Abstract
The insulin-like growth factor-binding proteins (IGFBPs) are carrier proteins that are present in all extracellular fluids (1). Similarly, insulin-like growth factor-I (IGF-I) and -II are synthesized by connective tissue cells that are present within the stroma of all tissues, and therefore their presence is ubiquitous in all organs (2). IGF receptors are present in all tissues, which accounts for balanced growth of organs, and deletion of the IGF-I gene results in a balanced decrease in organ growth, whereas overexpression of IGF-I results in a symmetric increase in growth (3).
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Reference
Jones, J. I. and Clemmons, D. R. (1995) Insulin like growth factor and their binding proteins: biologic actions. Endocrine. Rev. 16, 3–34.
Han, V. K. M., D’Ercole, A. J., and Lund, P. K. (1987) Cellular location of somatomedin (insulin-like growth factor) messenger RNA in the human fetus. Science 236, 193–197.
Liu, J. P., Baker, J., Perkins, A. S., Robertson, E. J., and Efstratiadis, A. (1993) Mice carrying small mutations of the genes encoding insulin like growth factor I (IGF-I) and type 1 IGF receptor (IGF/r). Cell 75, 73–82.
Jones, J. I., Gockerman, A., Busby, W. H., Camacho-Hubner, C., and Clemmons, D. R. (1993) Extracellular matrix contains insulin-like growth factor binding protein-5, Potentiation of the effects of IGF-I. J. Cell Biol. 121, 679–687.
Mohan, S., Nakao, Y., Honda, Y., Landale, E. C., Leser, U., Dony, C., Lang, K., and Baylink, D. J. (1995) Studies on the mechanisms by which insulin-like growth factor (IGF) binding protein-4 (IGFBP-4) and IGFBP-5 modulate IGF actions in bone cells. J. Biol. Chem. 270, 20,424–20,431.
Arai, A., Busby, W. H., and Clemmons, D. R. (1996) Binding of insulin-like growth factor I or II to IGF binding protein-2 enables it to bind to heparin or extracellular matrix. Endocrinology 137, 4571–4575.
Khorsondi, M. J., Fagin, J. A., Ginnella-Neto, Forrester, J. S., and Cercek, B. (1992) Regulation of insulin-like growth factor I and its receptor in rat aorta after balloon degradation: Evidence for local bioactivity. J. Clin. Invest. 90, 1926–1931.
Knutson, B. S., Haysel, P. C., and Nachman, R. (1988) L. Plasminogen activator is associated with the extracellular matrix of cultured bovine smooth muscle cells. J. Clin. Invest. 80, 1082–1088.
Ross, R. (1971) The smooth muscle cell: Growth of smooth muscle in cultures and formation of elastic fibers. J. Cell Biol. 50, 172–186.
Hossenlopp, P., Seurin, D., Segovia-Quinson, B., Hardouin, S., and Binoux, M. (1986) Analysis of serum insulin-like growth factor binding proteins using Western blotting: Use of the method for titration of the binding proteins and competitive binding studies. Anal. Biochem. 154, 138–143.
Camacho-Hubner, C., Busby, W. H., McCusker, R. H., Wright, G., and Clemmons, D. R. (1992) Identification of the forms of insulin-like growth factor binding proteins produced by human fibroblasts and the mechanisms that regulate their secretion. J. Biol. Chem. 267, 11,949–11,956.
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© 2000 Humana Press Inc.
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Zheng, B., Clemmons, D.R. (2000). Methods for Preparing Extracellular Matrix and Quantifying Insulin-like Growth Factor-Binding Protein Binding to the ECM. In: Streuli, C.H., Grant, M.E. (eds) Extracellular Matrix Protocols. Methods in Molecular Biology™, vol 139. Humana Press. https://doi.org/10.1385/1-59259-063-2:221
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DOI: https://doi.org/10.1385/1-59259-063-2:221
Publisher Name: Humana Press
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