Abstract
There is accumulating evidence that oxidative damage to protein, lipid, carbohydrate and DNA is an important cause and/or effect of cellular and subcellular changes associated with disease, and is responsible for at least some of the physiological, but ultimately fatal, changes that accompany aging (1–8). Advancing age brings increasing risk of chronic degenerative disease including cancer, cardiovascular disease, cataracts, and dementia (1–6, 8).Immune status declines, with consequent increased risk of infection and, owing to a combination of physical and socioeconomic factors, nutritional status is often poor in the elderly, increasing the likelihood of poor antioxidant status (9).
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Notes
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References
Ame N., Shigenag K., and Hagen, T.M. (1993) Oxidants, antioxidants and the degenerative disease of aging.Proc.Nat.Acad.Sci.USA 90, 7915–7922.
Benzie, I.F.F. (1996) Lipid peroxidation:a review of causes, consequences, measurement and dietary influences.Int.J.Food Sci.Nutr. 47, 233–262.
Maxwell, S.R.J.and Lip, G.Y.H. (1997) Free radicals and antioxidants in cardiovascular disease.Br.J.Clin.Pharmacol. 44, 307–317.
Emerit, I. (1994) Reactive oxygen species, chromosome mutation and cancer:possible role of clastogenic factors in carcinogenesis.Free Radic.Biol.Med. 16, 99–109.
Yu, B.P. (1996) Aging and oxidative stress:modulation by dietary restriction.Free Radic.Biol.Med. 21, 651–668.
Harman, D. (1996) Aging and disease:extending functional lifespan.Ann.NY Acad. Sci. 786, 321–336.
Harman, D. (1956) Aging:a theory based on free radical and radiation chemistry. J.Gerontol. 2, 298–300.
Beckman, K.B.and Ames, B.N. (1998) The free radical theory of aging matures. Physiol.Rev. 78, 547–581.
Gariballa, S.E.and Sinclair, A.J. (1998) Nutrition, ageing and ill health.Br.J.Nutr. 80, 7–23.
Loft, S.and Poulson, H.E. (1996) Cancer risk and oxidative DNA damage in man. J.Mol.Med. 74, 297–312.
Duthie, S.J., Ma, A., Ross, M.A., and Collins, A.R. (1996) Antioxidant supplementation decreases oxidative damage in human lymphocytes.Cancer Res. 56, 1291–1295.
Heitzer, T., Just, H., and Munzel, T. (1996) Antioxidant vitamin C improves endothelial dysfunction in chronic smokers.Circulation 94, 6–9.
Levine, G.N., Frei, B., Koulouris, S.N., Gerhard, M.D., Keaney, J.F., and Vita, J.A. (1996) Ascorbic acid reverses endothelial vasomotor dysfunction in patients with coronary artery disease.Circulation 93, 1107–1113.
Ness, A.R., Chee, D., and Elliot, P. (1997) Vitamin C and blood pressure-an overview.J.Hum.Hyperten.11, 343–350.
Diplock, A.T. (1994) Antioxidants and disease prevention.Mol.Aspects Med. 15, 293–376.
Frei, B., Keaney, J.F., Retsky, K.L., and Chen, K. (1996) Vitamin C and E and LDL oxidation.Vitam.Horm. 54, 1–34.
Samman, S., Brown, A.J., Beltran, C., and Singh, S. (1997)The effect of ascorbic acid on plasma lipids and oxidisability of LDL in male smokers.Eur.J.Clin.Nutr. 51, 472–477.
Sweetman, S.F., Strain, J.J., and McKelvey, V.J. (1997) Effect of antioxidant vitamin supplementation on DNA damage and repair in human lymphoblastoid cell.Nutr.Cancer 27, 122–130.
Wen, Y., Cooke, T., and Feely, J. (1997) The effect of pharmacological supplementation with vitamin C on low-density lipoprotein oxidation.Br.J.Clin.Pharmacol. 44, 94–99.
Diplock, A.T., Charleux, J.L., Crozer-Willi, G., Kok, F.J., Rice-Evans, C., Roberfroid, M., Stahl, W.and Vina-Ribes, J. (1998) Functional food science and defence against reactive oxidative species.Br.J.Nutr. 80, S77–S112.
Bendich, A.and Langseth, L. (1995) The health effects of vitamin C supplementation J.Am.Coll.Nutr. 14, 124–136.
Benzie, I.F.F. (1998) Antioxidants:observational epidemiology, in The Encyclopedia of Nutrition (Sadler, M., Cabellero, B., and Strain, J.J., eds.), Academic Press London, pp. 106–115.
Halliwell, B. (1996) Oxidative stress, nutrition and health.Experimental strategies for optimization of nutritional antioxidant intake in humans.Free Radic.Res. 25, 57–74.
Machlin, L.J. (1995) Critical assessment of the epidemiological data concerning the impact of antioxidant nutrients on cancer and cardiovascular disease.Crit.Rev. Food Sci.Nutr. 35, 41–50.
Strain, J.J.and Benzie, I.F.F. (1998) Antioxidant nutrients, in Functional Foods; the Consumer, the Products and the Evidence (Sadler, M.J.and Saltmarsh, M., eds.), Royal Society of Chemistry Cambridge, pp. 74–79.
Weisburger, J.H. (1996) Tea antioxidants and health, in Handbook of Antioxidants (Cadenas, E.and Packer, L., eds.), Marcel Dekker New York, pp.469–486.
Benzie, I.F.F. (1999) Vitamin C:prospective functional markers for defining optimal nutritional status.Proc.Nutr.Soc., 58, 1–8.
Frei, B., England, L., and Ames, B.N. (1989) Ascorbate is an outstanding antioxidant in human blood plasma.Proc.Natl.Acad.Sci.USA 86, 6377–6381.
Rose, R.C.and Bode, A.M. (1993) Biology of free radical scavengers:an evaluation of ascorbate.FASEB J. 7, 1135–1142.
Traber, M.G.and Seis, H. (1996) Vitamin E in humans:demand and delivery. Annu.Rev.Nutr. 16, 321–347.
Strain, J.J.and Benzie, I.F.F. (1998) Diet and antioxidant defence, in The Encyclopedia of Nutrition (Sadler, M., Cabellero, B., and Strain, J.J., eds.), Academic Press London, pp. 95–105.
Sies, H.and Stahl, W. (1995) Vitamins E and C, B-carotene, and other carotenoids as antioxidants.Am.J.Clin.Nutr. 62, 1315S–1321S.
Graumlich, J.F., Ludden, T.M., Conry-Cantilena, C., Cantlena, L.R., Jr., Wang, Y., and Levine, M. (1997) Pharmacokinetic model of ascorbic acid in healthy male volunteers during depletion and repletion.Pharmacol.Res. 14, 1133–1139.
Gey, K.F. (1998) Vitamins E plus C and interacting conutrients required for optimal health.A critical and constructive review of epidemiology and supplementation data regarding cardiovascular disease and cancer.Biofactors 7, 113–174.
Benzie I.F.F.and Strain, J.J. (1996) The reducing ability of plasma as a measure of “antioxidant power ”-the FRAP assay. Anal.Biochem. 239, 70–76.
Benzie, I.F.F.and Strain, J.J. (1997) Simultaneous automated measurement of total antioxidant (reducing)capacity and ascorbic acid concentration.Redox Report 3, 233–238.
Benzie, I.F.F.and Strain, J.J. (1999) Ferric reducing (antioxidant) power as a measure of antioxidant capacity:the FRAP assay, in Methods in Enzymology, vol. 299: Oxidants and Antioxidants (Packer, L., ed.), Academic Press Orlando, pp. 15–27.
Benzie, I.F.F., Janus, E.D., and Strain, J.J. (1998) Plasma ascorbate and vitamin E levels in Hong Kong Chinese.Eur.J.Clin.Nutr. 52, 447–451.
Benzie, I.F.F.and Chung, W.Y. (1999) Total antioxidant power of plasma: male-female differences and effect of anticoagulant used.Ann.Clin.Biochem., 36, 104–106.
Benzie, I.F.F.and Szeto Y, T. (1999) Antioxidant power of teas.J.Agric.Food Chem., 47, 633–637.
Benzie, I.F.F.and Tomlinson, B. (1998) Antioxidant power of angiotensin-converting enzyme inhibitors in vitro.Br.J.Clin.Pharmacol.45, 168–169.
Benzie, I.F.F., Chung, W.Y., and Strain, J.J. (1999) Antioxidant activity of ascorbic acid is independent of concentration.J.Nutr.Biochem., 10, 146–150.
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Benzie, I.F.F., Strain, J.J. (2000). Assessing Age-Related Changes in Antioxidant Status. In: Barnett, Y.A., Barnett, C.R. (eds) Aging Methods and Protocols. Methods in Molecular Medicine, vol 38. Humana Press. https://doi.org/10.1385/1-59259-070-5:133
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DOI: https://doi.org/10.1385/1-59259-070-5:133
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