Abstract
The tumor suppressor gene tp53 is mutated, deleted, or rearranged in more than 50% of human tumors (1). Wild-type (wt) tp53 stops growth and/or induces apoptosis in most transformed cells into which it is introduced, thus restricting research of such cells. One means of studying the effects of both wt and mutant tp53 is to generate cells in which tp53 activity can be experimentally manipulated using inducible transcriptional control elements to drive wt tp53 expression (2-4). Alternatively, temperature-sensitive (ts) tp53 mutants may be used. Such mutants were first analyzed by Oren (5) and possess wt tp53 activity at 32°C, but behave like other mutants tp53 molecules at 37°C.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Hollstein, M., Sidransky, D., Vogelstein, B., and Harris, C. C. (1991) p53 mutations in human cancers. Science 253, 49–53.
Shaw, P., Bovey, R., Tardy, S., Sahli, R., Sorda, B. T., and Costa, J. (1992) Induction of apoptosis by wild-type p53 in human colon tumor-derived cell line. Proc. Natl. Acad. Sci. USA 89, 4495–4499.
Lin, D., Shields, M. T., Ullrich, S. J., Appella, E., and Mercer, W. E. (1992) Growth arrest induced by wild-type p53 protein block cells prior to or near the restriction point near late G1 phase. Proc. Natl. Acad. Sci. USA 89, 9210–9214.
Agawal, M. L., Agawal, A., Taylor, W. R., and Stark, G. R. (1995) p53 controls both the G2/M and G1 cell cycle checkpoints and mediates revesible growth arrest in human fibroblasts. Proc. Natl. Acad. Sci. USA 92, 8493–8497.
Michalovitz, D., Halevy, O., and Oren, M. (1990) Conditional inhibition of transformation and of cell proliferation by temperature-sensitive mutant of p53. Cell, 62, 671–680.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2000 Humana Press Inc.
About this protocol
Cite this protocol
Vikhanskaya, F., Broggini, M. (2000). p53Transfectants in Ovarian Cancer. In: Bartlett, J.M.S. (eds) Ovarian Cancer. Methods in Molecular Medicine™, vol 39. Humana Press. https://doi.org/10.1385/1-59259-071-3:187
Download citation
DOI: https://doi.org/10.1385/1-59259-071-3:187
Publisher Name: Humana Press
Print ISBN: 978-0-89603-583-6
Online ISBN: 978-1-59259-071-1
eBook Packages: Springer Protocols