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Gene-Targeting Strategies

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Developmental Biology Protocols: Volume II

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 136))

Abstract

Gene targeting in mouse embryonic stem (ES) cells has become a routine methodology to study gene function in vivo (1). Indeed, gene-targeting core facilities have been established at numerous institutions to facilitate the generation of targeted ES cell lines and the production of mouse chimeras. Although these facilities have centralized many of the unique skills and reagents that are required to generate “knockout”mice, the investigator must still generate a thoughtful strategy to mutate the gene of interest and to subsequently characterize the resulting mutant mice.

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Authors and Affiliations

  1. Department of Molecular Genetics, The University of Texas M.D.Anderson Cancer Center, Houston, TX

    Siew-Sim Cheah & Richard R. Behringer

Authors
  1. Siew-Sim Cheah
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  2. Richard R. Behringer
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Editor information

Editors and Affiliations

  1. Thomas Jefferson University, Philadelphia, PA

    Rocky S. Tuan

  2. University of Pennsylvania, Philadelphia, PA

    Cecilia W. Lo

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© 2000 Humana Press Inc.

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Cheah, SS., Behringer, R.R. (2000). Gene-Targeting Strategies. In: Tuan, R.S., Lo, C.W. (eds) Developmental Biology Protocols: Volume II. Methods in Molecular Biology™, vol 136. Humana Press. https://doi.org/10.1385/1-59259-065-9:455

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  • DOI: https://doi.org/10.1385/1-59259-065-9:455

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-853-0

  • Online ISBN: 978-1-59259-065-0

  • eBook Packages: Springer Protocols

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