Abstract
Certain immunological activities, particularly cell-mediated immunity, decline with advancing age. Gaining insight into the underlying mechanism(s) is complicated by the fact that human T-cells comprise several functionally and phenotypically distinct populations and subpopulations. Proliferative studies designed to identify differences between old and young subjects that are based entirely on unfractionated peripheral blood lymphocytes (PBLs) or pan T-cell responses run the risk, therefore, of missing subtle but perhaps crucial changes in a particular T-cell type.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Beckman, I., Shepherd, K., Dimopolous, K., Ahern, M., Firgaira, F., and Bradley, J. (1994) Differential regulation and expression of cytokine mRNA’s in human CD45R T cell subsets. Cytokine 6, 116–123.
Beckman, I., Shepard, K., Ahern, M., and Firgaira, F. (1995) Age-related defects in CD2-receptor induced activation in human T cell subsets. Immunology 86, 533–536.
Sewell, W. A., North, M. E., Webster, A. D., and Farrant, J. (1997) Determination of intracellular cytokines by flow-cytometry following whole blood culture. J. Immunol. Methods 10, 67–74.
Morita, Y., Yamamura, M., Kawashima, M., Harada, S., Tsuji, K., Shiuya, K., Maruyama, K., and Makino, H. (1998) Flow cytometric single cell analysis of cytokine production by CD4+ T cells in synovial tissue and peripheral blood from patients with rheumatoid arthritis. Arthritis Rheum. 41, 1669–1676.
Collins, D. P., Luebering, B. J., and Shaut, D. M. (1998) T-lymphocyte functionally assessed by analysis of cytokine receptor expression, intracellular cytokine expression, and femtomolar detection of cytokine secretion by quantitative flow cytometry. Cytometry 33, 249–255.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2000 Humana Press Inc., Totowa, NJ
About this protocol
Cite this protocol
Beckman, I. (2000). Assessment of T-Cell Function in the Aged. In: Barnett, Y.A., Barnett, C.R. (eds) Aging Methods and Protocols. Methods in Molecular Medicine, vol 38. Humana Press. https://doi.org/10.1385/1-59259-070-5:281
Download citation
DOI: https://doi.org/10.1385/1-59259-070-5:281
Publisher Name: Humana Press
Print ISBN: 978-0-89603-582-9
Online ISBN: 978-1-59259-070-4
eBook Packages: Springer Protocols