Abstract
This chapter will outline in detail the two standard assays used in the author’ s laboratory for quantitating the adhesion of cells to an immobilized substrate. The attachment assay, which employs a colorimetric detection of bound cells, is based on Kueng et al. (1), and the spreading assay, which employs phase contrast microscopy to measure the flattening of adherent cells, is based on the method of Yamada and Kennedy (2).
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References
Kueng, W., Silber, E., and Eppenberger, U. (1989) Quantification of cells cultured on 96-well plates. Anal. Biochem. 182, 16–19.
Yamada, K. M. and Kennedy, D. W. (1984) Dualistic nature of adhesive protein function: fibronectin and its biologically active peptide fragments can autoinhibit fibronectin function. J. Cell Biol. 99, 29–36.
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© 2000 Humana Press Inc.
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Humphries, M.J. (2000). Cell Adhesion Assays. In: Streuli, C.H., Grant, M.E. (eds) Extracellular Matrix Protocols. Methods in Molecular Biology™, vol 139. Humana Press. https://doi.org/10.1385/1-59259-063-2:279
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DOI: https://doi.org/10.1385/1-59259-063-2:279
Publisher Name: Humana Press
Print ISBN: 978-0-89603-624-6
Online ISBN: 978-1-59259-063-6
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