Abstract
The amphibian organizer corresponding to the upper dorsal blastopore lip of the early gastrula, is one of the classical systems in which embryonic patterning has been studied (1). Its two major inducing activities are neural induction and dorsalization of ventral mesoderm differentiation. Organizer activity is revealed either by transplantation of the upper dorsal blastopore lip into the ventral side of a host gastrula or by inserting the tissue into the gastrula blastocoel (Einsteck method). This results in the formation of an induced structure which, depending on the embryonic stage of the upper blastopore lip, is a head, trunk, or a tail. While the Einsteck is much easier to perform than the organizer transplant, the position of the implant can be less well controlled, which needs to be considered when interpreting the results (2). However, the Einsteck can be also carried out with heterologous material, cell pellets, and injected animal caps (3,4) and is more versatile than the organizer transplant.
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References
Lemaire, P. and Kodjabachian, L. (1996) The vertebrate organizer: structure and molecules. Trends Genet. 12, 525–531.
Slack, J. M. and Isaacs, H. V. (1994) The Einsteck-method: position and structure of projections formed by implants of a ventral character. Dev. Biol. 161, 313–317.
Ruiz i Altaba, A. and Melton, D. A. (1989) Interaction between peptide growth factors and homeobox genes in the establishment of antero-posterior polarity in frog embryos. Nature 341, 33–38.
Cho, K. W., Morita, E. A., Wright, C. V., and De, R. E. (1991) Overexpression of a homeodomain protein confers axis-forming activity to uncommitted Xenopus embryonic cells. Cell 65, 55–64.
Moon, R. T., Brown, J. D., Yang-Snyder, J. A., and Miller, J. R. (1997) Structurally related receptors and antagonists compete for secreted wnt ligands. Cell 88, 725–728.
Glinka, A., Wu, W., Onichtchouk, D., Blumenstock, C., and Niehrs, C. (1997) Head induction by simultaneous repression of Bmp and wnt signalling in Xenopus. Nature 389, 517–519.
Nieuwkoop, P. D. and Faber, J. (1967) Normal Table of Xenopus laevis. North Holland, Amsterdam, The Netherlands.
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© 2000 Humana Press Inc., Totowa, NJ
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Niehrs, C. (2000). Amphibian Organizer Activity. In: Tuan, R.S., Lo, C.W. (eds) Developmental Biology Protocols. Methods in Molecular Biology™, vol 137. Humana Press. https://doi.org/10.1385/1-59259-066-7:179
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DOI: https://doi.org/10.1385/1-59259-066-7:179
Publisher Name: Humana Press
Print ISBN: 978-0-89603-854-7
Online ISBN: 978-1-59259-066-7
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