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Extracellular Matrix Protocols pp 59–69Cite as

Preparation of Isotopically Labeled Recombinant Fragments of Fibronectin for Functional and Structural Study by Heteronuclear Nuclear Magnetic Resonance Spectroscopy

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 139))

Abstract

The collagens, proteoglycans, and glycoproteins of the extracellular matrix (ECM) are a diverse collection of macromolecules, heterogeneous in size, structure, and function. However, their primary structures have revealed that their polypeptide components are frequently comprised of a series of sequence repeats or “modules” (1). This mosaic nature of ECM macromolecules permits their properties to be analyzed by a “dissection” strategy (2), where protein fragments generated by proteolysis, chemical synthesis, or recombinant expression are employed in structural and functional investigations.

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Author information

Authors and Affiliations

  1. Department of Biochemistry, University of Oxford, Oxford, UK

    Jeremy R. Bright, Andrew R. Pickford, Jennifer R. Potts & Iain D. Campbell

Authors
  1. Jeremy R. Bright
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  2. Andrew R. Pickford
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  3. Jennifer R. Potts
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  4. Iain D. Campbell
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Editor information

Editors and Affiliations

  1. The Wellcome Trust Centre for Cell-Matrix Research, School of Biological Sciences, University of Manchester, Manchester, UK

    Charles H. Streuli  & Michael E. Grant  & 

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© 2000 Humana Press Inc.

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Bright, J.R., Pickford, A.R., Potts, J.R., Campbell, I.D. (2000). Preparation of Isotopically Labeled Recombinant Fragments of Fibronectin for Functional and Structural Study by Heteronuclear Nuclear Magnetic Resonance Spectroscopy. In: Streuli, C.H., Grant, M.E. (eds) Extracellular Matrix Protocols. Methods in Molecular Biology™, vol 139. Humana Press. https://doi.org/10.1385/1-59259-063-2:59

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  • DOI: https://doi.org/10.1385/1-59259-063-2:59

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-624-6

  • Online ISBN: 978-1-59259-063-6

  • eBook Packages: Springer Protocols

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