Functional genomics has recently become a logistical rather than a theoretical problem in plant molecular biology for one simple reason—an immense wealth of data describing the nucleotide sequences of plant and animal genomes has become available and is continuing to accrue at an exponential rate. This has led to the development of a variety of methods under the general heading of functional genomics through which this ocean of information can be charted and given biological meaning with regard to the significance of individual nucleotide sequences. In order to assess the biological impact of individual genes within the context of a living organism, one might wish to investigate mutations or lesions in the gene of choice. Unfortunately, mutated lines are normally derived following selection based on an observation of phenotype, which then leads to an investigation of the molecular genetic changes underpinning the phenotypic changes observed. This forward genetic approach is very powerful, but does not lend itself readily to the investigation of genes without a known function (such as the analysis of theoretically predicted transcripts derived from genome sequencing projects).
KeywordsPolymerase Chain Reaction Polymerase Chain Reaction Amplification Reverse Genetic Positive Pool General Polymerase Chain Reaction
- 8.Fortsthoefel, N. R., Wu, Y., Schulz, B., Bennett, M. J., and Feldmann, K. A. (1992) T-DNA insertion mutagenesis in Arabidopsis: prospects and perspectives. Aust. J. Plant Physiol. 19, 353–366.Google Scholar
- 9.Arabidopsis Stock Centres:Eurasia:NASC,University of Nottingham,University Park,Nottingham,NG7 2RD,UK,NASC on-line catalogue.<http://nasc.nott.ac.uk/>.The Americas:ABRC, The Ohio State University,1735 Neil Avenue,309 Botany and Zoology Bldg.,Columbus,OH 43210,USA,ABRC on-line catalogue.< http://aims.cps.msu.edu/aims/>.
- 12.Old, R. W. and Primrose, S. B. (1996) Cloning Strategies, gene libraries, and cDNA cloning. In: Principles of Gene Manipulation: An Introduction to Genetic Engineering, 5th ed., Blackwell Scientific Publications, pp. 101,102.Google Scholar
- 16.May, S. T. (1998) Latin studies of plant architecture. Lab. News 576, 20–22.Google Scholar
- 17.Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual Vol I-III 2nd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.Google Scholar