Abstract
Ergot is a parasitic infection of cereal grains by the fungus Claviceps purpurea. The visible symptom of ergot is the presence of dark purple sclerotia (or “ergot bodies”) in place of the cereal kernel. Within these sclerotia the fungus produces ergot alkaloids as secondary metabolites (i.e., they do not contribute directly to the growth of the fungus). The production of ergot alkaloids is affected by many factors including the maturity of the fungus, its host plant, and geographic and prevailing weather conditions. Ergot bodies associated with rye, wheat, triticale, and barley have been found to contain up to 0.45%, 0.31%, 0.75%, and 1.04% of ergot alkaloids (1-3).
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References
Young, J. C. (1981) Variability in the content and composition of alkaloids found in Canadian ergot, I. Rye. J. Environ. Sci. Health 16B, 83–111.
Young, J. C. (1981) Variability in the content and composition of alkaloids found in Canadian ergot, II. Wheat. J. Environ. Sci. Health 16B, 381–393.
Young, J. C. (1982) Variability in the content and composition of alkaloids found in Canadian ergot, III. Triticale and barley, J. Environ. Sci. Health 17B, 93–107.
Flieger, M., Wurst, M., and Shelby, R. (1997) Ergot alkaloids-sources, structures and analytical methods. Folia Microbiol. 42, 3–30..
Scott, P. M. and Lawrence, G. A. (1980) Analysis of ergot alkaloids in flour. J. Ag. Food Chem. 28, 1258–1261.
Scott, P. M., Lombaert, G. A., Pellaers, P., Bacler, S., and Lappi, J. (1992) Ergot alkaloids in grain foods sold in Canada. J. AOAC Int. 75, 773–779.
Yates, S. G. and Powell, R. G. (1988) Analysis of ergopeptine alkaloids in endo-phyte infected tall fescue. J. Ag. Food Chem. 36, 337–340.
Fajardo, J. E., Dexter, J. E., Roscoe, M. M., and Nowicki, T. W. (1995) Retention of ergot alkaloids in wheat during processing, Cereal Chem. 72, 291–298.
Edlund, P. O. (1981) Determination of ergot alkaloids in plasma by high-performance liquid chromatography and fluoresence detection. J. Chromatogr. 226, 107–115.
Chervet, J. P. and Plas, D. (1984) Fast separation of some ergot alkaloids by high-performance liquid chromatography. J. Chromatogr. 295, 282–290.
Porter, J. K. and Betowski, D. (1981) Chemical ionization mass spectrometry of ergot cyclol alkaloids, J. Ag. Food Chem. 29, 650–653.
Yates, S. G., Plattner, R. D., and Garner, G. B. (1985) Detection of ergopeptine alkaloids in endophyte infected, toxic Ky-31 tall fescue by mass spectrometry/ mass spectrometry. J. Ag. Food Chem. 33, 719–722.
Shelby, R. A., Olsovska, J., Havlicek, V., and Flieger, M. (1997) Analysis of ergot alkaloids in endophyte infected tall fescue by liquid chromatography/ electrospray mass spectrometry. J. Ag. Food Chem. 45, 4674–4679
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© 2001 Humana Press Inc.
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Lombaert, G.A. (2001). Liquid Chromatographic Method for the Determination of Ergot Alkaloids in Cereal Grains. In: Trucksess, M.W., Pohland, A.E. (eds) Mycotoxin Protocols. Methods in Molecular Biology™, vol 157. Humana Press. https://doi.org/10.1385/1-59259-064-0:215
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DOI: https://doi.org/10.1385/1-59259-064-0:215
Publisher Name: Humana Press
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