Abstract
During embryonic development, pluripotent cells from the endoderm layer are directed toward specific cell lineages, leading to the formation of highly specialized organs, such as liver, lung, and pancreas. In case of liver, hepatocyte specification and maturation is the result of a tissue interaction between prehepatic endoderm and adjacent pre-cardiac mesoderm and, subsequently, the mesenchyme of the septum transversum. During these interactions, molecules regulating hepatogenesis (“hepatogenic factors“) are presented to the endoderm by the mesoderm (1,2). In rat, the inductive potential of the mesoderm (hepatogenic activity) is highest until embryonic day (ED) 12 and subsequently fades to background level, reached at ED 15 (3).
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References
Le Douarin, N. M. (1975) An experimental analysis of liver development. Med. Biol. 53, 427–455.
Zaret, K. S. (1996) Molecular genetics of early liver development. Annu. Rev. Physiol. 58, 231–251.
Fukuda-Taira, S. (1981) Hepatic induction in the avian embryo: Specificity of reactive endoderm and inductive mesoderm. J. Embryol. Exp. Morphol. 63, 111–125.
Chenchik, A., Diatchenko, L., Chang, C., and Kuchibhatla, S. (1994) Great lengths cDNA synthesis kit for high yields of full-length cDNA. Clontechniques IX, 9–12.
Houssaint, E. (1976) Réversibilité de la dédifférenciation observée dans les cultures cellulaires de foie embryonnaire d’oiseau. J. Embryol. Exp. Morphol. 35, 227–240.
Chang, D. C., Chassy, B. M., Saunders, J. A., and Sowers, A. E. (1992) Guide to Electroporation and Electrofusion. Academic, San Diego, CA.
Fiers, W., Contreras, R., Haegeman, G., Rogiers, R., Voorde, A. van de, Heuverswyn, H. van, Herreweghe, J. van, Volckaert, G., and Ysebaert, M. (1978) Complete nucleotide sequence of SV40 DNA. Nature 273, 113–120.
Dingemanse, M. A., Boer, P. A. J. de, Moorman, A. F. M., Charles, R., and Lamers, W. H. (1994) The expression of liver-specific genes within rat embryonic hepatocytes is a discontinuous process. Differentiation 56, 153–162.
Horst, E., Wijngaard, P. L. J., Metzelaar, M., Bast, E. J. E. G., and Clevers, H. C. (1991) A method for cDNA cloning in COS cells irrespective of subcellular site of expression. Nucleic Acids Res. 19, 4556.
Hoff, M. J. B. van den, Moorman, A. F. M., and Lamers, W. H. (1992) Electroporation in “intracellular”buffer increases cell survival. Nucleic Acids Res. 20, 2902.
Melton, D. A., Krieg, P. A., Rebagliati, M. R., Maniatis, T., Zinn, K., and Green, M. R. (1984) Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promotor. Nucleic Acids Res. 12, 7035–7056.
Leffert, H. L. and Paul, D. (1972) Studies on primary cultures of differentiated fetal liver cells. J. Cell Biol. 52, 559–568.
Lamers, W. H., Zonneveld, D., and Charles, R. (1984) Inducibility of carbamoylphosphate synthetase (ammonia) in cultures of embryonic hepatocytes: Ontogenesis of the responsiveness to hormones. Dev. Biol. 105, 500–508.
Hoff, M. J. B. van den, Christoffels, V. M., Labruyere, W. T., Moorman, A. F. M., and Lamers, W. H. (1995) Electrotransfection with “intracellular”buffer, in Methods in Molecular Biology 48: Animal Cell Electroporation and Electrofusion Protocols. Humana, Totowa, NJ.
Gankema, H. S., Laanen, E., Groen, A. K., and Tager, J. M. (1981) Characterization of isolated rat-liver cells made permeable with filipin. Eur. J. Biochem. 119, 409–414.
Hoff, M. J. B. van den, Labruyere, W. T., Moorman, A. F. M., and Lamers, W. H. (1990) The osmolarity of the electroporation medium affects the transient expression of genes. Nucleic Acids Res. 18, 6464.
Jainchill, J. L., Aaronson, S. A., and Todaro, G. J. (1969) Murine sarcoma and leukemia viruses: assay using clonal lines of contact-inhibited mouse cells. J. Virol. 4, 549–553.
Sternberger, L. A. (1986) Immunocytochemistry, 3rd ed. Wiley, New York.
Graham, R. C., Jr., Lundholm, U., and Karnovsky, M. J. (1965) Cytochemical demonstration of peroxidase activity with 3-amino-9-ethylcarbazole. J. Histochem. Cytochem. 13, 150–152.
Hirt, B. (1967) Selective extraction of polyoma DNA from infected mouse cell cultures. J. Mol. Biol. 26, 365–369.
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Spijkers, J.A.A., Hakvoort, T.B.M., Lamers, W.H. (2000). Assay for the Isolation of Hepatogenic Factors. In: Tuan, R.S., Lo, C.W. (eds) Developmental Biology Protocols: Volume II. Methods in Molecular Biology™, vol 136. Humana Press. https://doi.org/10.1385/1-59259-065-9:87
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DOI: https://doi.org/10.1385/1-59259-065-9:87
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