Advertisement

Skeletogenesis

In Vitro Analysis of Bone Cell Differentiation
  • Adesola Majolagbe
  • Pamela Gehron Robey
Part of the Methods in Molecular Biology™ book series (MIMB, volume 137)

Abstract

A common goal of cell biologists is the establishment of in vitro model systems that faithfully recapitulate a particular biological process that occurs in vivo. Currently, numerous methodologies exist for in vitro analysis of osteoblastic cells, however, many of these methods depend on the use of fetal tissue, osteosarcoma, or immortalized cell lines. Although all of these model systems have generated a great deal of knowledge on the phenotypic character of osteoblastic cells as they undergo the maturational process that ultimately leads to the formation of a mineralized matrix, a great deal of variability has been noted in the literature from one culture method to another. This variability can arise from differences in the animal species and the developmental age of the starting material used for the establishment of such cultures, the amount of soft tissue associated with the starting material, and alterations in patterns of phenotypic expression owing to tumorogenic or immortalization processes (1).

Keywords

Calcium Chloride Osteoblastic Cell Bone Fragment Immortalize Cell Line Bone Chip 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Gehron Robey, P. (1992) Cell-mediated calcification in vitro, in Calcification in Biological Systems (Bonucci, E., ed.), CRC, Boca Raton, FL, pp. 107–127.Google Scholar
  2. 2.
    Gehron Robey, P. and Termine, J. D. (1985) Human bone cells in vitro. Calcif. Tissue Int. 37, 453–460.CrossRefGoogle Scholar
  3. 3.
    Fedarko, N. S., Termine, J. D., and Gehron Robey, P. (1990) High-performance liquid chromatographic separation of hyaluronan and four proteoglycans produced by human bone cell cultures. Anal. Biochem. 188, 398–407.PubMedCrossRefGoogle Scholar
  4. 4.
    Fedarko, N. S., Termine, J. D., Young, M. F., and Gehron Robey, P. (1990) Temporal regulation of hyaluronan and proteoglycan metabolism by human bone cells in vitro. J. Biol. Chem. 265, 12,200–12,209.PubMedGoogle Scholar
  5. 5.
    Fedarko, N. S., Bianco, P., Vetter, U., and Gehron Robey, P. (1990) Human bone cell enzyme expression and cellular heterogeneity: correlation of alkaline phosphatase enzyme activity with cell cycle. J. Cell Physiol. 144, 115–121.PubMedCrossRefGoogle Scholar
  6. 6.
    Fedarko, N. S., Vetter, U. K., Weinstein, S., and Gehron Robey, P. (1992) Age-related changes in hyaluronan, proteoglycan, collagen, and osteonectin synthesis by human bone cells. J. Cell Physiol. 151, 215–227.PubMedCrossRefGoogle Scholar
  7. 7.
    Gehron Robey, P. and Grzesik, W. J. (1995) The biochemistry of bone-forming cells: cell-matrix interactions, in Biological Mechanisms of Tooth Eruption, Resorption and Replacement by Implants (Davidovitch, Z., ed.), EBSCO Media, Birmingham, AL, pp. 167–172.Google Scholar
  8. 8.
    Lian, J. B. and Stein, G. S. (1995) Development of the osteoblast phenotype: molecular mechanisms mediating osteoblast growth and differentiation. Iowa Orthop. J. 15, 118–140.PubMedGoogle Scholar
  9. 9.
    Kasai, R., Bianco, P., Gehron Robey, P., and Kahn, A. J. (1994) Production and characterization of an antibody against the human bone GLA protein (BGP/osteocalcin) propeptide and its use in immunocytochemistry of bone cells. Bone Miner. 25, 167–182.PubMedCrossRefGoogle Scholar
  10. 10.
    Gehron Robey, P., Bianco, P., and Termine, J. D. (1992) The cell biology and molecular biochemistry of bone formation, in Disorders of Mineral Metabolism (Favus, M. J. and Coe, F. L., eds.), Raven, New York, pp. 241–263.Google Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 2000

Authors and Affiliations

  • Adesola Majolagbe
    • 1
  • Pamela Gehron Robey
    • 1
  1. 1.Craniofacial and Skeletal Diseases Branch, National Institute of Dental Research, National Institutes of HealthBethesda

Personalised recommendations