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Production of Transgenic Mice with Yeast Artificial Chromosomes

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Developmental Biology Protocols: Volume II

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 136))

Abstract

The stable introduction of recombinant DNA into the germline of mammals is one of the major technological advances in biology and has greatly facilitated the study of gene function in development and disease,as well as the development of biotherapeutics. Landmark experiments conducted in the early 1980s established the feasibility and reproducibility of stably introducing DNA by microinjection into the male pronucleus of single-cell mouse embryos (1–5).The utilization of transgenic mice now represents a major strategy for the examination of genetic and biologic questions in vivo,both in basic research and industry.

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Author information

Authors and Affiliations

  1. UroGenesys,Inc., Santa Monica, CA

    Aya Jakobovits

  2. School of Medicine, Case Western Reserve University, Cleveland, OH

    Bruce T. Lamb

  3. Department of Biochemistry and Molecular Biology, University of Kansas Medical Center,School of Medicine, Kansas City, KS

    Kenneth R. Peterson

Authors
  1. Aya Jakobovits
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  2. Bruce T. Lamb
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  3. Kenneth R. Peterson
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Editors and Affiliations

  1. Thomas Jefferson University, Philadelphia, PA

    Rocky S. Tuan

  2. University of Pennsylvania, Philadelphia, PA

    Cecilia W. Lo

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Jakobovits, A., Lamb, B.T., Peterson, K.R. (2000). Production of Transgenic Mice with Yeast Artificial Chromosomes. In: Tuan, R.S., Lo, C.W. (eds) Developmental Biology Protocols: Volume II. Methods in Molecular Biology™, vol 136. Humana Press. https://doi.org/10.1385/1-59259-065-9:435

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  • DOI: https://doi.org/10.1385/1-59259-065-9:435

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-853-0

  • Online ISBN: 978-1-59259-065-0

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