Abstract
Human tumor cell lines have provided valuable model systems to study a wide variety of tumor characteristics including the cell biology, genetics, and chemosensitivity profiles of disease. A large number of ovarian cancer cell lines have now been established and are in widespread use Table 1) (1-15). Many of these have been selected to reflect specific situations, e.g., pre- and postchemotherapy models or different histo- logical subtypes.
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References
Wolf, C. R., Hayward, I. P., Lawrie, S. S., Buckton, K., McIntyre, M. A., Adams, D. J., et al. (1987) Cellular heterogeneity and drug resistance in two ovarian adenocarcinoma cell lines derived from a single patient. Int. J. Cancer 39, 695–702.
Langdon, S. P., Lawrie, S. S., Hay, F. G., Hawkes, M. M., McDonald, A., Hayward, I. P., et al. (1988) Characterization and properties of nine human ovarian adenocarcinoma cell lines. Cancer Res. 48, 6166–6172.
Fogh, J., Fogh, J. M., and Orfeo, T. (1977) One hundred and twenty seven cultured tumour cell lines producing tumours in nude mice. J. Natl. Cancer Inst. 59, 221-226
Pirker, R., FitzGerald, D. J., Hamilton, T. C., Ozols, R. F., Laird, W., Frankel, A. E., et al. (1985) Characterization of immunotoxins active against ovarian cancer cell lines. J. Clin. Invest. 76, 1261–1267.
Hamilton, T. C., Young, R. C., McKoy, W. M., Grotzinger, K. R., Green, J. A., Chu, E. W., et al. (1983) Characterization of a human ovarian carcinoma cell line (NIH:OVCAR-3) with androgen and estrogen receptors. Cancer Res. 43, 5379–5389.
Louie, K. G., Behrens, B. C., Kinsella, T. J., Hamilton, T. C., Grotzinger, K. R., McKoy, W. M., et al. (1985) Radiation survival parameters of antineoplastic drug-sensitive and-resistant human ovarian cancer cell lines and their modification by buthionine sulfoximine. Cancer Res. 45, 2110–2115.
Louie, K. G., Hamilton, T. C., Winker, M. A., Behrens, B. C., Tsuruo, T., Klecker, R. W., et al. (1986) Adriamycin accumulation and metabolism in adriamycin-sensitive and-resistant human ovarian cancer cell lines. Biochem. Pharmacol. 35, 467–472.
Hills, C. A., Kelland, L. R., Abel, G., Siracky, J., Wilson, A. P., and Harrap, K. R. (1989) Biological properties of ten human ovarian carcinoma cell lines: calibration in vitro against four platinum complexes. Br. J. Cancer 59, 527–534.
Wilson, A. P., Dent, M., Pelovic, T., Hubbold, L., and Radford, H. (1996) Characterisation of seven human ovarian tumour cell lines. Br. J. Cancer 74, 722–727.
Buick, R. N., Pullano, R., and Trent, J. M. (1985) Comparative properties of five human ovarian adenocarcinoma cell lines. Cancer Res. 45, 3668–3676.
Woods, L. K., Morgan, R. T., Quinn, L. A., Moore, G. E., Semple, T. U., and Stedman, K. E. (1979) Comparison of four new cell lines from patients with adenocarcinoma of the ovary. Cancer Res. 39, 4449–459.
Benard, J., Da Silva, J., De Bois, M-C., Boyer, P., Duvillard, P., Chiric, E., et al. (1985) Characterization of a human ovarian adenocarcinoma line, IGROV1, in tissue culture and in nude mice. Cancer Res. 45, 4970–4979.
Ishiwata, I., Ishiwata, C., Soma, M., Nozawa, S., and Ishikawa, H. (1987) Characterization of newly established human ovarian carcinoma cell line-Special reference of the effects of cis-platinum on cellular proliferation and release of CA125. Gynecol. Oncol. 26, 340-354.
Horowitz, A. T., Treves, A. J., Voss, R., Okon, E., Fuks, Z., Davidson, L., et al. (1985) A new human ovarian carcinoma cell line: establishment and analysis of tumor-associated markers. Oncology 42, 332–337.
Briers, T. W., Stroobants, P., Vandeputte, T. M., Nouwen, E. J., Conraads, M. V., Eestermans, G., et al. (1989) Establishment and characterization of a human ovarian neoplastic cell line DO-s. Cancer Res. 49, 5153–5161.
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Langdon, S.P., Lawrie, S.S. (2000). Establishment of Ovarian Cancer Cell Lines. In: Bartlett, J.M.S. (eds) Ovarian Cancer. Methods in Molecular Medicine™, vol 39. Humana Press. https://doi.org/10.1385/1-59259-071-3:155
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DOI: https://doi.org/10.1385/1-59259-071-3:155
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