16 Immunochemical Method for Citrinin

  • David Abramson
  • Ewald Usleber
  • Erwin Märtlbauer
Part of the Methods in Molecular Biology™ book series (MIMB, volume 157)


Citrinin is a toxic metabolite produced by several species of Aspergillus and Penicillium (1), and was originally isolated as an experimental antibiotic from fermentation cultures of Penicillium citrinum in 1931 (2). Even today, liquid cultures of toxigenic Penicillia, such as P. citrinum NRRL-5907 or NRRL-1471, are still the most economical sources for fractional-gram quantities of nearly pure citrinin. A single precipitation from hot ethanol as described (2) affords crystalline material which is homogeneous when assayed by fluorescence high-performance liquid chromatography (HPLC).


Sodium Chloride Keyhole Limpet Hemocyanin Aluminum Hydride Potassium Citrate Lithium Aluminum Hydride 
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Copyright information

© Humana Press Inc. 2001

Authors and Affiliations

  • David Abramson
    • 1
  • Ewald Usleber
    • 2
  • Erwin Märtlbauer
  1. 1.Cereal Research CentreAgriculture and Agri-Food CanadaWinnipegCanada
  2. 2.Institute for Hygiene and Technology of Food of Animal OriginUniversity of MunichGermany

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