Abstract
In this chapter, we describe the preparation and use of b-PG, a biotinylated complex that specifically binds hyaluronan (1,2). The b-PG is derived from cartilage and consists of a trypsin fragment of the proteoglycan core protein and one of the link proteins. Because of its ability to bind to hyaluronan with high affinity and specificity, the b-PG agent has proved to be useful in the histochemical localization of hyaluronan and its quantitative analysis by an enzyme linked assay (1,2).
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© 2000 Humana Press Inc., Totowa, NJ
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Underhill, C.B., Zhang, L. (2000). Analysis of Hyaluronan Using Biotinylated Hyaluronan-Binding Proteins. In: Tuan, R.S., Lo, C.W. (eds) Developmental Biology Protocols. Methods in Molecular Biology™, vol 137. Humana Press. https://doi.org/10.1385/1-59259-066-7:441
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DOI: https://doi.org/10.1385/1-59259-066-7:441
Publisher Name: Humana Press
Print ISBN: 978-0-89603-854-7
Online ISBN: 978-1-59259-066-7
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