Abstract
The most important prognostic parameters for gynecologic malignancies are tumor stage, residual tumor after surgical treatment, histological subtype, and degree of malignancy (1-2). However, these factors present an incomplete picture of the tumor biology. Therefore, investigation of other prognostic factors is of special clinical relevance, particularly in view of the unexpectedly progressive course of the disease and frequent relapses in some cases.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Hacker, N. F., Berek, J. S., Lagasse, L. D., Nieberg, R. K., and Elashoff, R. M. (1983) Primary cytoreductive surgery for epithelial ovarian cancer. Obstet. Gynecol. 61, 413–420.
Malkasian, G. D. Jr., Melton, L. J., O’Brien, P. C., and Greene, M. H. (1984) Prognostic significance of histologic classification and grading of epithelial malignancies of the ovary. Am. J. Obstet. Gynecol. 149, 274–284.
Bishop, J. M. (1987) The molecular genetics of cancer. Science 235, 305–310.
Slamon, D. J., Godolphin, W., Jones, L. A., et al. (1989) Studies of the HER-2/neu proto-oncogene in human breast and ovarian cancer. Science 244, 707–712.
Slamon, D. J., Clark, G. M., Wong, S. G., Levin, W. J., Ullrich, A., and McGuire, W. L. (1987) Human breast cancer correlation of relapse and survival with amplification of the HER-2/neu oncogene. Science 235, 177–182.
Venter, D. J., Tuzi, N. L., Kumar, S., and Gullick, W. J. (1987) Overexpression of the c-erbB-2 oncoprotein in human breast carcinomas: immunohistological assessment correlation with gene amplification. Lancet 2, 68–72.
Berger, M. S., Locher, G. W., Saurer, S., Gullick, W. J., Waterfield, M. D., Groner, B., et al. (1988) Correlation of c-erbB-2 gene amplification and protein expression in human breast carcinoma with nodal status und nuclear grading. Cancer Res. 48, 1238–1243.
Marx, D., Schauer, A., Reiche, C., et al. (1990) C-erbB-2 expression in correlation to other biological parameters of breast cancer. J. Cancer Res. Clin. Oncol. 116, 15–20.
Berchuck, A., Kamel, A., and Whitaker, R. (1990) Overexpression of HER-2/neu is associated with poor survival in advanced epithelial ovarian cancer. Cancer Res. 50, 4087–4091.
Meden, H., Marx, D., Rath, W., Kron, M., Fattahi-Meibodi, A., Hinney, B., et al. (1994) Overexpression of the oncogene c-erbB-2 in primary ovarian cancer. Evaluation of the prognostic value in a Cox proportional hazards multiple regression. Int. J. Gynecol. Pathol. 13, 45–53.
Haldane, J. S., Hird, V., Hughes, C. M., and Gullick, W. J. (1990) C-erbB-2 oncogene expression in ovarian cancer. H. Pathol. 162, 231–237.
Zabrecky, J. R., Lam, T., McKenzie, S. J., and Carney, W. (1991) The extracellular domain of p185/neu is released from the surface of human breast carcinoma cells. SK-BR-3. J. Biol. Chem. 266, 1716–1720.
Meden, H., Marx, D., Fattahi, A., Rath, W., Kron, M., Wuttke, W., et al. (1997) Elevated serum levels of a c-erbB-2 oncogene product in ovarian cancer patients and in pregnancy. J. Cancer Res. Clin. Oncol. 129, 378–381.
Meden, H., Marx, D., Wuttke, W., Schauer, A., and Kuhn, W. (1997) Prognostic significance of p105 (c-erbB-2, HER2/neu) serum levels in patients with ovarian carcinoma. Anticancer Res. 17, 757–760.
Marx, D., Fattahi-Meibodi, A., Kudelka, R., Uebel, T., Kuhn, W., and Meden, H. (1998) p105 (c-erbB-2, HER2/neu) serum levels, detected by a new polyclonal antibody-based ELISA, in patients with ovarian carcinoma-correlation to patho-morphological results. Anticancer Res. 18, 2891–2894.
Mielke, S., Meden, H., Raab, T., Wuttke, W., and Kuhn, W. (1997) Effects of interfering and influ-encing factors of the analysis of p105 (c-erbB-2) oncoprotein fragment in serum. Anticancer Res. 17, 3125–3128.
Meden, H., Mielke, S., Marx, D., Wuttke, W., and Kuhn, W. (1997) Hormonal treatment with sex steroids in women is associated with lower p105 serum concentrations. Anticancer Res. 17, 757–760.
Meden, H., Mielke, S., Wuttke, W., and Kuhn, W. (1997) Elevated serum levels of the c-erbB-2 encoded oncoprotein fragment in cases of pure preeclampsia and HELLP syndrome. J. Obstet. Gynaecol. Res. 23, 213–217.
Roberts, J. M. and Redman, C. W. (1993) Pre-eclampsia: more than pregnancy-induced hypertension. Lancet 341, 1447–1451.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2000 Humana Press Inc.
About this protocol
Cite this protocol
Meden, H., Fattahi-Meibodi, A., Marx, D. (2000). ELISA-Based Quantification of p105 (c-erbB-2, HER2/neu) in Serum of Ovarian Carcinoma. In: Bartlett, J.M.S. (eds) Ovarian Cancer. Methods in Molecular Medicine™, vol 39. Humana Press. https://doi.org/10.1385/1-59259-071-3:125
Download citation
DOI: https://doi.org/10.1385/1-59259-071-3:125
Publisher Name: Humana Press
Print ISBN: 978-0-89603-583-6
Online ISBN: 978-1-59259-071-1
eBook Packages: Springer Protocols