  40. Kallioniemi, O.-P., Kallioniemi, A., Piper, J., Isola, J., Waldman, F. M., Gray, J. W., and Pinkel, D. (1994) Optimizing comparative genomic hybridization for analysis of DNA sequence copy number changes in solid tumors. Genes Chr. Cancer 10, 231–243.

    Article  CAS  Google Scholar 

  41. Becher, R., Korn, W. M, and Prescher, G. (1997) Use of fluorescence in situ hybridization and comparative genomic hybridization in the cytogenetic analysis of testicular germ cell tumors and uveal melanomas. Cancer Genet Cytogenet. 93, 22–28.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut für Humangenetik, Jena, Germany

    Thomas Liehr

Authors
  1. Thomas Liehr
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Department of Surgery, University of Glasgow, Glasgow, Scotland

    John M. S. Bartlett

Rights and permissions

Reprints and permissions

Copyright information

© 2000 Humana Press Inc.

About this protocol

Cite this protocol

Liehr, T. (2000). Cytogenetics of Ovarian Cancer Technical Overview. In: Bartlett, J.M.S. (eds) Ovarian Cancer. Methods in Molecular Medicine™, vol 39. Humana Press. https://doi.org/10.1385/1-59259-071-3:207

Download citation

  • DOI: https://doi.org/10.1385/1-59259-071-3:207

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-583-6

  • Online ISBN: 978-1-59259-071-1

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation