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Cadherin-Mediated Cell-Cell Interactions

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Book cover Developmental Biology Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 137))

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Abstract

Cadherins constitute a family of transmembrane glycoproteins (Table 1) that mediate cell-cell adhesion by their ability to self-associate (1–4). For example, E-cadherin binds only to E-cadherin and not to N-cadherin. The homotypic interaction of cadherins is important for the sorting of cells during morphogenesis and for maintaining the normal structure and function of tissues (5–8). Cadherins are highly conserved across species and consist of three domains—i.e., an extracellular domain, transmembrane domain, and intracellular domain. The extracellular domain has multiple calcium-binding regions, which are required for cadherin function. The so-called classical cadherins have five extracellular subdomains (EC1-EC5) with the EC1 domain containing the sequences responsible for self-association and cell-cell adhesion. The intracellular domain is highly conserved across the cadherin family and associates with several proteins collectively termed catenins (9,10). The catenins mediate linkage of the cadherins to the actin cytoskeleton, a mechanism essential for optimal cadherin activity (11).

Table 1 Classical Cadherins and Other Selected Members of the Cadherin Family
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Authors and Affiliations

  1. Department of Biochemistry and Molecular Pharmacology,Lankenau Medical Research Center, Wynnewood, PA

    Karen A. Knudsen & Alejandro Peralta Soler

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  1. Karen A. Knudsen
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  2. Alejandro Peralta Soler
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Editors and Affiliations

  1. Thomas Jefferson University, Philadelphia, PA

    Rocky S. Tuan

  2. University of Pennsylvania, Philadelphia, PA

    Cecilia W. Lo

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© 2000 Humana Press Inc., Totowa, NJ

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Knudsen, K.A., Soler, A.P. (2000). Cadherin-Mediated Cell-Cell Interactions. In: Tuan, R.S., Lo, C.W. (eds) Developmental Biology Protocols. Methods in Molecular Biology™, vol 137. Humana Press. https://doi.org/10.1385/1-59259-066-7:409

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  • DOI: https://doi.org/10.1385/1-59259-066-7:409

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-854-7

  • Online ISBN: 978-1-59259-066-7

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