Migration Assays for Oligodendrocyte Precursor Cells

  • Emma E. Frost
  • Richard Milner
  • Charles ffrench-Constant
Part of the Methods in Molecular Biology™ book series (MIMB, volume 139)


In the developing central nervous system (CNS), oligodendrocyte precursor cells (OPCs) originate in discreet regions, which may be at a significant distance from their final position. These cells must, therefore, undergo extensive migration in order to reach this final destination (1). The regulation of OPC migration remains to be fully elucidated, with the role of external factors such as extracellular matrix proteins, and growth factors, as well as cell-cell interactions with other neural cells currently being investigated. In order to assess the possible regulation of OPC migration, we have refined two separate assays that present the OPCs with putative regulatory molecules in different forms.


Lower Plate Oligodendrocyte Precursor Cell Mixed Glial Culture Chemotaxis Chamber Tissue Culture Grade 
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Copyright information

© Humana Press Inc. 2000

Authors and Affiliations

  • Emma E. Frost
    • 1
  • Richard Milner
    • 1
  • Charles ffrench-Constant
    • 1
  1. 1.Wellcome Trust Cancer Research Campaign InstituteUniversity of CambridgeCambridgeUK

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