Abstract
Plant hormones are present and active in minor concentrations in plant tissue. To allow quantification in a restricted amount of material, i.e., protoplasts, chloroplasts, seedlings, seeds, buds, or apical root- and stem regions, a sensitive analytical technique is a prerequisite. During the last two decades, procedures for phytohormone analysis as well as the available hardware have improved substantially. Fluorimetry is widely used for the analysis of indole-3-acetic acid (IAA) because it is more specific and therefore more sensitive than ultraviolet detection (for a review see ref. 1). Good detection limits for derivatized IAA are obtained in Electron Impact (EI+) (2,3) or negative ion chemical ionization (NICI) GC-MS* (4,5).
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Prinsen, E., Van Laer, S., öden, S., Van Onckelen, H. (2000). Auxin Analysis. In: Tucker, G.A., Roberts, J.A. (eds) Plant Hormone Protocols. Methods in Molecular Biology™, vol 141. Humana Press. https://doi.org/10.1385/1-59259-067-5:49
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DOI: https://doi.org/10.1385/1-59259-067-5:49
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