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Liquid Chromatographic Method for Fumonisins in Corn

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Book cover Mycotoxin Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 157))

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Abstract

The fumonisins are an economically important group of mycotoxins that occur worldwide in corn and corn-based products. They are produced by a number of Fusarium species, of which F. moniliforme and F. proliferatum are the most common, as they infect corn crops around the world (1). Although a number of fumonisins have been identified (2), the most abundant analogs found in naturally contaminated food and feeds are fumonisin B1 (FB1), B2 (FB2), and B3 (FB3) (3). Analytical determination of these mycotoxins has shown them to be almost universally present in corn and has heightened concern over the implications that ingestion of these toxins has for animal and human health (3). Of particular concern, is the reported co-occurrence in corn of fumonisins, which have been shown to have cancer promoting properties (4), with the known human carcinogen, aflatoxin (5–8). In animals, fumonisins are the causative agents of the fatal syndromes, leukoencephalomalacia in horses (9) and pulmonary edema and hydrothorax in pigs (10). They produce nephrotoxicity (11) and hepatocarcinoma (12) in rats and are toxic to turkey poults (13) and broiler chicks (14). Fumonisins have been shown to cause developmental toxicity, including fetal deaths and fetal resorptions, in hamsters (15), mice (16) and rats (17). However, experimental evidence suggests that for mice and rats, these effects may be mediated by maternal toxicity (16,17).

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References

  1. Marasas, W. F. O., Nelson, P. E., and Toussoun, T. A. (1984) in Toxigenic Fusarium Species Identity and Mycotoxicology, Pennsylvania State University Press, University Park, PA, pp. 216–246.

    Google Scholar 

  2. Musser, S. M. and Plattner, R. D. (1997) Fumonisin composition in cultures of Fusarium moniliforme, Fusarium proliferatum and Fusarium nygami. J. Agric. Food Chem. 45, 1169–1173.

    Article  CAS  Google Scholar 

  3. Shephard, G. S., Thiel, P. G., Stockenström, S., and Sydenham, E. W. (1996) Worldwide survey of fumonisin contamination of corn and corn-based products. J.AOACInt. 79, 671–687. 155

    CAS  Google Scholar 

  4. Gelderblom, W. C. A., Snyman, S. D., Lebepe-Mazur, S., Van der Westhuizen, L., Kriek, N. P. J., and Marasas, W. F. O. (1996) The cancer-promoting potential of fumonisin B1 in rat liver using diethylnitrosamine as a cancer initiator. Cancer Letters 109, 101–108.

    Article  PubMed  CAS  Google Scholar 

  5. Chamberlain, W. J., Bacon, C. W., Norred, W. P., and Voss, K. A. (1993) Levels of fumonisin B1 in corn naturally contaminated with aflatoxins. Food Chem. Toxicol. 31, 995–998.

    Article  PubMed  CAS  Google Scholar 

  6. Ueno, Y., Iijima, K., Wang, S.-D., Sugiura, Y., Sekijima, M., Tanaka, T., Chen, C., and Yu, S.-Z. (1997) Fumonisins as a possible contributory risk factor for primary liver cancer: A 3-year study of corn harvested in Haimen, China, by HPLC andELISA. Food Chem. Toxicol. 35, 1143–1150.

    Article  PubMed  CAS  Google Scholar 

  7. Shetty, P. H. and Bhat, R. V. (1997) Natural occurrence of fumonisin B1 and its co-occurrence with aflatoxin B1 in Indian sorghum, maize and poultry feeds. J. Agric. Food Chem. 45, 2170–2173.

    Article  CAS  Google Scholar 

  8. Yamashita, A., Yoshizawa, T., Aiura, Y., Sanchez, P. C., Dizon, E. I., Arim, R. H., and Sardjono (1995) Fusarium mycotoxins (fumonisins, nivalenol and zearalenone) and aflatoxins in corn from southeast Asia. Biosci. Biotech. Biochem. 59, 1804–1807.

    Article  CAS  Google Scholar 

  9. Kellerman, T. S., Marasas, W. F. O., Thiel, P. G., Gelderblom, W. C. A., Cawood, M., and Coetzer, J. A. W. (1990) Leukoencephalomalacia in two horses induced by oral dosing of fumonisin B1. Onderstepoort J. Vet. Res. 57, 269–275.

    PubMed  CAS  Google Scholar 

  10. Harrison, L. R., Colvin, B. M., Green, J. T., Newman, L. E., and Cole, J. R. (1990) Pulmonary edema and hydrothorax in swine produced by fumonisin B1, a toxic metabolite of Fusarium moniliforme. J. Vet. Diagn. Invest. 2, 217–221.

    PubMed  CAS  Google Scholar 

  11. Riley, R. T., Hinton, D. M., Chamberlain, W. J., Bacon, C. W., Wang, E., Merrill, A. H., Jr., and Voss, K. A. (1994) Dietary fumonisin B1 induces disruption of sphingolipid metabolism in Sprague-Dawley rats: a new mechanism of nephro-toxicity. J. Nutr. 124, 594–603.

    PubMed  CAS  Google Scholar 

  12. Gelderblom, W. C. A., Kriek, N. P. J., Marasas, W. F. O., and Thiel, P. G. (1991) Toxicity and carcinogenicity of the Fusarium moniliforme metabolite, fumonisin B1, in rats. Carcinogenesis 12, 1247–1251.

    Article  PubMed  CAS  Google Scholar 

  13. Weibking, T. S., Ledoux, D. R., Brown, T. P., and Rottinghaus, G. E. (1993) Fumonisin toxicity in turkey poults. J. Vet. Diagn. Invest. 5, 75–83.

    PubMed  CAS  Google Scholar 

  14. Weibking, T. S., Ledoux, D. R., Bermudez, A. J., Turk, J. R., Rottinghaus, G. E., Wang, E., and Merrill, A. H., Jr. (1993) Effects of feeding Fusarium moniliformeculture material, containing known levels of fumonisin B1, on the young broiler chick. Poultry Sci. 72, 456–466.

    CAS  Google Scholar 

  15. Penner, J. D., Casteel, S. W., Pittman, L., Rottinghaus, G. E., and Wyatt, R. D. (1998) Developmental toxicity of purified fumonisin B1 in pregnant Syrian hamsters. J. Appl. Toxicol. 18, 197–203.

    Article  PubMed  CAS  Google Scholar 

  16. Reddy, R. V., Johnson, G., Rottinghaus, G. E., Casteel, S. W., and Reddy, C. S. (1996) Developmental effects of fumonisin B1 in mice. Mycopathologia 134, 161–166.

    Article  PubMed  CAS  Google Scholar 

  17. Collins, T.F.X., Sprando, R.L., Black, T.N., Shackelford, M.E., Laborde, J.B., Hansen, D.K., Eppley, R.M., Trucksess, M.W., Howard, P.C., Bryant, M.A., Ruggles, D. I., Olejnik, N., and Rorie, J. I. (1998) Effects of fumonisin B1 in pregnant rats. Part 2. Food Chem. Toxicol. 36, 673–685.

    Article  PubMed  CAS  Google Scholar 

  18. Rheeder, J. P., Marasas, W. F. O., Thiel, P. G., Sydenham, E. W., Shephard, G. S., and Van Schalkwyk, D. J. (1992) Fusarium moniliforme and fumonisins in corn in relation to human esophageal cancer in Transkei. Phytopathology 82, 353–357.

    Article  Google Scholar 

  19. Chu, F. S. and Li, G. Y. (1994) Simultaneous occurrence of fumonisin B1 and other mycotoxins in moldy corn collected from the People’s Republic of China in regions with high incidences of esophageal cancer. Appl. Environ. Microbiol. 60, 847–852.

    PubMed  CAS  Google Scholar 

  20. Zhang, H., Nagashima, H., and Goto, T. (1997) Natural occurrence of mycotoxins in corn, samples from high and low risk areas for human esophageal cancer in China. Mycotoxins 44, 29–35.

    Google Scholar 

  21. Doko, M. B. and Visconti, A. (1994) Occurrence of fumonisins B1 and B2 in corn and corn-based human foodstuffs in Italy. FoodAddit. Contam. 11, 433–439.

    CAS  Google Scholar 

  22. Vainio, H., Heseltine, E., and Wilbourn, J. (1993) Report on a IARC working group meeting on some naturally occurring substances. Int. J. Cancer 53, 535–537.

    Article  PubMed  CAS  Google Scholar 

  23. Shier, W. T., Abbas, H. K., and Mirocha, C. J. (1991) Toxicity of the mycotoxins fumonisins B1 and B2 and Alternaria alternata f. sp. lycopersici toxin (AAL) in cultured mammalian cells. Mycopathologia 116, 97–104.

    Article  PubMed  CAS  Google Scholar 

  24. Tolleson, W. H., Melchior, W. B., Jr., Morris, S. M., McGarrity, L. J., Domon, O. E., Muskhelishvili, L., James, S. J., and Howard, P. C. (1996) Apoptotic and anti-proliferative effects of fumonisin B1 in human keratinocytes, fibroblasts, esophageal epithelial cells and hepatoma cells. Carcinogenesis 17, 239–249.

    Article  PubMed  CAS  Google Scholar 

  25. Tolleson, W. H., Dooley, K. L., Sheldon, W. G., Thurman, J. D., Bucci, T. J., and Howard, P. C. (1996) The mycotoxin fumonisin induces apoptosis in cultured human cells and in livers and kidneys of rats. Adv. Exp. Med. Biol. 392, 237–250.

    PubMed  CAS  Google Scholar 

  26. Wang, E., Norred, W. P., Bacon, C. W., Riley, R. T., and Merrill, A. H., Jr. (1991) Inhibition of sphingolipid biosynthesis by fumonisins-implications for diseases associated with Fusarium moniliforme. J. Biol. Chem. 266, 14,486–14,490.

    PubMed  CAS  Google Scholar 

  27. Shephard, G. S., Van der Westhuizen, L., Thiel, P. G., Gelderblom, W. C. A., Marasas, W. F. O., and Van Schalkwyk, D. J. (1996) Disruption of sphingolipid metabolism in non-human primates consuming diets of fumonisin-containing Fusarium moniliforme culture material. Toxicon 34, 527–534.

    Article  PubMed  CAS  Google Scholar 

  28. Wang, E., Ross, P. F., Wilson, T. M., Riley, R. T., and Merrill, A. H., Jr. (1992) Increases in serum sphingosine and sphinganine and decreases in complex sphingolipids in ponies given feed containing fumonisins, mycotoxins produced by Fusarium moniliforme. J. Nutr. 122, 1706–1716.

    PubMed  CAS  Google Scholar 

  29. Riley, R. T., Wang, E., Schroeder, J. J., Smith, E. R., Plattner, R. D., Abbas, H., Yoo, H.-S., and Merrill, A. H., Jr. (1996) Evidence for disruption of sphingolipid metabolism as a contributory factor in the toxicity and carcinogenicity of fumonisins. Nat. Toxins 4, 3–15.

    Article  PubMed  CAS  Google Scholar 

  30. Gelderblom, W. C. A., Snyman, S. D., Van der Westhuizen, L., and Marasas, W. F. O. (1995) Mitoinhibitory effect of fumonisin B1 on the EGF-induced mitogenic response in primary rat hepatocytes. Carcinogenesis 16, 625–631. 157

    Article  PubMed  CAS  Google Scholar 

  31. Gelderblom, W. C. A., Smuts, C. M., Abel, S., Snyman, S. D., Van der Westhuizen, L., Huber, W. W., and Swanevelder, S. (1997) Effect of fumonisin B1 on the levels and fatty acid composition of selected lipids in rat liver in vivo. Food Chem. Toxicol. 35, 647–656.

    Article  PubMed  CAS  Google Scholar 

  32. Ciacci-Zanella, J. R., Merrill, A. H., Jr., Wang, E., and Jones, C. (1997) The mycotoxin fumonisin B1 induces CDKI (cyclin dependent kinase inhibitors) in CV-1 cells. Proceedings of the 88th Annual Meeting of American Association for Cancer Research, 12-16 April, San Diego, CA, 38, Abst. #3327, p. 496.

    Google Scholar 

  33. Ramljak, D., Diwan, B. A., Ramakrishna, G., Victor, T. C., Marasas, W. F. O., Anderson, L. M., and Gelderblom, W. C. A. (1997) Overexpression of cyclin D1 is an early event in, and possible mechanism responsible for, fumonisin B1 liver tumorigenesis in rats. Proceedings of the 88th Annual Meeting of American Association for Cancer Research, 12-16 April, San Diego, CA, 38, Abst. #3316, p. 495.

    Google Scholar 

  34. Report of Joint Mycotoxin Committee (1994) J. AOAC Int. 77, 257–258.

    Google Scholar 

  35. Food and Agricultural Organization (FAO) (1997) Worldwide regulations for mycotoxins 1995. A compendium. FAO Food and Nutrition Paper 64. Food and Agricultural Organization of the United Nations, Rome, Italy.

    Google Scholar 

  36. Marasas, W. F. O. (1997) Risk assessment of fumonisins produced by Fusarium moniliforme in corn. Cereal Res. Commun. 25, 399–406.

    CAS  Google Scholar 

  37. Martinova, E. A. and Merrill, A. H., Jr. (1995) Fumonisin B1 alters sphingolipid metabolism and immune function in BALB/c mice: Immunological responses to fumonisin B1. Mycopathologia 130, 163–170.

    Article  PubMed  CAS  Google Scholar 

  38. Miller, J. D. (1998) Global significance of mycotoxins, in Mycotoxins and Phycotoxins-Developments in Chemistry, Toxicology and Food Safety (Miraglia, M., Van Egmond, H., Brera, C., and Gilbert, J., eds.), Proceedings of the 9th International IUPAC Symposium on Mycotoxins and Phycotoxins, Rome, Italy, 27-31 May 1996, Alaken Inc., Fort Collins, CO, pp. 3–15.

    Google Scholar 

  39. Sydenham, E. W., Shephard, G. S., Thiel, P. G., Stockenström, S., and Van Schalkwyk, D. J. (1996) Liquid chromatographic determination of fumonisins B1, B2 and B3 in corn: AOAC-IUPAC collaborative study. J. AOAC Int. 79, 688–696.

    PubMed  CAS  Google Scholar 

  40. Thiel, P. G., Sydenham, E. W., Shephard, G. S., and Van Schalkwyk, D. J. (1993) Study of the reproducibility characteristics of a liquid chromatographic method for the determination of fumonisins B1 and B2 in corn: IUPAC collaborative study. J. AOAC Int. 76, 361–366.

    CAS  Google Scholar 

  41. Scott, P. M. and Lawrence, G. A. (1994) Stability and problems in recovery of fumonisins added to corn-based foods. J. AOAC Int. 77, 541–545.

    PubMed  CAS  Google Scholar 

  42. Bennett, G. A. and Richard, J. L. (1994) Liquid chromatographic method for the analysis of the naphthalene dicarboxaldehyde derivative of fumonisin. J. AOAC Int. 77, 501–506.

    CAS  Google Scholar 

  43. Sydenham, E. W., Shephard, G. S., and Thiel, P. G. (1992) Liquid chromatographic determination of fumonisins B1, B2 and B3 in foods and feeds. J. AOAC Int. 75, 313–318.

    CAS  Google Scholar 

  44. Wilson, T. M., Ross, P. F., Rice, L. G., Osweiler, G. D., Nelson, H. A., Owens, D. L., Plattner, R. D., Reggiardo, C., Noon, T. H., and Pickrell, J. W. (1990) Fumonisin B1 levels associated with an epizootic of equine leukoencephalo-malacia. J. Vet. Diagn. Invest. 2, 213–216.

    PubMed  CAS  Google Scholar 

  45. Rice, L. G., Ross, P. F., Dejong, J., Plattner, R. D., and Coats, J. R. (1995) Evaluation of a liquid chromatographic method for the determination of fumonisins in corn, poultry feed and Fusarium culture material. J. AOAC Int. 78, 1002–1009.

    PubMed  CAS  Google Scholar 

  46. Visconti, A., Boenke, A., Solfrizzo, M., Pascale, M., and Doko, M. B. (1996) European intercomparison study for the determination of the fumonisins content in two maize materials. FoodAddit. Contam. 13, 909–927.

    CAS  Google Scholar 

  47. Stockenström, S., Sydenham, E. W., and Thiel, P. G. (1994) Determination of fumonisins in corn: Evaluation of two purification procedures. Mycotoxin Res. 10, 9–14.

    Article  Google Scholar 

  48. Ware, G. M., Umrigar, P. P., Carman, A. S., Jr., and Kuan, S. S. (1994) Evaluation of fumonitest immunoaffinity columns. Anal. Lett. 27, 693–715.

    CAS  Google Scholar 

  49. Duncan, K., Kruger, S., Zabe, N., Kohn, B., and Prioli, R. (1998) Improved fluo-rometric and chromatographic methods for the quantification of fumonisins B1, B2 and B3. J. Chromatogr. A 815, 41–47.

    Article  PubMed  CAS  Google Scholar 

  50. Churchwell, M. I., Cooper, W. M., Howard, P. C., and Doerge, D. R. (1997) Determination of fumonisins in rodent feed using HPLC with electrospray mass spectrometry detection. J. Agric. Food Chem. 45, 2573–2578.

    Article  CAS  Google Scholar 

  51. Lukacs, Z., Schaper, S., Herderich, M., Schreier, P., and Humpf, H.-U. (1996) Identification and determination of fumonisin FB 1 and FB2 in corn and corn products by high performance liquid chromatography-electrospray-ionization tandem mass spectrometry (HPLC-ESI-MS-MS). Chromatographia 43, 124–128.

    Article  CAS  Google Scholar 

  52. Plattner, R. D. (1995) Detection of fumonisins produced in Fusarium moniliformecultures by HPLC with electrospray MS and evaporative light scattering detectors. Nat. Toxins 3, 294–298.

    Article  PubMed  CAS  Google Scholar 

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  1. PROMEC, Medical Research Council, Tygerberg, South Africa

    Gordon S. Shephard

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  1. Food and Drug Administration, Washington, DC

    Mary W. Trucksess  & Albert E. Pohland  & 

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Shephard, G.S. (2001). Liquid Chromatographic Method for Fumonisins in Corn. In: Trucksess, M.W., Pohland, A.E. (eds) Mycotoxin Protocols. Methods in Molecular Biology™, vol 157. Humana Press. https://doi.org/10.1385/1-59259-064-0:147

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  • DOI: https://doi.org/10.1385/1-59259-064-0:147

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